FastQCFastQC Report
Thu 26 May 2016
SRR1512186_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512186_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770950
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70850.25568848228946756No Hit
GGTATCAACGCAGAGTACTTTTTTT43240.15604756491455998No Hit
TATCAACGCAGAGTACTTTTTTTTT41750.15067034771468268No Hit
GTCCTACAGTGGACATTTCTAAATT34220.12349555206698064No Hit
CTGTAGGACGTGGAATATGGCAAGA30310.10938486800555766No Hit
GTCCTAAAGTGTGTATTTCTCATTT28280.10205886067955033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3200.013.25302818
GTCAACG851.7759739E-613.2019042
CGGTAGG705.603927E-513.1161771
CCAACGA2000.012.72290819
CCGCGCG658.589818E-411.5981869
GGCGAGG10300.010.6138619
GTCCTAA10400.010.5938361
TGTAGGA18200.010.5938352
GATATAC9700.010.3066411
AGGACGT19300.010.2535775
GTATTAA2203.6379788E-1210.201471
TAGGACC5750.010.1610184
CTAGTAC951.7845882E-49.91953
GGTATCA19750.09.8657261
GGACGTG19700.09.8062076
AGACCGT1252.8514369E-69.8004676
GTAGGAC19450.09.78693
TTAGGAC13600.09.7699793
TAGGACG21200.09.6902284
GTATCAA37600.09.6859711
Produced by FastQC (version 0.11.5)