FastQCFastQC Report
Thu 26 May 2016
SRR1512191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512191_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24312
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1100.45245146429746624No Hit
TATCAACGCAGAGTACTTTTTTTTT850.3496215860480421No Hit
GGTATCAACGCAGAGTACTTTTTTT740.30437643961829547No Hit
CTGTAGGACGTGGAATATGGCAAGA560.23033892727871008No Hit
CTTTAGGACGTGAAATATGGCGAGG440.1809805857189865No Hit
GTCCTACAGTGGACATTTCTAAATT430.17686739058900955No Hit
GTCCTAAAGTGTGTATTTCTCATTT400.16452780519907864No Hit
ACGCAGAGTACTTTTTTTTTTTTTT360.14807502467917077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG600.00560316111.0821331
TTTTTTT1655.98443E-46.92258719
Produced by FastQC (version 0.11.5)