##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512192_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 217435 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.883096097684366 31.0 31.0 34.0 28.0 34.0 2 31.002009796030997 31.0 31.0 34.0 27.0 34.0 3 31.025497275047716 31.0 31.0 34.0 27.0 34.0 4 34.72182031411686 37.0 35.0 37.0 32.0 37.0 5 34.337631935980866 37.0 35.0 37.0 30.0 37.0 6 34.31569434543657 37.0 35.0 37.0 30.0 37.0 7 34.13707544783499 37.0 35.0 37.0 30.0 37.0 8 34.06489295651574 37.0 35.0 37.0 30.0 37.0 9 35.49002230551659 39.0 35.0 39.0 30.0 39.0 10 35.334840297100286 39.0 35.0 39.0 30.0 39.0 11 35.31308207050383 39.0 35.0 39.0 30.0 39.0 12 35.13326741324994 39.0 35.0 39.0 29.0 39.0 13 35.06671419044772 38.0 35.0 39.0 28.0 39.0 14 35.959417757030835 39.0 36.0 41.0 27.0 41.0 15 35.81394899625175 39.0 36.0 41.0 27.0 41.0 16 35.69169176995424 39.0 35.0 41.0 27.0 41.0 17 35.79682663784579 39.0 36.0 41.0 27.0 41.0 18 35.732053257295284 39.0 35.0 41.0 27.0 41.0 19 35.80783222572263 39.0 35.0 41.0 27.0 41.0 20 35.75428518867707 39.0 35.0 41.0 27.0 41.0 21 35.644321291420425 39.0 35.0 41.0 27.0 41.0 22 35.654756593924624 39.0 35.0 41.0 27.0 41.0 23 35.48624646446064 39.0 35.0 41.0 27.0 41.0 24 35.33881849748201 39.0 35.0 41.0 27.0 41.0 25 34.9536275208683 39.0 35.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.22709401254599726 1101 2 -0.06656489187989578 1101 3 0.24637219966860968 1101 4 -0.11802302081749616 1101 5 -0.22077268337416456 1101 6 -0.1720382776684346 1101 7 -0.18660568276785483 1101 8 -0.5463282154158193 1101 9 -0.6546228360501232 1101 10 -0.804022557729148 1101 11 -0.6176743265743028 1101 12 -0.5079056887560398 1101 13 -0.4401384826516974 1101 14 -0.45325289140141223 1101 15 -0.38434971901715187 1101 16 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1103 3 0.4102738014992724 1103 4 0.351370571859853 1103 5 0.2908977971750346 1103 6 0.5540486788533059 1103 7 0.2923416856531986 1103 8 0.4568754459114075 1103 9 0.6316471868331774 1103 10 0.3083344216758874 1103 11 0.5574400899016752 1103 12 0.8606297803515162 1103 13 1.0699873754718752 1103 14 0.8288981383468652 1103 15 0.48561595604005703 1103 16 1.400429579470483 1103 17 1.397970175517493 1103 18 0.4012838779069625 1103 19 1.135085733200036 1103 20 0.9716946928867216 1103 21 1.3246064081634188 1103 22 1.192992886358951 1103 23 0.9799307673865982 1103 24 0.770444588585903 1103 25 1.0252271748081583 1104 1 0.4513842483235706 1104 2 0.34017423855488715 1104 3 0.10658959097295906 1104 4 0.5461074139651103 1104 5 0.753792534017137 1104 6 0.7590486788533042 1104 7 0.8283943172321457 1104 8 0.913454393279828 1104 9 0.7955945552542261 1104 10 0.6472817900969403 1104 11 0.9142821951648301 1104 12 0.9690508329830934 1104 13 1.0705136912613469 1104 14 1.32152971729424 1104 15 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15 -0.9719828602778477 2313 16 -0.9329248910431502 2313 17 -0.6992819910434847 2313 18 -0.7283715860541449 2313 19 -0.6879326561468204 2313 20 -0.4149254719832811 2313 21 -0.4607455258885764 2313 22 -0.4125873292402886 2313 23 -0.5948525765153292 2313 24 -0.39146621251322955 2313 25 -0.18555278714491408 2314 1 -0.3042721223328009 2314 2 -0.34212305874241267 2314 3 -0.7583911040077389 2314 4 -0.5067883389306402 2314 5 -0.8261109408863376 2314 6 -0.9328239080192802 2314 7 -0.7117022078643771 2314 8 -0.5555610507356121 2314 9 -0.7477066030469288 2314 10 -0.37024716743201935 2314 11 -0.540852939970307 2314 12 -0.6149646110323488 2314 13 -0.42836661761896266 2314 14 -0.8717521359876201 2314 15 -0.48408532418753936 2314 16 -0.7444799917548792 2314 17 -0.8946735960736447 2314 18 -0.6439101883397385 2314 19 -0.8073446671251006 2314 20 -0.9592339801472107 2314 21 -0.5047981843990712 2314 22 -0.5627478167501678 2314 23 -0.6811746989820264 2314 24 -1.0940138548199059 2314 25 -1.0825151539867974 2315 1 -0.3116645321642366 2315 2 -0.2887282004695031 2315 3 -0.23414211634411686 2315 4 -0.4083641307503356 2315 5 -0.3552725066332698 2315 6 -0.5492033536670178 2315 7 -0.8654674713857418 2315 8 -0.5058545498095981 2315 9 -0.6016005666969946 2315 10 -0.5549946326672881 2315 11 -0.763441382070944 2315 12 -0.5083475410006457 2315 13 -0.3999741136167003 2315 14 -0.7826979249821875 2315 15 -0.3801521226933673 2315 16 0.03235084605156402 2315 17 -0.27049366701567124 2315 18 -0.34488642590134333 2315 19 -0.31972813072807327 2315 20 -0.2759304675754066 2315 21 -0.2667500317167679 2315 22 -0.2423943622503728 2315 23 -0.006205304500440434 2315 24 -0.5378566523212385 2315 25 -0.41118703143916235 2316 1 -1.2785769919865082 2316 2 -0.8781590947784466 2316 3 -0.761084827631695 2316 4 -0.3895515007635666 2316 5 -0.9907423497037868 2316 6 -0.9157187630071562 2316 7 -0.95017157424072 2316 8 -0.6890649865651284 2316 9 -0.6475449796294939 2316 10 -1.071632938585232 2316 11 -0.5491286575483514 2316 12 -0.5895538181796951 2316 13 -1.1001064637774078 2316 14 -1.611493538519717 2316 15 -1.6300103189497435 2316 16 -1.426620604128054 2316 17 -1.076978824890503 2316 18 -1.511364020909845 2316 19 -1.3923316474609138 2316 20 -1.3357716473825576 2316 21 -0.6280659704575555 2316 22 -0.7172478317152979 2316 23 -0.8484250057672185 2316 24 -1.5618687529073654 2316 25 -1.098273437186947 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3223.0 3 0.0 4 59.0 5 559.0 6 659.0 7 494.0 8 420.0 9 406.0 10 385.0 11 360.0 12 338.0 13 308.0 14 346.0 15 314.0 16 307.0 17 349.0 18 377.0 19 453.0 20 547.0 21 693.0 22 942.0 23 1176.0 24 1339.0 25 1604.0 26 1948.0 27 2239.0 28 2635.0 29 3345.0 30 4213.0 31 5306.0 32 6842.0 33 9195.0 34 11754.0 35 15855.0 36 24269.0 37 39076.0 38 75100.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.34841451303725 18.943624564077695 14.97447978013349 19.733481142751565 2 16.60358267988134 21.873203486099293 38.482764964242186 23.040448869777176 3 19.957228597051994 27.18467588014809 30.49693011704647 22.361165405753443 4 13.025041966564721 18.362729091452618 34.78096902522593 33.831259916756736 5 12.70431282346176 38.79010925819436 32.76733755031627 15.738240368027604 6 29.85535907282636 35.81990020005979 18.233954975050015 16.090785752063834 7 25.781497918918294 35.32182031411686 20.396440315496587 18.500241451468256 8 27.06372019224136 32.40232713224642 20.325154643916573 20.20879803159565 9 27.80831052958355 15.361832271713386 20.113597166969438 36.71626003173362 10 18.75181088601191 28.550601329132846 28.86057902361625 23.83700876123899 11 32.51132522363005 