FastQCFastQC Report
Thu 26 May 2016
SRR1512185_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512185_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3156980
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81650.25863325076497157No Hit
GTCCTACAGTGGACATTTCTAAATT64650.2047843191911257No Hit
CTGTAGGACGTGGAATATGGCAAGA60220.1907519211398235No Hit
GTCCTAAAGTGTGTATTTCTCATTT52560.1664882260894906No Hit
TATCAACGCAGAGTACTTTTTTTTT48250.15283593814341556No Hit
GGTATCAACGCAGAGTACTTTTTTT48140.15248750388029067No Hit
CTTTAGGACGTGAAATATGGCGAGG46730.14802121014387165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG654.3745758E-412.5674671
TCCAACG3800.011.40757118
TAGGACC10000.011.3069434
CAAGTCG1950.011.1138386
CGGTAGG1251.0193144E-610.619511
GGTATCA21700.010.5404551
TGTAGGA33400.010.5167862
CCAACGA3000.010.36601119
GTCCTAC34050.010.3460141
GTATTAT4050.010.3371291
CTGTAGG33600.010.1502871
GTAGGAC35100.010.1204123
AAGTCGT2151.8189894E-1210.0799927
GGCGAGG14500.010.00856219
CACCTTT9050.09.99572114
GATATAC11500.09.9446911
GTCCTAA16350.09.9300281
ATTTCTA26200.09.89076115
AGGACGT34300.09.8070415
GACGTGG18550.09.8035697
Produced by FastQC (version 0.11.5)