FastQCFastQC Report
Thu 26 May 2016
SRR1512188_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512188_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2956039
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70160.23734463584546753No Hit
GGTATCAACGCAGAGTACTTTTTTT42650.14428091104346052No Hit
TATCAACGCAGAGTACTTTTTTTTT41310.1397478179415089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1553.601599E-1010.94506318
GGTATCA21850.010.7780141
AGGACCG1356.401024E-810.4715975
TACTCCG1357.192066E-79.773495
GTATCAA40950.09.6346351
GTCCTAC6000.09.5151371
GGTCTAC2800.09.4665891
TTAGGAC4200.09.4244373
GTAGGAC5800.09.2619473
TAGGACT3650.09.0371314
TCTACAC4850.08.9386413
GGACCGA950.00196191378.9284126
GTAGCAC2750.08.9103773
TAGGACC4550.08.90661054
AGAACCG1701.9056279E-78.8700585
TAAGACT3200.08.8354094
CGTACAC2253.074092E-108.7961413
TTATACT3650.08.7789284
AGCGTAC1404.5551235E-58.738391
GACAGTC3150.08.6764667
Produced by FastQC (version 0.11.5)