FastQCFastQC Report
Thu 26 May 2016
SRR1512192_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512192_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217435
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40941.8828615448294894No Hit
GGTATCAACGCAGAGTACTTTTTTT27701.273943937268609No Hit
TATCAACGCAGAGTACTTTTTTTTT27031.2431301308437004No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT19190.8825626049164118No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA18610.8558879665187298No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13880.6383516913100467No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13300.6116770529123646No Hit
GTACATAAGCAGTGGTATCAACGCA10730.49348081035711827No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9000.4139168027226528No Hit
GTGGTATCAACGCAGAGTACATGGG5890.2708855520040472No Hit
GCAGAGTACTTTTTTTTTTTTTTTT5240.24099156069630007No Hit
GTACTGGTTCACTATCGGTCAGTCA5140.23639248511049277No Hit
GCTTATGTACTCTGCGTTGATACCA5100.2345528548761699No Hit
GTGGTATCAACGCAGAGTACTTTTT5100.2345528548761699No Hit
GGTATCAACGCAGAGTACATGGGGG4510.20741830891990712No Hit
ATCAACGCAGAGTACTTTTTTTTTT4260.19592061995538898No Hit
GNATCAACGCAGAGTACTTTTTTTT3930.18074367052222504No Hit
ATACAGGGTGACAGCCCCGTACACA3800.1747648722606756No Hit
CTTATGTACTCTGCGTTGATACCAC3590.16510681353048037No Hit
GGTATCAACGCAGAGAAAAAAAAAA3470.15958792282751166No Hit
GAGTACATAAGCAGTGGTATCAACG3330.15314921700738152No Hit
GGATACCACGTGTCCCGCCCTACTC3270.15038977165589715No Hit
GTATCAACGCAGAGTACATGGGGGT2880.13245337687124886No Hit
ATCATTAACTGAATCCATAGGTTAA2860.13153356175408742No Hit
GGTATCAACGCAGAGTACATGGGGA2860.13153356175408742No Hit
TNTCAACGCAGAGTACTTTTTTTTT2790.12831420884402236No Hit
TATGTACTCTGCGTTGATACCACTG2780.12785430128544165No Hit
GTACATGGGGGTTAAGCGACTAAGC2720.12509485593395728No Hit
GTATCAACGCAGAGAAAAAAAAAAA2700.12417504081679583No Hit
GGTATCAACGCAGAAAAAAAAAAAA2620.12049578034815003No Hit
ACTTAGATGTTTCAGTTCCCCCGGT2580.11865615011382712No Hit
GNTATCAACGCAGAGTACTTTTTTT2570.11819624255524638No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA2350.10807827626647043No Hit
GGTTAATGAGGCGAACCGGGGGAAC2230.10255938556350172No Hit
ACCCTGTATCGCGCGCCTTTCCAGA2210.10163957044634028No Hit
TNTTTTTTTTTTTTTTTTTTTTTTT2200.10117966288775955No Hit
AGCGTACACGGTGGATGCCCTGGCA2190.10071975532917883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTC250.006322339618.8371665
ACAGAGG400.005568817314.1278743
TACAGAG600.003306864511.9622762
GACTAAG803.1013085E-511.77322918
GTACTGG1205.6093086E-711.1078281
TTAACCT600.00623497410.9883474
CGCCCTA951.4798565E-510.90572816
TGTCCCG951.4798565E-510.90572811
AAGCGAC804.0523772E-410.59590614
GCGACTA901.028752E-410.46509316
GTACATA2550.010.4544261
TCCCGCC1002.6087746E-510.36044113
CCGCCCT1002.6087746E-510.36044115
ACATAAG2600.010.1430893
GCAGTCA750.002826677910.04648913
TTAAGCG750.002826677910.04648912
TACATAA2450.010.0441282
ACTAAGC857.083218E-49.97261719
CGCAGAA857.083218E-49.9726179
TAAGCGA857.083218E-49.97261713
Produced by FastQC (version 0.11.5)