##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512185_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3156980 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.390489645167218 31.0 31.0 34.0 28.0 34.0 2 31.5887249840037 33.0 31.0 34.0 28.0 34.0 3 31.680366046031335 34.0 31.0 34.0 28.0 34.0 4 35.25792054431767 37.0 35.0 37.0 32.0 37.0 5 34.950901811224654 37.0 35.0 37.0 32.0 37.0 6 34.91003585705326 37.0 35.0 37.0 32.0 37.0 7 34.77297543855203 37.0 35.0 37.0 32.0 37.0 8 34.69864047285697 37.0 35.0 37.0 32.0 37.0 9 36.275485115521796 39.0 37.0 39.0 32.0 39.0 10 36.119997592636 39.0 37.0 39.0 32.0 39.0 11 36.11068679560846 39.0 37.0 39.0 32.0 39.0 12 35.967788202649366 39.0 35.0 39.0 31.0 39.0 13 35.947561910433386 39.0 35.0 39.0 31.0 39.0 14 37.066231335010045 40.0 37.0 41.0 31.0 41.0 15 37.01081191518477 40.0 37.0 41.0 31.0 41.0 16 36.928468029572564 40.0 37.0 41.0 31.0 41.0 17 36.88064099234078 40.0 37.0 41.0 31.0 41.0 18 36.82801696558103 40.0 37.0 41.0 31.0 41.0 19 36.86201908152728 40.0 37.0 41.0 31.0 41.0 20 36.758489125683404 40.0 37.0 41.0 30.0 41.0 21 36.668710603171384 40.0 36.0 41.0 30.0 41.0 22 36.757737141191896 40.0 37.0 41.0 31.0 41.0 23 36.6708021590254 40.0 37.0 41.0 30.0 41.0 24 36.57335808272463 40.0 37.0 41.0 30.0 41.0 25 36.16583887132639 39.0 36.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13264203136158415 1101 2 0.10350784675896207 1101 3 0.248242630822606 1101 4 0.22110787451020286 1101 5 0.2423190280555616 1101 6 0.2702916630936585 1101 7 0.18102868421110685 1101 8 -0.01075072139169464 1101 9 0.15401276415686738 1101 10 0.244703236653109 1101 11 0.2219620891694234 1101 12 0.29617627046810213 1101 13 0.14415128713314118 1101 14 0.09108113059209444 1101 15 0.06731815044983591 1101 16 0.08750225007091927 1101 17 0.1139496519818124 1101 18 0.2749482975113864 1101 19 0.21016344140509347 1101 20 0.16158583348497757 1101 21 0.13024288314570498 1101 22 0.09226182269411964 1101 23 0.11206634852477748 1101 24 0.16561873378639547 1101 25 0.23613813111849424 1102 1 0.2208765567859139 1102 2 0.25925423951532167 1102 3 0.20647822305349806 1102 4 0.24300829655218337 1102 5 0.28169072413306395 1102 6 0.33887235655593884 1102 7 0.2763105480480945 1102 8 0.38480879726709105 1102 9 0.5526988101131849 1102 10 0.39085193428187637 1102 11 0.5351624779717099 1102 12 0.5653963113203844 1102 13 0.5576857835945077 1102 14 0.47693639226501006 1102 15 0.5851780129339517 1102 16 0.5571682350743288 1102 17 0.5370750720814428 1102 18 0.7008001330483111 1102 19 0.5936727978213625 1102 20 0.7069177469439154 1102 21 0.5919764842023483 1102 22 0.5864790896117356 1102 23 0.5884855279485777 1102 24 0.6005227437673284 1102 25 0.5914956740705293 1103 1 0.2593419871602016 1103 2 0.23806593021112832 1103 3 0.24335367558696674 1103 4 0.28840830024424235 1103 5 0.14053686036042023 1103 6 0.19465100812072222 1103 7 0.27888097234074394 1103 8 0.2936997509734667 1103 9 0.43212851025656107 1103 10 0.33880417546349406 1103 11 0.24417978209949354 1103 12 0.31816415814647314 1103 13 0.4698609594287575 1103 14 0.4543098897171518 1103 15 0.45828027814813765 1103 16 0.5739616282053248 1103 17 0.2910548026843003 1103 18 0.5924279818298288 1103 19 0.6307246486704301 1103 20 0.41309661073365334 1103 21 0.4617842715726397 1103 22 0.4766513104323451 1103 23 0.5626452258562011 1103 24 