##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512188_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2956039 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.895752728566844 31.0 31.0 34.0 28.0 34.0 2 31.08616022995637 31.0 31.0 34.0 27.0 34.0 3 31.17527881059756 31.0 31.0 34.0 27.0 34.0 4 34.78864791702681 37.0 35.0 37.0 32.0 37.0 5 34.3982880469439 37.0 35.0 37.0 30.0 37.0 6 34.32600686256169 37.0 35.0 37.0 30.0 37.0 7 34.152927278699636 37.0 35.0 37.0 30.0 37.0 8 34.06641860949737 37.0 35.0 37.0 30.0 37.0 9 35.55930148418204 39.0 35.0 39.0 30.0 39.0 10 35.378926665040616 39.0 35.0 39.0 30.0 39.0 11 35.368944726372014 39.0 35.0 39.0 30.0 39.0 12 35.17062291803322 38.0 35.0 39.0 29.0 39.0 13 35.148645873752 38.0 35.0 39.0 29.0 39.0 14 36.16368457926299 39.0 36.0 41.0 29.0 41.0 15 36.0719709719662 39.0 36.0 41.0 28.0 41.0 16 35.96905013770116 39.0 36.0 41.0 27.0 41.0 17 35.89418813486561 39.0 36.0 41.0 27.0 41.0 18 35.82208895078854 39.0 36.0 41.0 27.0 41.0 19 35.837332998651235 39.0 36.0 41.0 27.0 41.0 20 35.70172281218211 39.0 36.0 41.0 27.0 41.0 21 35.60336348742354 39.0 35.0 41.0 27.0 41.0 22 35.690930329403635 39.0 36.0 41.0 27.0 41.0 23 35.582095500093196 39.0 36.0 41.0 27.0 41.0 24 35.442637935426426 39.0 36.0 41.0 27.0 41.0 25 34.97163569222192 39.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17281943655584797 1101 2 0.19977466489176265 1101 3 0.22220079991826225 1101 4 0.19590941202795165 1101 5 0.1705645382602512 1101 6 0.17048572472376833 1101 7 0.07890165218744727 1101 8 -0.09748223323127547 1101 9 -0.003163860014907982 1101 10 0.07392452024516416 1101 11 0.14304542597775338 1101 12 0.371831872857328 1101 13 0.1936331038373993 1101 14 0.11185740951951573 1101 15 0.0014768533390636662 1101 16 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-0.255199501624233 2313 8 -0.17272170357920658 2313 9 -0.28912648873708235 2313 10 -0.26087364046157546 2313 11 -0.15247177692076974 2313 12 -0.009451479654366324 2313 13 -0.11700969750831547 2313 14 -0.21971137737748592 2313 15 -0.18782020331607185 2313 16 -0.14710822984061167 2313 17 -0.16225338166469072 2313 18 -0.3801926654876553 2313 19 -0.10912146812304968 2313 20 -0.30037427199141575 2313 21 -0.31083004845800843 2313 22 -0.19997662703875818 2313 23 -0.14116627099918588 2313 24 -0.10168929770561164 2313 25 -0.02754892574583323 2314 1 -0.33035472197700244 2314 2 -0.4146423263981234 2314 3 -0.40531287802632576 2314 4 -0.327771815266658 2314 5 -0.4155051864300816 2314 6 -0.34378420130590825 2314 7 -0.32405956810406167 2314 8 -0.34866904112727326 2314 9 -0.46432243663328165 2314 10 -0.3930783685367274 2314 11 -0.4444724100619908 2314 12 -0.3237075852772193 2314 13 -0.5791782061814175 2314 14 -0.551813313113648 2314 15 -0.33482572467993066 2314 16 -0.36481141696177133 2314 17 -0.35657429115342865 2314 18 -0.4216958172273948 2314 19 -0.4548696455143215 2314 20 -0.4337013359852335 2314 21 -0.4078494430632702 2314 22 -0.5068584996470236 2314 23 -0.36659731912723004 2314 24 -0.31553795833261233 2314 25 -0.5014080145811377 2315 1 -0.49296676008267326 2315 2 -0.49342540967114346 2315 3 -0.4236470264193528 2315 4 -0.5020776778280336 2315 5 -0.5479638629035506 2315 6 -0.706351443730739 2315 7 -0.7103452432669073 2315 8 -0.6672989583824176 2315 9 -0.7501957567952502 2315 10 -0.7869757883837565 2315 11 -0.7570864043109253 2315 12 -0.924364458186588 2315 13 -1.1468791509986218 2315 14 -1.1524435824745751 2315 15 -1.0028273287174088 2315 16 -1.2628359078754912 2315 17 -1.1262929977514986 2315 18 -1.2042982702310567 2315 19 -1.2048021585715816 2315 20 -1.1951758767805956 2315 21 -1.1275569996447175 