FastQCFastQC Report
Thu 26 May 2016
SRR1512195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512195_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771046
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51930.29321655112289574No Hit
TATCAACGCAGAGTACTTTTTTTTT33330.1881938696115177No Hit
GGTATCAACGCAGAGTACTTTTTTT32570.1839026202594399No Hit
GTCCTACAGTGGACATTTCTAAATT24470.13816693637545271No Hit
CTGTAGGACGTGGAATATGGCAAGA21930.12382512933035053No Hit
GTCCTAAAGTGTGTATTTCTCATTT19650.11095138127411712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4500.012.666634
GGTATCA10750.012.3373411
ACTATCG701.09426495E-412.21045611
CTTATAC1900.010.9999683
TCCAACG1053.4795466E-610.85373918
TCCGCGC700.001495487510.85373816
CTACGGA803.7713358E-410.6874694
GTCCTAC12600.010.6016331
TTAGGAC6850.010.5401143
GTATTAA2450.010.5150891
CTATACT1553.6616257E-910.4193254
CTACACT3100.010.4193254
GACGTGG6500.010.2275627
ACCGTGC1303.8577673E-710.2275628
GTCTAGG1502.2844688E-810.1775671
AGGACGT12000.010.1333035
TACACCG750.00264794910.1333035
TCTATAC1502.4445399E-810.1333033
GGATCGT750.002654780910.1301566
GGACGTA750.002654780910.1301566
Produced by FastQC (version 0.11.5)