FastQCFastQC Report
Thu 26 May 2016
SRR1512188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512188_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2956039
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93270.31552357732763336No Hit
TATCAACGCAGAGTACTTTTTTTTT60000.20297431799783427No Hit
GGTATCAACGCAGAGTACTTTTTTT58800.19891483163787757No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31070.10510686766987852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG2000.012.34747119
GGTATCA26500.011.552681
GCGTACA1251.8337232E-811.4001722
TATACCG700.001491903310.8573075
TAGGACC3550.010.4367784
CGCTGTA856.5957726E-410.0589762
CGTACAC2300.09.9131943
TAGATTG1651.0229996E-89.7880275
ACTGTTC6850.09.5665748
AGCGTAC1304.0353007E-69.5372591
CTACGAC900.00111398439.5001443
CCGTATA800.00452827949.49725159
ACCGTAT1606.6711436E-89.49725158
CGATCAA800.00452827949.497251510
TCTAGGA3750.09.1201382
TAGGACG5150.09.0389724
GATATAC4750.09.0352971
GATCGTC950.00182928098.99739557
ACCGTGC1804.2420652E-88.9696268
GTATTAG2252.419256E-108.9014431
Produced by FastQC (version 0.11.5)