FastQCFastQC Report
Thu 26 May 2016
SRR1512187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512187_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2732609
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82710.3026777705848147No Hit
GTCCTACAGTGGACATTTCTAAATT62750.22963402374800052No Hit
CTGTAGGACGTGGAATATGGCAAGA58600.21444707237661884No Hit
TATCAACGCAGAGTACTTTTTTTTT57070.20884802765415764No Hit
GGTATCAACGCAGAGTACTTTTTTT52650.19267300956704744No Hit
GTCCTAAAGTGTGTATTTCTCATTT48600.17785200883112073No Hit
CTTTAGGACGTGAAATATGGCGAGG48480.17741286806857476No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28410.10396657553276008No Hit
CTGAAGGACCTGGAATATGGCGAGA27440.10041685436884677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA2700.014.07097119
TCCAACG3700.013.09122818
TAAGACG604.0944575E-412.6664224
TAGGACC9650.011.8132444
CGTTTCC2750.011.39706914
CCGTGCG600.005888853711.0804839
CCGTTTC2950.010.94633813
GGCGAGG14250.010.93092219
TGTAGGA32900.010.8858082
CCCGTTT3250.010.52037112
GATATAC10400.010.4515171
GTAGGAC32350.010.3073753
CTGTAGG33100.010.28366851
CGTCAAT750.002569707610.1703661
ATCCCGT3300.010.07316810
AACCGTG856.611962E-410.0562387
AGGACGT30900.010.022465
GTATAGA4200.09.9887511
TGGACCG1054.108174E-59.9521895
GCGCCAC1252.5801291E-69.8774613
Produced by FastQC (version 0.11.5)