FastQCFastQC Report
Thu 26 May 2016
SRR1512189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2795003
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT90040.3221463447445316No Hit
GGTATCAACGCAGAGTACTTTTTTT62950.22522337185326816No Hit
TATCAACGCAGAGTACTTTTTTTTT62800.22468669979960665No Hit
GTCCTACAGTGGACATTTCTAAATT38850.13899806189832353No Hit
CTGTAGGACGTGGAATATGGCAAGA34790.12447213831255279No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30300.10840775483961915No Hit
GTCCTAAAGTGTGTATTTCTCATTT28480.10189613392185984No Hit
CTTTAGGACGTGAAATATGGCGAGG28210.10093012422526916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21550.011.59415151
GTCGTAG855.1225856E-511.2193061
TAGGACC8050.011.2112544
GGCGAGG8550.011.10879919
TGTAGGA20700.010.4638382
AGGACGT20850.010.297435
ATAGGAC3700.010.2703383
GTAGGAC20100.010.20902253
GGACGTG20700.010.1855566
GATATAC7800.010.1477181
GTCCTAC19300.010.0799361
TAGGACG22100.010.058894
CCCGGTC856.613434E-410.0560118
GACGTGG10300.010.0505867
AATCCCG3250.09.93639419
TGGCGAG19900.09.735970518
GGAGCGT2251.8189894E-129.7083956
TCCAACG2251.8189894E-129.70839518
TAGGACT4350.09.6092594
TTAGGAC12850.09.5370273
Produced by FastQC (version 0.11.5)