FastQCFastQC Report
Thu 26 May 2016
SRR1512192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512192_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217435
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51072.348747901671764No Hit
TATCAACGCAGAGTACTTTTTTTTT38231.7582265964541128No Hit
GGTATCAACGCAGAGTACTTTTTTT37451.7223538068848159No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT22781.0476694184468922No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA22461.032952376572309No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19660.9041782601697058No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15190.6985995814841217No Hit
GTACATAAGCAGTGGTATCAACGCA13200.6070779773265573No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10570.4861222894198266No Hit
GTGGTATCAACGCAGAGTACATGGG7720.35504863522431995No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6710.3085979718076667No Hit
GTGGTATCAACGCAGAGTACTTTTT6620.30445880378044016No Hit
GTACTGGTTCACTATCGGTCAGTCA6290.2892818543472762No Hit
GGTATCAACGCAGAGTACATGGGGG6100.2805436107342424No Hit
ATCAACGCAGAGTACTTTTTTTTTT5810.2672062915354014No Hit
GCTTATGTACTCTGCGTTGATACCA5310.24421091360636513No Hit
ATACAGGGTGACAGCCCCGTACACA4770.21937590544300595No Hit
CTTATGTACTCTGCGTTGATACCAC4110.1890220065766781No Hit
GGATACCACGTGTCCCGCCCTACTC3820.17568468737783705No Hit
GGTATCAACGCAGAGAAAAAAAAAA3680.1692459815577069No Hit
GAGTACATAAGCAGTGGTATCAACG3590.16510681353048037No Hit
GGTATCAACGCAGAAAAAAAAAAAA3560.1637270908547382No Hit
GTATCAACGCAGAGTACATGGGGGT3470.15958792282751166No Hit
ATCATTAACTGAATCCATAGGTTAA3400.1563685699174466No Hit
TATGTACTCTGCGTTGATACCACTG3120.14349115827718628No Hit
GTACATGGGGGTTAAGCGACTAAGC3020.13889208269137904No Hit
ACTTAGATGTTTCAGTTCCCCCGGT2990.13751236001563685No Hit
AGCGTACACGGTGGATGCCCTGGCA2920.13429300710557177No Hit
GTATCAACGCAGAGAAAAAAAAAAA2800.12877411640260308No Hit
CATAAGCAGTGGTATCAACGCAGAG2740.12601467105111872No Hit
GGTATCAACGCAGAGTACATGGGGA2710.12463494837537654No Hit
GTTAATGATAGTGTGTCGAAACACA2670.12279531814105366No Hit
GGTTAATGAGGCGAACCGGGGGAAC2520.11589670476234276No Hit
CAACGCAGAGTACTTTTTTTTTTTT2430.11175753673511625No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2420.11129762917653553No Hit
GTTCACTATCGGTCAGTCAGGAGTA2380.10945799894221261No Hit
TATTCAGACAGGATACCACGTGTCC2370.10899809138363188No Hit
GTGGATGCCCTGGCAGTCAGAGGCG2330.10715846114930899No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT2300.10577873847356682No Hit
GTATTTAGCCTTGGAGGATGGTCCC2260.10393910823924392No Hit
TCTAAGTACCCCGAGGAAAAGAAAT2200.10117966288775955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCCA250.00602587318.994489
GCCCCGT1102.7030183E-912.95078214
CCCGTAC1102.7030183E-912.95078216
CCGTACA1102.7030183E-912.95078217
AGGGTGA1155.2023097E-912.3934045
CAGGGTG1155.2023097E-912.3934044
CCCCGTA1155.2386895E-912.38770415
TACTGGT1800.012.1409462
GTGCAGA550.003060528812.08739611
TGGTTCA1750.011.9448815
ACCGGGG657.9942885E-411.68891115
GTACTGG1800.011.6452331
ACAGCCC1156.9772796E-811.56185711
GTCAGTC1657.2759576E-1211.51180618
AGCCCCG1251.8026185E-811.396687513
TCGGTCA1751.8189894E-1211.396687515
ACAGGGT1201.2499913E-711.0852123
ACTGGTT1900.011.0018653
ACTATCG1900.010.99680411
GGTTCAC1900.010.9968046
Produced by FastQC (version 0.11.5)