24.80005518890703 21.383861843769402 21.304757743693518 12 24.968381355347574 27.43118633154736 27.88695472210086 19.713477591004207 13 30.23938188424127 22.049807988594292 26.93126681537011 20.77954331179433 14 23.638328695932117 24.322211235541655 27.90811046979557 24.131349598730655 15 26.13102766343965 27.700232253317083 26.30211327523168 19.86662680801159 16 21.162186400533493 29.113988088394233 27.568238784004418 22.15558672706786 17 20.46496654172511 28.87805551084232 27.705751144020052 22.951226803412514 18 19.64541127233426 25.37677926736726 33.10828523466783 21.86952422563065 19 21.213236139535955 27.256421459286685 32.34989767056822 19.180444730609146 20 22.244348885873936 24.595396325338605 33.120702738749515 20.039552050037944 21 22.985719870306067 25.73228781015016 31.77685285257663 19.50513946696714 22 22.213994987007613 25.424609653459658 31.66969439142732 20.691700968105412 23 22.862004737047855 25.002874422241128 32.07303332030262 20.062087520408397 24 21.593579690482212 25.616391105387816 31.519764527330008 21.270264676799965 25 21.646544785558238 26.257330113832356 32.374841899505576 19.72128320110383 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4594.0 1 4594.0 2 2826.0 3 1058.0 4 1058.0 5 1058.0 6 1472.0 7 1886.0 8 1886.0 9 1886.0 10 1659.5 11 1433.0 12 1433.0 13 1433.0 14 1110.5 15 788.0 16 788.0 17 788.0 18 1659.0 19 2530.0 20 2530.0 21 2530.0 22 4163.5 23 5797.0 24 5797.0 25 5797.0 26 8502.5 27 11208.0 28 11208.0 29 11208.0 30 12539.5 31 13871.0 32 13871.0 33 13871.0 34 14883.5 35 15896.0 36 15896.0 37 15896.0 38 16008.5 39 16121.0 40 16121.0 41 16121.0 42 20179.0 43 24237.0 44 24237.0 45 24237.0 46 26275.0 47 28313.0 48 28313.0 49 28313.0 50 28246.0 51 28179.0 52 28179.0 53 28179.0 54 25614.5 55 23050.0 56 23050.0 57 23050.0 58 19531.5 59 16013.0 60 16013.0 61 16013.0 62 13843.5 63 11674.0 64 11674.0 65 11674.0 66 8810.5 67 5947.0 68 5947.0 69 5947.0 70 4490.0 71 3033.0 72 3033.0 73 3033.0 74 2130.0 75 1227.0 76 1227.0 77 1227.0 78 816.5 79 406.0 80 406.0 81 406.0 82 261.0 83 116.0 84 116.0 85 116.0 86 80.0 87 44.0 88 44.0 89 44.0 90 27.5 91 11.0 92 11.0 93 11.0 94 6.5 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4323131050658817 2 0.0 3 0.0 4 0.0 5 0.027594453514843513 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.004599075585807253 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 217435.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.846976914386254 #Duplication Level Percentage of deduplicated Percentage of total 1 79.97471887074904 41.464474030262565 2 13.776505660945551 14.285403419279112 3 3.227281081787186 5.0197430323096714 4 1.064822040953484 2.208312151009798 5 0.4568523897357439 1.1843207651955652 6 0.25115465989165137 0.7812965912005787 7 0.178668783719084 0.6484405413363373 8 0.12605783414492971 0.5228574093431763 9 0.08933185644335713 0.41684280288642034 >10 0.6491438649789172 7.204164982935027 >50 0.10719806433967827 3.8087023233337876 >100 0.08238369759438237 8.294644513916225 >500 0.00694802268868285 2.2931465413829693 >1k 0.007940597358494686 9.239486760320878 >5k 9.925746698118357E-4 2.628164135287887 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5107 2.348747901671764 No Hit TATCAACGCAGAGTACTTTTTTTTT 