##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512195_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1771046 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.51409788339772 31.0 28.0 33.0 16.0 34.0 2 25.63960281099418 31.0 25.0 33.0 2.0 34.0 3 27.29858004817492 31.0 26.0 34.0 16.0 34.0 4 31.763225235256453 35.0 32.0 37.0 19.0 37.0 5 32.14617915062624 35.0 33.0 37.0 22.0 37.0 6 32.346246794267344 35.0 33.0 37.0 23.0 37.0 7 32.312161287736174 35.0 33.0 37.0 23.0 37.0 8 32.31342551238082 35.0 33.0 37.0 23.0 37.0 9 33.72851919148345 38.0 34.0 39.0 23.0 39.0 10 33.574529967036426 38.0 33.0 39.0 22.0 39.0 11 33.59004001025383 38.0 33.0 39.0 22.0 39.0 12 33.43113786993675 38.0 33.0 39.0 20.0 39.0 13 33.41969943186117 38.0 33.0 39.0 19.0 39.0 14 34.37049348238273 39.0 33.0 41.0 19.0 41.0 15 34.271452011974844 39.0 33.0 41.0 18.0 41.0 16 34.20699462351627 39.0 33.0 41.0 18.0 41.0 17 34.16154521113511 39.0 33.0 41.0 17.0 41.0 18 34.09270058485212 38.0 33.0 41.0 17.0 41.0 19 34.09979808542522 39.0 33.0 41.0 18.0 41.0 20 33.99061289204233 39.0 33.0 41.0 17.0 41.0 21 33.893361888962794 39.0 33.0 41.0 16.0 41.0 22 33.76597558730829 39.0 33.0 40.0 10.0 41.0 23 33.620041489605576 39.0 33.0 40.0 8.0 41.0 24 33.46216473202842 38.0 33.0 40.0 2.0 41.0 25 33.299814911639785 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9972812819741677 1101 2 3.793455990416536 1101 3 2.450520103424651 1101 4 1.356736486456672 1101 5 1.186415966241654 1101 6 1.3094840092980817 1101 7 1.0324008604102417 1101 8 1.0720993140242498 1101 9 0.8771792722739775 1101 10 1.0969273195401357 1101 11 1.171613432810318 1101 12 1.1048171971275451 1101 13 1.0977072973976192 1101 14 1.0103795226768995 1101 15 1.1200905904516674 1101 16 1.5445976988094898 1101 17 1.2168859337894702 1101 18 1.0181481221022395 1101 19 0.8759280185873095 1101 20 1.1455954988357249 1101 21 1.2926532425269386 1101 22 1.190906567696004 1101 23 1.2689583975776273 1101 24 1.0649070647432808 1101 25 1.2867033067424813 1102 1 1.7546576744367641 1102 2 4.5109777856058315 1102 3 2.788935817982548 1102 4 1.9955223969880755 1102 5 1.373491763782674 1102 6 1.4942501687788479 1102 7 1.3409434772910416 1102 8 1.3539315266732999 1102 9 1.3701720047682073 1102 10 1.2119928413338243 1102 11 1.3836845047251387 1102 12 1.2816661007259 1102 13 1.225806904202976 1102 14 1.4606743904777417 1102 15 1.6015941325104919 1102 16 1.4085455113612824 1102 17 1.4752542380093132 1102 18 1.6955421168178049 1102 19 1.4287282010735183 1102 20 1.4826902928303625 1102 21 1.5687666019379591 1102 22 1.3983313062270142 1102 23 1.5215236704069142 1102 24 1.613348773063727 1102 25 1.44724645123992 1103 1 1.2025009714854882 1103 2 4.09197003507661 1103 3 2.6302455424192175 1103 4 1.720535849317141 1103 5 1.3739684518547506 1103 6 1.1573850370738086 1103 7 1.0792470498604985 1103 8 1.2336822575003552 1103 9 1.2046842211867244 1103 10 1.2598306012761213 1103 11 1.2082757171952707 1103 12 1.2606201271251223 1103 13 1.368664604827245 1103 14 1.304525859727633 1103 15 1.6186470005186493 1103 16 1.4200211644203904 1103 17 1.5398822083474641 1103 18 1.5780699194810168 1103 19 1.732905947868737 1103 20 1.789086076678764 1103 21 1.7647663265024107 1103 22 1.7921274754012302 1103 23 1.6151561399989731 1103 24 1.694914964911682 1103 25 1.6322855446310314 1104 1 1.411436915093784 1104 2 4.225711033191988 1104 3 2.7480986388733797 1104 4 1.798555302956654 