FastQCFastQC Report
Thu 26 May 2016
SRR1512194_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512194_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2368962
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62980.26585483431139884No Hit
GTCCTACAGTGGACATTTCTAAATT59020.2491386522873731No Hit
CTGTAGGACGTGGAATATGGCAAGA52990.22368446602351577No Hit
CTTTAGGACGTGAAATATGGCGAGG48700.20557526883082125No Hit
GTCCTAAAGTGTGTATTTCTCATTT48240.20363349011085868No Hit
TATCAACGCAGAGTACTTTTTTTTT40140.16944129960716972No Hit
GGTATCAACGCAGAGTACTTTTTTT39650.1673728831446009No Hit
GTCCTACAGTGTGCATTTCTCATTT27390.11562025899951119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9750.013.6416464
TCCAACG2850.012.66269818
GGCGAGG13200.011.08056319
TATATCG700.001491335510.8576375
GGACGTA700.00149580710.8537426
GTCTAGG2050.010.7028621
GTCCTAG3400.010.6618031
AGGACCT19750.010.53465755
AATGTCC8000.010.3280148
GGTATCA15600.010.2121661
GGACCTG19350.010.2086886
TGGCGAG29050.010.19990218
TGTAGGA33650.010.19214252
CTGTAGG32450.010.17155551
TGTCCAC9450.010.15025910
GTAGGAC31850.010.1417493
CCAACGA2250.010.13080119
ATACGGC750.002655340810.13015815
AAATGTC7600.010.1218287
GTCCTAC28050.09.9306111
Produced by FastQC (version 0.11.5)