FastQCFastQC Report
Thu 26 May 2016
SRR1512184_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512184_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371022
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46370.3382148499440563No Hit
TATCAACGCAGAGTACTTTTTTTTT29360.21414681894236562No Hit
GGTATCAACGCAGAGTACTTTTTTT28280.206269483640671No Hit
GTCCTACAGTGGACATTTCTAAATT24640.1797199461423668No Hit
CTGTAGGACGTGGAATATGGCAAGA22480.1639652755389775No Hit
GTCCTAAAGTGTGTATTTCTCATTT21150.1542644829915202No Hit
CTTTAGGACGTGAAATATGGCGAGG19520.1423755417491477No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14120.10298886524067448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG652.7396454E-514.1522452
TAGGACC4250.013.3008554
TCCAACG2050.012.87065318
CCAACGA1451.1277734E-1011.69771219
TGGCGAA658.58778E-411.59773218
GGTATCA12150.011.1884921
CCTAGAC855.7910707E-511.0840443
GAACAGT4850.011.07261856
AGGACCT9100.010.9744235
CGTTTCC1752.3646862E-1110.76814514
AGAACAG5850.010.6293155
GTCATAT1352.3582106E-710.5996241
CAGCTTA804.0719833E-410.5987329
GTTCAGG1455.5408236E-810.5735171
GTAGAAC6450.010.5169553
ATCCCGT1804.1836756E-1110.46788310
TCCCGTT1901.0913936E-1110.41278811
TTAGGAC7700.010.400293
ACTATCC1002.6288752E-510.3632058
GATATAC4750.010.3286561
Produced by FastQC (version 0.11.5)