FastQCFastQC Report
Thu 26 May 2016
SRR1512196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512196_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755890
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83430.3027334182423827No Hit
GTCCTACAGTGGACATTTCTAAATT64860.23535046754406017No Hit
CTGTAGGACGTGGAATATGGCAAGA58750.21317977132614147No Hit
CTTTAGGACGTGAAATATGGCGAGG55230.20040712800583477No Hit
TATCAACGCAGAGTACTTTTTTTTT53380.19369423307896905No Hit
GTCCTAAAGTGTGTATTTCTCATTT52760.19144450613050595No Hit
GGTATCAACGCAGAGTACTTTTTTT51160.18563875916673017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3650.013.52987918
CCAACGA2400.013.05875819
TAGGACC9800.012.5062854
CGTGCAA658.0486445E-411.68853410
CAAGTCG2250.011.396326
GGCGAGG15950.010.8370219
CCCGTTT3000.010.76319112
GGTATCA19900.010.6896151
AGGACGT31650.010.6866355
AAGTCGT2400.010.684057
GGACGTG30300.010.6566256
GACGTGG14900.010.6442147
CGGTTTC1909.094947E-1210.49661213
GCCGGTT1909.094947E-1210.49661211
AGTCGTC2450.010.4660098
TGTAGGA31550.010.4193692
CTAGGAC4300.010.3847033
TTAGGAC19400.010.3824253
TCCCGTT3150.010.25065911
AGGACCT20300.010.1561335
Produced by FastQC (version 0.11.5)