3823 1.7582265964541128 No Hit GGTATCAACGCAGAGTACTTTTTTT 3745 1.7223538068848159 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 2278 1.0476694184468922 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 2246 1.032952376572309 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1966 0.9041782601697058 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1519 0.6985995814841217 No Hit GTACATAAGCAGTGGTATCAACGCA 1320 0.6070779773265573 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1057 0.4861222894198266 No Hit GTGGTATCAACGCAGAGTACATGGG 772 0.35504863522431995 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 671 0.3085979718076667 No Hit GTGGTATCAACGCAGAGTACTTTTT 662 0.30445880378044016 No Hit GTACTGGTTCACTATCGGTCAGTCA 629 0.2892818543472762 No Hit GGTATCAACGCAGAGTACATGGGGG 610 0.2805436107342424 No Hit ATCAACGCAGAGTACTTTTTTTTTT 581 0.2672062915354014 No Hit GCTTATGTACTCTGCGTTGATACCA 531 0.24421091360636513 No Hit ATACAGGGTGACAGCCCCGTACACA 477 0.21937590544300595 No Hit CTTATGTACTCTGCGTTGATACCAC 411 0.1890220065766781 No Hit GGATACCACGTGTCCCGCCCTACTC 382 0.17568468737783705 No Hit GGTATCAACGCAGAGAAAAAAAAAA 368 0.1692459815577069 No Hit GAGTACATAAGCAGTGGTATCAACG 359 0.16510681353048037 No Hit GGTATCAACGCAGAAAAAAAAAAAA 356 0.1637270908547382 No Hit GTATCAACGCAGAGTACATGGGGGT 347 0.15958792282751166 No Hit ATCATTAACTGAATCCATAGGTTAA 340 0.1563685699174466 No Hit TATGTACTCTGCGTTGATACCACTG 312 0.14349115827718628 No Hit GTACATGGGGGTTAAGCGACTAAGC 302 0.13889208269137904 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 299 0.13751236001563685 No Hit AGCGTACACGGTGGATGCCCTGGCA 292 0.13429300710557177 No Hit GTATCAACGCAGAGAAAAAAAAAAA 280 0.12877411640260308 No Hit CATAAGCAGTGGTATCAACGCAGAG 274 0.12601467105111872 No Hit GGTATCAACGCAGAGTACATGGGGA 271 0.12463494837537654 No Hit GTTAATGATAGTGTGTCGAAACACA 267 0.12279531814105366 No Hit GGTTAATGAGGCGAACCGGGGGAAC 252 0.11589670476234276 No Hit CAACGCAGAGTACTTTTTTTTTTTT 243 0.11175753673511625 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 242 0.11129762917653553 No Hit GTTCACTATCGGTCAGTCAGGAGTA 238 0.10945799894221261 No Hit TATTCAGACAGGATACCACGTGTCC 237 0.10899809138363188 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 233 0.10715846114930899 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 230 0.10577873847356682 No Hit GTATTTAGCCTTGGAGGATGGTCCC 226 0.10393910823924392 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 220 0.10117966288775955 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCCCA 25 0.006025873 18.99448 9 GCCCCGT 110 2.7030183E-9 12.950782 14 CCCGTAC 110 2.7030183E-9 12.950782 16 CCGTACA 110 2.7030183E-9 12.950782 17 AGGGTGA 115 5.2023097E-9 12.393404 5 CAGGGTG 115 5.2023097E-9 12.393404 4 CCCCGTA 115 5.2386895E-9 12.387704 15 TACTGGT 180 0.0 12.140946 2 GTGCAGA 55 0.0030605288 12.087396 11 TGGTTCA 175 0.0 11.944881 5 ACCGGGG 65 7.9942885E-4 11.688911 15 GTACTGG 180 0.0 11.645233 1 ACAGCCC 115 6.9772796E-8 11.561857 11 GTCAGTC 165 7.2759576E-12 11.511806 18 AGCCCCG 125 1.8026185E-8 11.3966875 13 TCGGTCA 175 1.8189894E-12 11.3966875 15 ACAGGGT 120 1.2499913E-7 11.085212 3 ACTGGTT 190 0.0 11.001865 3 ACTATCG 190 0.0 10.996804 11 GGTTCAC 190 0.0 10.996804 6 >>END_MODULE