FastQCFastQC Report
Thu 26 May 2016
SRR1512197_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512197_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692262
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61030.22668670434006793No Hit
GTCCTACAGTGGACATTTCTAAATT38330.14237098766761927No Hit
GGTATCAACGCAGAGTACTTTTTTT37530.13939950866594708No Hit
TATCAACGCAGAGTACTTTTTTTTT36650.13613088176410765No Hit
CTGTAGGACGTGGAATATGGCAAGA33440.12420782226989795No Hit
TTGTAGAACAGTGTATATCAATGAG29240.1086075575111189No Hit
GTCCTAAAGTGTGTATTTCTCATTT28410.105524648046884No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG602.7670454E-514.1385515
GTCTACG550.001824987612.9580361
TAGGACC6650.012.3313684
CGTTAGG751.0886682E-412.2175781
GACGTGA10600.011.3819797
CCAACGA2500.011.31126119
TCCAACG4250.011.08947118
GGCGAGG9000.010.9970619
AGGACGT20150.010.9927535
GGACGTG19550.010.9926336
CGGTTTC3700.010.95419213
CGTCCAC700.001592977710.77222910
TTAGGAC12400.010.7179353
CATTTCG804.0563813E-410.60391314
TGTAGGA20500.010.5786342
GTCCTAG4350.010.5323951
GTCCTAC20300.010.482241
GTAGGAC20400.010.4421983
TAGGACG21450.010.4144124
CTAGGAC3850.010.2825823
Produced by FastQC (version 0.11.5)