FastQCFastQC Report
Thu 26 May 2016
SRR1512198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512198_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2682817
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63710.23747426678748496No Hit
GTCCTACAGTGGACATTTCTAAATT47600.1774254449707155No Hit
CTGTAGGACGTGGAATATGGCAAGA43210.16106204784001296No Hit
TATCAACGCAGAGTACTTTTTTTTT40910.15248896961663805No Hit
GGTATCAACGCAGAGTACTTTTTTT39260.14633871784769514No Hit
CTTTAGGACGTGAAATATGGCGAGG38300.14276038954576475No Hit
GTCCTAAAGTGTGTATTTCTCATTT37710.14056120861020338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC1600.014.2455548
AATACCG759.673604E-713.9341815
AAGACCG604.0916464E-412.6674375
CAACCGT604.1045345E-412.6627176
ACTGCGT1103.8346116E-812.0871388
AACCGTG1201.0071744E-811.8712977
ATACCGT752.0809063E-411.3964436
GGCGAGG10200.011.17381819
GCACCGT1451.2259989E-911.1344576
GGTATCA17250.010.9486951
CCAACGA1658.0035534E-1110.93679619
CGACCAC700.00149590210.85375610
TCCAACG2200.010.79208818
TAGACAG4950.010.7481295
TTAGGAC14350.010.7253913
AAACCGT803.78349E-410.6841666
GGACGTG23050.010.671296
TAGGACC7100.010.5710654
AGGACGT23400.010.5561975
GACGTGA12450.010.4505557
Produced by FastQC (version 0.11.5)