FastQCFastQC Report
Thu 26 May 2016
SRR1512187_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512187_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2732609
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT63730.23322033997545938No Hit
GTATCAACGCAGAGTACTTTTTTTT63230.23139058679818447No Hit
CTGTAGGACGTGGAATATGGCAAGA53970.1975035579550532No Hit
GTCCTAAAGTGTGTATTTCTCATTT49460.1809991842960336No Hit
CTTTAGGACGTGAAATATGGCGAGG42880.15691963248309582No Hit
GGTATCAACGCAGAGTACTTTTTTT39380.1441113602421715No Hit
TATCAACGCAGAGTACTTTTTTTTT38480.14081780452307666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3150.013.76409718
TTATCGC550.003258287711.99488453
TAGGACC9900.011.42374
GTCCTAG4500.011.3238021
CCAACGA2450.011.15661319
AATCCCG4400.011.13912719
TAGTACG600.006236850710.995314
GGCGAGG12550.010.81481919
AATGTCC7400.010.6979458
GTCTAAG2500.010.5990781
TTAGGAC18150.010.5409593
GTCCTAC30400.010.5266661
GATATAC9900.010.5002531
GTCCTAA14100.010.408261
TGTAGGA29650.010.3117262
AGGACGT29350.010.2754915
GGACGTG29100.010.1046746
TCCTACA34200.09.9828262
AGTGCGA857.0990267E-49.97875510
ATTTAGA8100.09.9397811
Produced by FastQC (version 0.11.5)