2315 22 -1.1353415105427374 2315 23 -1.269980077680465 2315 24 -0.9977671792007499 2315 25 -0.9651902175896865 2316 1 -0.7565741327715969 2316 2 -0.7550527397055475 2316 3 -0.7147375000684697 2316 4 -0.6771680474023825 2316 5 -0.7675315734240158 2316 6 -0.85774211258056 2316 7 -0.8300287067138754 2316 8 -0.7437418763969106 2316 9 -1.072910337388052 2316 10 -1.0605771626547806 2316 11 -0.9791293194210127 2316 12 -1.1378875625836642 2316 13 -1.0543651523370698 2316 14 -1.389599727303093 2316 15 -1.2386844550355391 2316 16 -1.2479151963106432 2316 17 -1.2808858818610176 2316 18 -1.2015576346407926 2316 19 -1.1938313467036679 2316 20 -1.1298618997407814 2316 21 -1.2001306960779772 2316 22 -1.1816362268926 2316 23 -1.0722419491626454 2316 24 -1.1744016270619113 2316 25 -1.1271274322664837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41383.0 3 2.0 4 723.0 5 6933.0 6 8881.0 7 6971.0 8 5823.0 9 5373.0 10 5132.0 11 4969.0 12 4638.0 13 4755.0 14 4607.0 15 4672.0 16 4668.0 17 4946.0 18 5369.0 19 5939.0 20 7422.0 21 9535.0 22 12250.0 23 15375.0 24 18971.0 25 22656.0 26 26467.0 27 30496.0 28 35682.0 29 44514.0 30 56520.0 31 72618.0 32 93136.0 33 122239.0 34 157151.0 35 208893.0 36 297627.0 37 509362.0 38 1089341.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.16008813200506 18.53826017421222 13.720417995180323 24.581233698602404 2 16.046506828901784 19.8031216773527 38.801314867631994 25.349056626113526 3 19.559045059960305 23.29996322781939 29.32089190974815 27.82009980247216 4 13.595862571501932 16.2699138949114 36.13639738853243 33.99782614505425 5 14.450787832561174 36.79753161702028 34.453982274232665 14.297698276185876 6 33.83822067300194 35.58806226846127 16.81067130711063 13.763045751426148 7 29.57254623501246 31.17310021958438 20.458491921114707 18.79586162428845 8 26.69985071238911 35.597534403301175 19.091561376558293 18.61105350775142 9 26.316060106108207 15.87739539295659 19.31297252844093 38.49357197249427 10 15.749115623981957 28.85719708028209 32.39892978407931 22.994757511656648 11 35.69056429905018 22.581637116425053 22.346254565653563 19.3815440188712 12 23.599282688760194 24.2924399847228 30.006268523520834 22.10200880299617 13 29.015347903055407 20.925332852509726 25.497836801205935 24.56148244322893 14 22.155086587152606 20.706052930966067 26.658139490040554 30.48072099184077 15 24.216764393162606 28.543466442763442 23.11265176136039 24.127117402713562 16 23.834462265213684 27.755959917984846 25.13661017327579 23.27296764352568 17 23.005041543768538 27.407047065346568 26.36636390791867 23.221547482966226 18 22.877776646383893 27.074981081102113 27.348590461763187 22.698651810750807 19 23.95310075408342 26.58124605257238 26.904347337771934 22.561305855572268 20 24.023059235686674 26.286189052309528 27.118417585153647 22.57233412685015 21 24.27106002322703 25.914407759843495 26.78334081519222 23.031191401737257 22 23.95425094188541 25.911701435603522 27.0286352784926 23.105412344018465 23 23.899380217919994 25.80700051656964 26.84460523017457 23.4490140353358 24 23.49309329139433 26.025806831371305 27.04335091654745 23.437748960686918 25 23.792237476136037 25.938581448039578 26.98610908591432 23.283071989910063 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 335.0 1 335.0 2 525.0 3 715.0 4 715.0 5 715.0 6 1772.0 7 2829.0 8 2829.0 9 2829.0 10 4100.0 11 5371.0 12 5371.0 13 5371.0 14 8174.0 15 10977.0 16 10977.0 17 10977.0 18 18897.5 19 26818.0 20 26818.0 21 26818.0 22 42297.0 23 57776.0 24 57776.0 25 57776.0 26 81840.5 27 105905.0 28 105905.0 29 105905.0 30 135187.0 31 164469.0 32 164469.0 33 164469.0 34 193333.0 35 222197.0 36 222197.0 37 