1104 5 1.542933115513975 1104 6 1.2418897306041004 1104 7 1.3844215127289843 1104 8 1.2320850426935124 1104 9 1.3997247079992121 1104 10 1.501122018959414 1104 11 1.4219651689903827 1104 12 1.4473746504291327 1104 13 1.4597197165386788 1104 14 1.6638350530561539 1104 15 1.3054611550244815 1104 16 1.611993764576539 1104 17 1.570238650389399 1104 18 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-1.4274823962057752 2316 6 -1.4348819535791435 2316 7 -1.7241139472865932 2316 8 -1.6214943126193617 2316 9 -1.8016260605707828 2316 10 -1.8221165370453463 2316 11 -2.5080109625942804 2316 12 -1.8695644344959668 2316 13 -2.014978534378699 2316 14 -2.203977578420549 2316 15 -2.4348696206024663 2316 16 -2.1026452570934566 2316 17 -2.3109014394784886 2316 18 -2.817606137500192 2316 19 -2.6266172779578696 2316 20 -2.5362821933354383 2316 21 -2.4600490277554385 2316 22 -2.319767964515897 2316 23 -2.2667991700832992 2316 24 -2.079501775661349 2316 25 -1.9337488921303603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 90631.0 3 1.0 4 845.0 5 3962.0 6 4634.0 7 4161.0 8 3970.0 9 3911.0 10 3883.0 11 3685.0 12 3673.0 13 3744.0 14 3728.0 15 3799.0 16 4089.0 17 4322.0 18 4849.0 19 6022.0 20 7722.0 21 9963.0 22 12549.0 23 15074.0 24 17406.0 25 19611.0 26 22101.0 27 24636.0 28 28480.0 29 33824.0 30 41534.0 31 51766.0 32 64632.0 33 82546.0 34 107713.0 35 144843.0 36 194923.0 37 245503.0 38 492311.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.5407098403994 18.47371553308045 12.358459351140512 25.627115275379637 2 16.933346463941497 20.381170952566492 38.51431654267009 24.17116604082192 3 19.235017046423415 23.364384663074816 29.270894149559073 28.129704140942696 4 13.047148408341739 15.651936765052968 36.60751894643053 34.69339588017476 5 13.922168029514761 37.00835551419895 35.01868387382372 14.050792582462568 6 33.61109762253493 35.742380491528735 17.069630037842042 13.576891848094292 7 29.234814073620658 31.145858979194944 21.275938553833157 18.343388393351237 8 26.239295873737895 36.148242338143675 19.698189657411497 17.914272130706937 9 26.61342506067036 15.351662238022051 19.514569356188378 38.520343345119215 10 15.693157807363372 28.361157820915178 33.326595075500485 22.619089296220967 11 35.52697553715051 22.006457324964483 23.440722027857518 19.025845110027486 12 23.653223748252444 23.977100512466095 30.783650773216248 21.586024966065214 13 29.43435687158888 21.158456640877763 25.384885542216296 24.02230094531706 14 21.655915010629318 21.05079204650362 26.70462918785237 30.588663755014693 15 23.714347340498215 29.79228094583653 22.8413604163867 23.65201129727856 16 23.26653288508599 27.423341912067784 26.26075212049828 23.049373082347945 17 22.502125862343497 27.77923328925392 27.005679129734634 22.71296171866795 18 22.564436460846835 26.786864861171424 28.461481557193057 22.18721712078868 19 24.032406696360006 26.397804168511207 26.96850126934509 22.601287865783696 20 24.064648800765198 26.417834432307234 27.39923186636598 22.118284900561587 21 23.554064813403127 26.120908223362864 26.77367259010802 23.551354373125985 22 23.944617101144203 26.717206394521565 26.77220307439346 22.56597342994078 23 23.221382149169116 26.611425118999893 27.24631707330845 22.920875658522537 24 23.700674417698508 25.980017629842205 27.55614359342715 22.76316435903213 25 23.41663970347834 26.385696127408547 27.339825956148538 22.85783821296458 