0.4436786600506011 1103 25 0.48191403207569294 1104 1 0.25850889216782136 1104 2 0.22300135409118127 1104 3 0.2912834921940366 1104 4 0.2566621638938855 1104 5 0.2605157194079979 1104 6 0.2913052942379437 1104 7 0.27780151847322543 1104 8 0.23740471606585345 1104 9 0.34475546251060507 1104 10 0.3020420054668662 1104 11 0.37875345493579005 1104 12 0.40479362605084646 1104 13 0.38146194723668714 1104 14 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>>Per sequence quality scores pass #Quality Count 2 28313.0 3 2.0 4 516.0 5 4714.0 6 6366.0 7 5074.0 8 4149.0 9 3907.0 10 3725.0 11 3686.0 12 3477.0 13 3403.0 14 3345.0 15 3405.0 16 3497.0 17 3755.0 18 4120.0 19 4567.0 20 5601.0 21 7234.0 22 9738.0 23 12490.0 24 15363.0 25 18683.0 26 22653.0 27 25729.0 28 30847.0 29 38460.0 30 50116.0 31 65539.0 32 86001.0 33 114071.0 34 150694.0 35 202469.0 36 294634.0 37 508340.0 38 1408297.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.8548715469086 19.501775057578033 13.8755423787044 24.76781101680897 2 14.338450037694253 20.468960842323995 41.54793505185335 23.6446540681284 3 18.656374129706236 24.61605711787848 30.093918871833207 26.633649880582077 4 12.40451317398273 17.292855830572258 38.260489455112165 32.04214154033285 5 13.04931544699372 37.87361435368614 35.84942368773939 13.227646511580746 6 31.48946144733258 37.429632116769824 18.118106544862496 12.962799891035104 7 27.850065568993156 31.722722348573633 22.103719377379647 18.323492705053564 8 24.75539914728633 37.76989401263233 20.44605920848406 17.028647631597284 9 26.017333020798354 15.796394022135079 20.610266773942186 37.57600618312438 10 15.042762386838055 29.489258721943123 34.093405723191154 21.374573168027673 11 33.905726358735244 22.71395447547973 24.80170922843984 18.578609937345185 12 23.29207026968812 24.680105670609255 31.93856153665845 20.089262523044173 13 28.80354009211335 22.187121869634904 26.089237182370496 22.920100855881252 14 20.63484722741354 22.686428168692867 27.73533566889876 28.943388934994836 15 22.61062787854215 31.555378874747387 23.59828063529069 22.235712611419775 16 21.642994254002243 28.47930617235459 28.356023794892586 21.52167577875058 17 21.237860233514308 28.925143649943934 28.29960912010846 21.5373869964333 18 21.002667105905008 27.66504697527384 30.714005156827096 20.618280761994058 19 23.166855665857877 26.989147856495766 27.981647017085947 21.86234946056041 20 23.09479312507523 27.67626022337804 28.730717331120246 20.498229320426482 21 22.497228363816053 27.015343777914335 27.71005834690115 22.77736951136846 22 22.647815317170206 28.25450272095484 28.04759612034286 21.0500858415321 23 21.967228173761 27.829507947468784 28.45577102167261 21.747492857097605 24 22.72238024947893 26.913569297239764 29.13233533313483 21.231715120146468 25 22.4304252350837 27.526587488136528 28.721773166562553 21.321214110217223 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 522.0 1 522.0 2 746.0 3 970.0 4 970.0 5 970.0 6 2480.0 7 3990.0 8 3990.0 9 3990.0 10 5651.5 11 7313.0 12 7313.0 13 7313.0 14 11246.5 15 15180.0 16 15180.0 17 15180.0 18 27229.0 19 39278.0 20 39278.0 21 39278.0 22 62189.5 23 85101.0 24 85101.0 25 85101.0 26 123280.5 27 161460.0 28 161460.0 29 161460.0 30 209630.0 31 257800.0 32 257800.0 33 257800.0 34 286705.0 35 315610.0 36 315610.0 37 315610.0 38 335367.5 39 355125.0 40 355125.0 41 