FastQCFastQC Report
Thu 26 May 2016
SRR1512197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692262
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73550.2731903507162379No Hit
TATCAACGCAGAGTACTTTTTTTTT45040.16729426779414483No Hit
GTCCTACAGTGGACATTTCTAAATT44520.16536280644305792No Hit
GGTATCAACGCAGAGTACTTTTTTT44200.16417421484238903No Hit
CTGTAGGACGTGGAATATGGCAAGA39790.14779393684567105No Hit
TTGTAGAACAGTGTATATCAATGAG34240.12717930127157015No Hit
GTCCTAAAGTGTGTATTTCTCATTT32480.12064204746789131No Hit
GATATACACTGTTCTACAAATCCCG32190.11956488632978515No Hit
CTTTAGGACGTGAAATATGGCGAGG30800.1144019415643797No Hit
GTGTATATCAATGAGTTACAATGAA27770.10314746484554624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3500.015.46681518
ATACCGC456.7799125E-414.7733736
CCAACGT2100.012.66359519
CCAACGA2300.012.38830119
TAGGACC8600.011.9304434
ACCGTCC907.4909476E-611.607658
CGAATAT752.0806442E-411.39660212
ATAGGAC3850.011.3508493
GCCGGTT3300.011.22392611
GTCCGAT600.00589061211.08002911
CGGTTTC3400.010.8938113
CCGGTTT3350.010.77290712
TGTAGGA22950.010.7627172
ACCGCTT803.7830154E-410.6843148
GTAGGAC21700.010.6822163
GGCGAGG8650.010.65059619
TGTAGAA13600.010.5479792
GGTATCA20550.010.5365791
GTCTTAG3350.010.5351931
AGGACGT19300.010.5338645
Produced by FastQC (version 0.11.5)