FastQCFastQC Report
Thu 26 May 2016
SRR1512195_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512195_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771046
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43580.246069272057304No Hit
TATCAACGCAGAGTACTTTTTTTTT26680.15064543778083686No Hit
GGTATCAACGCAGAGTACTTTTTTT25390.1433616066437574No Hit
GTCCTACAGTGGACATTTCTAAATT19640.11089491746685291No Hit
CTGTAGGACGTGGAATATGGCAAGA18620.10513560912590639No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG500.001594635513.1920634
AAGACCG701.1772667E-412.115165
TGTGCGA701.177887E-412.11447510
GGTATCA8350.012.1025851
GTATCAA18600.011.085821
GTCCTAA5100.011.0083421
GGCGAGG5300.010.84614619
TTAGGAC7400.010.8236053
TAGGACC4200.010.7690314
ACTAGAC804.0660397E-410.6007653
TACACCG804.0660397E-410.6007655
GTCCTAC10950.010.5340111
GACGTGA6650.010.4856367
TTAGACT1451.6383638E-810.3976854
TCCAACG1002.6220541E-510.36607118
TACCCTA1002.6245049E-510.3651925
CTAGGAC2100.010.3203213
TAGGAGT1651.0695658E-910.279534
TGAAATA8750.010.22971311
AGGACGT11900.010.2147435
Produced by FastQC (version 0.11.5)