FastQCFastQC Report
Thu 26 May 2016
SRR1512196_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512196_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755890
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63880.23179444752874753No Hit
GTCCTACAGTGGACATTTCTAAATT57820.2098051809034468No Hit
CTGTAGGACGTGGAATATGGCAAGA52080.18897706367090122No Hit
GTCCTAAAGTGTGTATTTCTCATTT46730.169564097260776No Hit
CTTTAGGACGTGAAATATGGCGAGG44810.1625972009042451No Hit
GGTATCAACGCAGAGTACTTTTTTT38180.13853963692309926No Hit
TATCAACGCAGAGTACTTTTTTTTT38060.13810420590081607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2100.013.46015218
CGTGCGC1201.1346856E-811.77613710
GGCGAGG13950.011.6172219
CACCGTT658.6071884E-411.5953867
TAGGACC7850.011.1612814
CCAACGT1105.531092E-711.13521619
GTCAACG650.00617415511.0102052
CCCCGGT600.006248924410.99245719
GGTATCA17750.010.8285491
AAGGCGT1502.4501787E-910.6774186
GGACGTG31750.010.2669376
AGGACGT32550.010.2459675
GTCCTAT3700.010.22376251
CGTTAGG905.6794094E-410.22376251
TTAGGAC20650.010.1738543
GGACCGC750.002837957310.0493356
GTCCTAC30600.010.0232951
GACGTGA17450.09.9881467
GCGTGCG1252.8615414E-69.7977469
CTAGGAC3850.09.7881363
Produced by FastQC (version 0.11.5)