FastQCFastQC Report
Thu 26 May 2016
SRR1512185_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512185_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3156980
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT112590.3566383062293711No Hit
GTCCTACAGTGGACATTTCTAAATT73730.23354598381997985No Hit
TATCAACGCAGAGTACTTTTTTTTT71330.2259437817154369No Hit
GGTATCAACGCAGAGTACTTTTTTT69660.2206539160843591No Hit
CTGTAGGACGTGGAATATGGCAAGA67980.21533237461117904No Hit
GTCCTAAAGTGTGTATTTCTCATTT60340.19113203124505065No Hit
CTTTAGGACGTGAAATATGGCGAGG57690.18273793308795114No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35600.11276599788405375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG400.00529107814.2457839
CCAACGA2550.013.40885719
TCCAACG3950.012.983518
GGCGAGG15950.011.90964319
TAGGACC10800.011.7876574
CCGCTCA1053.4816258E-610.8539319
GGACGTG37300.010.7448096
AGGACGT38100.010.6724775
TGTAGGA35200.010.6070912
GTCCTAA20700.010.5964031
GGTATCA23900.010.5742341
TTAGGAC22750.010.3983483
GTAGGAC34850.010.3592233
GACGTGA20600.010.327047
GACGTGG18150.010.3082447
TGGCGAG33350.010.19488318
TAGGACG41200.010.1922834
GTCTAGG2400.09.9341281
CTGTAGG36400.09.8249611
TTTAGGA25150.09.7838142
Produced by FastQC (version 0.11.5)