##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512184_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1371022 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.918819683418647 31.0 28.0 34.0 22.0 34.0 2 25.96985168728146 31.0 25.0 34.0 2.0 34.0 3 27.650971319205674 31.0 26.0 34.0 16.0 34.0 4 32.17092358838881 35.0 32.0 37.0 22.0 37.0 5 32.602159556885304 35.0 33.0 37.0 25.0 37.0 6 32.809305029386834 35.0 33.0 37.0 26.0 37.0 7 32.79871001340606 36.0 33.0 37.0 25.0 37.0 8 32.80013303944065 36.0 33.0 37.0 25.0 37.0 9 34.25203607236062 39.0 35.0 39.0 26.0 39.0 10 34.11669324051693 38.0 34.0 39.0 25.0 39.0 11 34.12023804140269 38.0 35.0 39.0 25.0 39.0 12 34.004215103769305 38.0 34.0 39.0 25.0 39.0 13 33.99429695511815 38.0 34.0 39.0 25.0 39.0 14 34.99732024723163 39.0 34.0 41.0 25.0 41.0 15 34.92110775757063 39.0 34.0 41.0 24.0 41.0 16 34.864283724112376 39.0 34.0 41.0 24.0 41.0 17 34.8468886713707 39.0 34.0 41.0 24.0 41.0 18 34.79394641369723 39.0 34.0 41.0 24.0 41.0 19 34.82050032749292 39.0 34.0 41.0 23.0 41.0 20 34.72871332480442 39.0 34.0 41.0 22.0 41.0 21 34.64415669478681 39.0 34.0 41.0 21.0 41.0 22 34.53299144725614 39.0 34.0 41.0 19.0 41.0 23 34.40556971368804 39.0 34.0 41.0 18.0 41.0 24 34.27582416620594 39.0 34.0 41.0 18.0 41.0 25 34.140019634987624 39.0 34.0 41.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7038424581115947 1101 2 3.618412610638252 1101 3 2.1784995699788894 1101 4 1.1576747550888058 1101 5 0.8147077401495721 1101 6 0.6798939477031425 1101 7 0.49925706173404194 1101 8 0.4015241398822482 1101 9 0.4856697409752826 1101 10 0.4617822069747888 1101 11 0.5430750726968654 1101 12 0.5487868508296785 1101 13 0.5038610410661732 1101 14 0.588300989407017 1101 15 0.6549461407582129 1101 16 0.7499987522381275 1101 17 0.5836676946150234 1101 18 0.9324341615692475 1101 19 0.7710679983953526 1101 20 0.8815712470833432 1101 21 0.7685964563106538 1101 22 0.9251680784361156 1101 23 0.9060123308379886 1101 24 0.892130742720532 1101 25 0.8588145660717998 1102 1 1.4925401879138853 1102 2 4.451037843378543 1102 3 2.839995741830304 1102 4 2.028195354791059 1102 5 1.3483292373839006 1102 6 1.395613910438783 1102 7 1.2713860150797345 1102 8 1.126204522839629 1102 9 1.354347206014026 1102 10 1.1671832069968175 1102 11 1.2465882363711174 1102 12 1.3857701738621913 1102 13 1.233548012663114 1102 14 1.4297842819905568 1102 15 1.290153835148999 1102 16 1.3971016296013303 1102 17 1.4413725157888493 1102 18 1.569992905413045 1102 19 1.3743787735743922 1102 20 1.4307592960556548 1102 21 1.5803971826806276 1102 22 1.4700420909499954 1102 23 1.4080031754147626 1102 24 1.219716346445935 1102 25 1.1112268373967709 1103 1 0.9909763848737612 1103 2 3.7233527055792415 1103 3 2.4272130769959226 1103 4 1.3897275348093245 1103 5 1.0332873604237278 1103 6 0.7546944388604686 1103 7 0.637791758772174 1103 8 0.5891459843139444 1103 9 0.7680369261775439 1103 10 0.9213173099228129 1103 11 0.9119709406103595 1103 12 0.7495210459116279 1103 13 0.8142220340780497 1103 14 