FastQCFastQC Report
Thu 26 May 2016
SRR1512193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512193_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990947
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61590.3093502740153304No Hit
GTCCTACAGTGGACATTTCTAAATT41480.20834306488319376No Hit
GGTATCAACGCAGAGTACTTTTTTT39970.2007587344113128No Hit
TATCAACGCAGAGTACTTTTTTTTT36440.1830284784075116No Hit
CTGTAGGACGTGGAATATGGCAAGA35300.17730256003801206No Hit
GTCCTAAAGTGTGTATTTCTCATTT24890.12501588440073994No Hit
CTTTAGGACGTGAAATATGGCGAGG23780.11944064809359567No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20340.1021624382768602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7050.014.2841984
TCCAACG2500.013.29613218
GGATCGT550.003075658612.0873946
GTAGGAC19200.011.9249043
TGTAGGA20800.011.7840752
GTATTAG1604.1836756E-1111.3251841
CTGTAGG21200.011.2014731
CCGTAGG600.005889378511.080119
TAGGACT3050.010.9020284
CCGGACT700.00149127710.8575314
CCAACGT1053.4772129E-610.85453119
CCAACGA2200.010.79285819
AGGACCG1158.8152956E-710.7395145
AGGACGT16600.010.7021895
AGGACCT13450.010.6658085
GTGTAGG3500.010.626941
GGACCTG13550.010.5836376
ACTGTTC7900.010.4589838
TAGGACG17900.010.4556814
GGCGAGA11200.010.43052619
Produced by FastQC (version 0.11.5)