FastQCFastQC Report
Thu 26 May 2016
SRR1512189_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512189_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2795003
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66480.2378530541827683No Hit
GGTATCAACGCAGAGTACTTTTTTT46150.1651161018431823No Hit
TATCAACGCAGAGTACTTTTTTTTT43040.15398910126393425No Hit
GTCCTACAGTGGACATTTCTAAATT35810.12812150827745086No Hit
CTGTAGGACGTGGAATATGGCAAGA30220.108121529744333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG402.9374668E-416.4913527
TAGACCG500.001594110513.1930824
GCGATAC1103.103196E-912.85017414
CGATACT1103.103196E-912.85017415
TAGGACC6650.012.045244
TGCGATA1252.0665539E-811.30815313
AGTACCG2200.011.1370165
GGTATCA19400.011.0458981
GTAGGAC19500.010.7767663
TGTAGGA19450.010.7551822
GGCGAGG9050.010.72600419
GGACGTG21300.010.6624176
TATGTCG1159.792566E-710.65260816
TAGGACA5700.010.5809184
AGGACGT21700.010.5527295
TCCAACG2800.010.43405118
TAGGACG22450.010.4100684
GTCCTAA10050.010.25507451
GTCCTAC18350.010.2321121
ATAGGAC3500.010.2313693
Produced by FastQC (version 0.11.5)