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 533.0 1 533.0 2 552.0 3 571.0 4 571.0 5 571.0 6 1402.0 7 2233.0 8 2233.0 9 2233.0 10 2942.5 11 3652.0 12 3652.0 13 3652.0 14 5407.5 15 7163.0 16 7163.0 17 7163.0 18 12380.0 19 17597.0 20 17597.0 21 17597.0 22 27165.0 23 36733.0 24 36733.0 25 36733.0 26 52980.5 27 69228.0 28 69228.0 29 69228.0 30 89983.5 31 110739.0 32 110739.0 33 110739.0 34 127325.5 35 143912.0 36 143912.0 37 143912.0 38 160355.5 39 176799.0 40 176799.0 41 176799.0 42 192266.0 43 207733.0 44 207733.0 45 207733.0 46 218049.0 47 228365.0 48 228365.0 49 228365.0 50 227749.5 51 227134.0 52 227134.0 53 227134.0 54 209909.5 55 192685.0 56 192685.0 57 192685.0 58 169779.0 59 146873.0 60 146873.0 61 146873.0 62 122670.5 63 98468.0 64 98468.0 65 98468.0 66 77267.5 67 56067.0 68 56067.0 69 56067.0 70 41680.0 71 27293.0 72 27293.0 73 27293.0 74 19287.5 75 11282.0 76 11282.0 77 11282.0 78 7713.5 79 4145.0 80 4145.0 81 4145.0 82 2748.5 83 1352.0 84 1352.0 85 1352.0 86 860.5 87 369.0 88 369.0 89 369.0 90 230.0 91 91.0 92 91.0 93 91.0 94 56.0 95 21.0 96 21.0 97 21.0 98 14.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.6897494475016455 3 0.0 4 0.0 5 0.0 6 0.0 7 0.007340294944343625 8 0.0 9 0.0 10 0.0036136836649076306 11 0.0019197694469821787 12 1.1292761452836346E-4 13 0.0 14 5.646380726418173E-5 15 0.0 16 0.0 17 0.0 18 0.010897514801987074 19 0.0030490455922658134 20 0.0 21 0.0065498016426450804 22 0.001693914217925452 23 0.0020326970615105423 24 0.01044580434387362 25 0.006380410220852536 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1771046.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.89199007671284 #Duplication Level Percentage of deduplicated Percentage of total 1 84.26658833116775 62.26625908765093 2 10.486276793542542 15.497037215402193 3 2.6646651627607176 5.906922352934321 4 1.0286508054246004 3.0403622042734897 5 0.49452935176425494 1.8270878976603782 6 0.2862385795301445 1.2690442966928288 7 0.18774128272873336 0.9710803900266635 8 0.12500625756463418 0.7389568914794367 9 0.08840023710036915 0.5878862498339585 >10 0.34779403316898205 4.243994843049074 >50 0.013284685845214143 0.6892137411160997 >100 0.009278473740444545 1.3921326419330327 >500 7.730028307752594E-4 0.40382933273147875 >1k 7.730028307752579E-4 1.166192855216208 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4358 0.246069272057304 No Hit TATCAACGCAGAGTACTTTTTTTTT 2668 0.15064543778083686 No Hit GGTATCAACGCAGAGTACTTTTTTT 2539 0.1433616066437574 No Hit GTCCTACAGTGGACATTTCTAAATT 1964 0.11089491746685291 No Hit CTGTAGGACGTGGAATATGGCAAGA 1862 0.10513560912590639 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 5.6463807264181735E-5 0.0 13 0.0 0.0 0.0 5.6463807264181735E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGACG 50 0.0015946355 13.192063 4 AAGACCG 70 1.1772667E-4 12.11516 5 TGTGCGA 70 1.177887E-4 12.114475 10 GGTATCA 835 0.0 12.102585 1 GTATCAA 1860 0.0 11.08582 1 GTCCTAA 510 0.0 11.008342 1 GGCGAGG 530 0.0 10.846146 19 TTAGGAC 740 0.0 10.823605 3 TAGGACC 420 0.0 10.769031 4 ACTAGAC 80 4.0660397E-4 10.600765 3 TACACCG 80 4.0660397E-4 10.600765 5 GTCCTAC 1095 0.0 10.534011 1 GACGTGA 665 0.0 10.485636 7 TTAGACT 145 1.6383638E-8 10.397685 4 TCCAACG 100 2.6220541E-5 10.366071 18 TACCCTA 100 2.6245049E-5 10.365192 5 CTAGGAC 210 0.0 10.320321 3 TAGGAGT 165 1.0695658E-9 10.27953 4 TGAAATA 875 0.0 10.229713 11 AGGACGT 1190 0.0 10.214743 5 >>END_MODULE