355125.0 42 376027.0 43 396929.0 44 396929.0 45 396929.0 46 400743.0 47 404557.0 48 404557.0 49 404557.0 50 388432.0 51 372307.0 52 372307.0 53 372307.0 54 329579.5 55 286852.0 56 286852.0 57 286852.0 58 245755.0 59 204658.0 60 204658.0 61 204658.0 62 167065.0 63 129472.0 64 129472.0 65 129472.0 66 99474.0 67 69476.0 68 69476.0 69 69476.0 70 50674.0 71 31872.0 72 31872.0 73 31872.0 74 22141.0 75 12410.0 76 12410.0 77 12410.0 78 8366.0 79 4322.0 80 4322.0 81 4322.0 82 2852.5 83 1383.0 84 1383.0 85 1383.0 86 846.0 87 309.0 88 309.0 89 309.0 90 191.0 91 73.0 92 73.0 93 73.0 94 42.0 95 11.0 96 11.0 97 11.0 98 5.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42508980101235994 2 0.0 3 0.0 4 0.0 5 0.03332298589157993 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0073487953677248505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3156980.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.79762954071306 #Duplication Level Percentage of deduplicated Percentage of total 1 82.2480928719052 56.584738238314955 2 12.035292664835167 16.559992123387822 3 3.094224093734642 6.386258487501134 4 1.0645261832647182 2.9294751197054114 5 0.4975383280562404 1.7114728787959494 6 0.27661357102130246 1.1418214791034373 7 0.17403884122991314 0.8381421807241379 8 0.11418787489962165 0.6284684092308366 9 0.08248772832403621 0.5107464157402825 >10 0.3689086532107413 4.403120653892866 >50 0.022413039152708987 1.0599847337147672 >100 0.017308511572560546 2.4067030130808083 >500 0.0020688815373105535 0.9383932061164015 >1k 0.0019769312399256795 2.277061652913244 >5k 2.7585087068730406E-4 1.2675013767997725 >10k+ 4.597514511455068E-5 0.3561200309781808 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 11259 0.3566383062293711 No Hit GTCCTACAGTGGACATTTCTAAATT 7373 0.23354598381997985 No Hit TATCAACGCAGAGTACTTTTTTTTT 7133 0.2259437817154369 No Hit GGTATCAACGCAGAGTACTTTTTTT 6966 0.2206539160843591 No Hit CTGTAGGACGTGGAATATGGCAAGA 6798 0.21533237461117904 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6034 0.19113203124505065 No Hit CTTTAGGACGTGAAATATGGCGAGG 5769 0.18273793308795114 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3560 0.11276599788405375 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.2670336840904915E-4 0.0 0.0 3.167584210226229E-5 0.0 7 1.2670336840904915E-4 0.0 0.0 6.335168420452458E-5 0.0 8 1.2670336840904915E-4 0.0 0.0 6.335168420452458E-5 0.0 9 1.2670336840904915E-4 0.0 0.0 1.5837921051131145E-4 0.0 10 1.2670336840904915E-4 0.0 0.0 2.21730894715836E-4 0.0 11 1.2670336840904915E-4 0.0 0.0 2.850825789203606E-4 0.0 12 1.2670336840904915E-4 0.0 0.0 3.167584210226229E-4 0.0 13 1.2670336840904915E-4 0.0 0.0 3.167584210226229E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTGCG 40 0.005291078 14.245783 9 CCAACGA 255 0.0 13.408857 19 TCCAACG 395 0.0 12.9835 18 GGCGAGG 1595 0.0 11.909643 19 TAGGACC 1080 0.0 11.787657 4 CCGCTCA 105 3.4816258E-6 10.853931 9 GGACGTG 3730 0.0 10.744809 6 AGGACGT 3810 0.0 10.672477 5 TGTAGGA 3520 0.0 10.607091 2 GTCCTAA 2070 0.0 10.596403 1 GGTATCA 2390 0.0 10.574234 1 TTAGGAC 2275 0.0 10.398348 3 GTAGGAC 3485 0.0 10.359223 3 GACGTGA 2060 0.0 10.32704 7 GACGTGG 1815 0.0 10.308244 7 TGGCGAG 3335 0.0 10.194883 18 TAGGACG 4120 0.0 10.192283 4 GTCTAGG 240 0.0 9.934128 1 CTGTAGG 3640 0.0 9.824961 1 TTTAGGA 2515 0.0 9.783814 2 >>END_MODULE