FastQCFastQC Report
Thu 26 May 2016
SRR1512194_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512194_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2368962
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT52800.2228824269870095No Hit
GTATCAACGCAGAGTACTTTTTTTT52240.2205185224583594No Hit
CTGTAGGACGTGGAATATGGCAAGA47690.20131179816307732No Hit
GTCCTAAAGTGTGTATTTCTCATTT43880.1852288048520829No Hit
CTTTAGGACGTGAAATATGGCGAGG40350.1703277638054135No Hit
TATCAACGCAGAGTACTTTTTTTTT33540.14158099623379353No Hit
GGTATCAACGCAGAGTACTTTTTTT32200.1359245103973808No Hit
GTCCTACAGTGTGCATTTCTCATTT24250.10236550860672311No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC350.002286684516.1592147
CGGTAGG754.4577428E-714.9228341
ACTATCG653.6589936E-614.50155211
TGATCGG400.00556123714.13901310
TAGGACC8250.013.8251054
CCAACGA1800.012.56933819
TCCAACG2800.012.12043418
TGTAGGA28100.011.5868022
TCCCGTT1751.8189894E-1211.3112111
ACCGTCC752.230902E-411.311218
CTGTAGG27950.011.139341
CTATCGG855.7629404E-511.09012412
CCCGTTT2050.011.03602712
GGCGAGG11850.010.97828219
ATCCCGT2000.010.8399110
GGTATCA13800.010.7644961
GTAGGAC28650.010.6929053
GTCCTAC27300.010.6591671
CTAGGAC3400.010.5351723
AGGACGT27500.010.4887975
Produced by FastQC (version 0.11.5)