FastQCFastQC Report
Thu 26 May 2016
SRR1512186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770950
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93900.33887294971038817No Hit
TATCAACGCAGAGTACTTTTTTTTT59910.21620743788231472No Hit
GGTATCAACGCAGAGTACTTTTTTT59000.21292336563272524No Hit
GTCCTACAGTGGACATTTCTAAATT38040.1372814377740486No Hit
CTGTAGGACGTGGAATATGGCAAGA35430.12786228549775347No Hit
GTCCTAAAGTGTGTATTTCTCATTT30160.10884353741496598No Hit
CTTTAGGACGTGAAATATGGCGAGG29500.10646168281636262No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29430.1062090618740865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3200.013.05870318
TAGGACC5550.011.6398284
CCAACGA2300.011.56269219
ACACCGC1104.991671E-711.22400956
CGCGCCT600.005890356411.08011212
CGTTTCC2650.010.39320514
GGCGAGG9150.010.38024619
TCCCGTT2750.010.36062411
CCGTTTC2800.010.17561313
GTAGGAC18250.010.1508483
TTAGGAC13100.010.0077953
GGTATCA22200.09.9671281
ATCCCGT2700.09.84898910
CCCGTTT3100.09.80360212
TGTAGGA20050.09.76075652
GTCCTAA10750.09.7593131
GTCCTAC18600.09.7426191
TAGGACG20400.09.6864314
GGACGTG18950.09.672656
AGGACGT19500.09.5488725
Produced by FastQC (version 0.11.5)