FastQCFastQC Report
Thu 26 May 2016
SRR1512191_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512191_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24312
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT940.38664034221783483No Hit
TATCAACGCAGAGTACTTTTTTTTT610.2509049029285949No Hit
GGTATCAACGCAGAGTACTTTTTTT590.242678512668641No Hit
CTGTAGGACGTGGAATATGGCAAGA490.20154656136887134No Hit
GTCCTACAGTGGACATTTCTAAATT450.18509378084896347No Hit
CTTTAGGACGTGAAATATGGCGAGG440.1809805857189865No Hit
GTCCTAAAGTGTGTATTTCTCATTT430.17686739058900955No Hit
GTCCTACAGTGTGCATTTCTCATTT270.11105626850937808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG250.00616123718.83950419
TTAGGAC250.00616123718.8395043
TAGGACG350.00221050216.1481484
TATGGCG508.778831E-515.07160416
AGGACGT400.00537838214.1296295
GGACGTG400.00537838214.1296296
ATGGCGA551.9755676E-413.70145917
TGGCGAG551.9755676E-413.70145918
ATATGGC604.125901E-412.5596715
AATATGG550.00311352311.98877714
Produced by FastQC (version 0.11.5)