FastQCFastQC Report
Thu 26 May 2016
SRR1512193_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512193_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990947
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46410.23310515046357336No Hit
GTCCTACAGTGGACATTTCTAAATT36080.18122029365924858No Hit
CTGTAGGACGTGGAATATGGCAAGA30370.15254047445763247No Hit
GGTATCAACGCAGAGTACTTTTTTT29200.14666387402577769No Hit
TATCAACGCAGAGTACTTTTTTTTT27680.1390293161997783No Hit
GTCCTAAAGTGTGTATTTCTCATTT22590.11346359295350404No Hit
CTTTAGGACGTGAAATATGGCGAGG20650.10371948625453115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAA457.211474E-414.65326911
TGCACCG958.238567E-812.8924135
TAGGACC7250.012.8650544
TCCAACG2400.012.17080918
ACCGATC550.00326843411.9896418
CCAACGA1900.011.40625919
GTAGGAC18950.011.1863343
GTAGAAC7450.011.1286083
GGTCTAC2300.011.1129491
GTCCTAC18850.011.0646071
GGTATCA11950.011.0366941
CAGCGAC600.00625148510.9916097
TTACGCT600.00625148510.9916094
GGCGAGG7550.010.98258919
TGTAGGA19100.010.8662532
CTGTAGG18850.010.7934161
AGGACGT16700.010.7753495
CGTGTTT700.001597089610.76864219
TAGAACA8400.010.767294
TCCTACA19700.010.7428942
Produced by FastQC (version 0.11.5)