222197.0 38 254249.5 39 286302.0 40 286302.0 41 286302.0 42 317367.5 43 348433.0 44 348433.0 45 348433.0 46 367728.5 47 387024.0 48 387024.0 49 387024.0 50 392264.5 51 397505.0 52 397505.0 53 397505.0 54 369826.0 55 342147.0 56 342147.0 57 342147.0 58 299593.5 59 257040.0 60 257040.0 61 257040.0 62 213725.5 63 170411.0 64 170411.0 65 170411.0 66 133280.5 67 96150.0 68 96150.0 69 96150.0 70 70707.0 71 45264.0 72 45264.0 73 45264.0 74 31873.0 75 18482.0 76 18482.0 77 18482.0 78 12655.0 79 6828.0 80 6828.0 81 6828.0 82 4530.0 83 2232.0 84 2232.0 85 2232.0 86 1431.0 87 630.0 88 630.0 89 630.0 90 404.0 91 178.0 92 178.0 93 178.0 94 98.0 95 18.0 96 18.0 97 18.0 98 10.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41518396746457 2 0.0 3 0.0 4 0.0 5 0.030649122017672975 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.007510049765919868 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2956039.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.01073402762793 #Duplication Level Percentage of deduplicated Percentage of total 1 80.77850179906495 54.93805201006471 2 12.23033603298778 16.635882620160924 3 3.36965598908877 6.875183317155596 4 1.3387888318031334 3.642080446356865 5 0.6869869358835302 2.3361242888414933 6 0.4052886582971509 1.653838748631703 7 0.26395333037032465 1.2566161823265931 8 0.17154116625640822 0.9333312506442948 9 0.13602059366597768 0.8325774376287176 >10 0.5824746548233045 6.722536326793284 >50 0.023632872903979807 1.0924544288477713 >100 0.011227367694523847 1.280700352593794 >500 8.953690278195032E-4 0.45332724683046727 >1k 5.471699611428444E-4 0.6298542297119206 >5k 1.4922817122077576E-4 0.7174411134117897 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9327 0.31552357732763336 No Hit TATCAACGCAGAGTACTTTTTTTTT 6000 0.20297431799783427 No Hit GGTATCAACGCAGAGTACTTTTTTT 5880 0.19891483163787757 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3107 0.10510686766987852 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.3829052999639045E-5 2 0.0 6.765810599927809E-5 0.0 0.0 3.3829052999639045E-5 3 0.0 6.765810599927809E-5 0.0 0.0 3.3829052999639045E-5 4 0.0 6.765810599927809E-5 0.0 0.0 3.3829052999639045E-5 5 0.0 1.3531621199855618E-4 0.0 0.0 3.3829052999639045E-5 6 0.0 1.3531621199855618E-4 0.0 3.3829052999639045E-5 6.765810599927809E-5 7 0.0 1.3531621199855618E-4 0.0 3.3829052999639045E-5 6.765810599927809E-5 8 0.0 1.3531621199855618E-4 0.0 3.3829052999639045E-5 6.765810599927809E-5 9 0.0 1.3531621199855618E-4 0.0 6.765810599927809E-5 6.765810599927809E-5 10 3.3829052999639045E-5 1.3531621199855618E-4 0.0 6.765810599927809E-5 6.765810599927809E-5 11 3.3829052999639045E-5 1.3531621199855618E-4 0.0 1.0148715899891713E-4 6.765810599927809E-5 12 3.3829052999639045E-5 1.3531621199855618E-4 0.0 1.0148715899891713E-4 6.765810599927809E-5 13 3.3829052999639045E-5 1.6914526499819523E-4 0.0 1.0148715899891713E-4 1.6914526499819523E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCCCG 200 0.0 12.347471 19 GGTATCA 2650 0.0 11.55268 1 GCGTACA 125 1.8337232E-8 11.400172 2 TATACCG 70 0.0014919033 10.857307 5 TAGGACC 355 0.0 10.436778 4 CGCTGTA 85 6.5957726E-4 10.058976 2 CGTACAC 230 0.0 9.913194 3 TAGATTG 165 1.0229996E-8 9.788027 5 ACTGTTC 685 0.0 9.566574 8 AGCGTAC 130 4.0353007E-6 9.537259 1 CTACGAC 90 0.0011139843 9.500144 3 CCGTATA 80 0.0045282794 9.4972515 9 ACCGTAT 160 6.6711436E-8 9.4972515 8 CGATCAA 80 0.0045282794 9.4972515 10 TCTAGGA 375 0.0 9.120138 2 TAGGACG 515 0.0 9.038972 4 GATATAC 475 0.0 9.035297 1 GATCGTC 95 0.0018292809 8.9973955 7 ACCGTGC 180 4.2420652E-8 8.969626 8 GTATTAG 225 2.419256E-10 8.901443 1 >>END_MODULE