0.9134163942275038 1103 15 0.9924643479450452 1103 16 0.9208207547142422 1103 17 0.9148368103984339 1103 18 0.8371368087496975 1103 19 1.0444956374925454 1103 20 1.0887771323321687 1103 21 1.1663722733551865 1103 22 1.1692101279063039 1103 23 1.2249787406824737 1103 24 1.27680754887664 1103 25 1.2760810456006695 1104 1 1.2309054477966477 1104 2 4.105377675812925 1104 3 2.424578339585043 1104 4 1.745094317990997 1104 5 1.4024966762048976 1104 6 1.140559144377157 1104 7 1.040298106528816 1104 8 1.0447284238608248 1104 9 1.1236153012474759 1104 10 1.0241306452325958 1104 11 0.9544273909352796 1104 12 0.9802440685435911 1104 13 1.0749044358492057 1104 14 1.2027904169598855 1104 15 0.8969318058298938 1104 16 1.0490603819299835 1104 17 0.9845294403062894 1104 18 0.7441738528707873 1104 19 0.9936412605290457 1104 20 0.9379647151160029 1104 21 1.1262277931539657 1104 22 1.214198508283964 1104 23 1.123379369002798 1104 24 1.0592074073372117 1104 25 0.949581612236571 1105 1 0.9454933630646174 1105 2 3.71831204321656 1105 3 2.286178061775111 1105 4 1.675611738988792 1105 5 1.2384933193348857 1105 6 0.8024945535680388 1105 7 0.46098915873393764 1105 8 0.6209640992862191 1105 9 0.8090545882019882 1105 10 0.9802887653853958 1105 11 0.8385569198159359 1105 12 0.72667247460911 1105 13 0.8322293577151569 1105 14 0.8233691876509326 1105 15 0.7440501065591434 1105 16 0.7060652436037884 1105 17 0.7388053981717846 1105 18 0.8873651944612533 1105 19 0.8062856638164604 1105 20 0.9889138990493294 1105 21 1.0201860059100767 1105 22 1.161911785283614 1105 23 0.7012269208027746 1105 24 0.6235991193409163 1105 25 0.7911674439300569 1106 1 0.5102873073191176 1106 2 3.266952455187351 1106 3 1.6037597031860535 1106 4 0.9560462218444528 1106 5 0.5074196345319351 1106 6 0.327071819196334 1106 7 0.23064570904136872 1106 8 -0.042201941263634524 1106 9 -0.028131403665526022 1106 10 0.22382709921117794 1106 11 0.12560207057642003 1106 12 0.2966267469769548 1106 13 0.12301545088696741 1106 14 0.10726377490629346 1106 15 0.10370623476535457 1106 16 0.3107578835655147 1106 17 0.31059665504852063 1106 18 0.16503800973568872 1106 19 -0.04822899013088744 1106 20 0.12193487747995846 1106 21 -0.1518056083456898 1106 22 -0.053844409051784226 1106 23 0.02661349516996836 1106 24 -0.020146888285047737 1106 25 -0.3465461744623042 1107 1 0.5061584000036099 1107 2 3.276487108383158 1107 3 1.8910072780201617 1107 4 1.0820411497206877 1107 5 0.6152548433553591 1107 6 0.45286070205333573 1107 7 0.3965599911105926 1107 8 0.3705163743770541 1107 9 0.36059441073274456 1107 10 0.3755157342269797 1107 11 0.4882877446292113 1107 12 0.3042594872102313 1107 13 0.4183057208112828 1107 14 0.35211785393043016 1107 15 0.30192047567895486 1107 16 0.4587622628228658 1107 17 0.3107269011228553 1107 18 0.11615302822902862 1107 19 0.20065310888068666 1107 20 0.19522391647210924 1107 21 0.4372343980171465 1107 22 0.4450322185570741 1107 23 0.5810016402149927 1107 24 0.4505960534720046 1107 25 0.5674503142316425 1108 1 0.9228018549464103 1108 2 3.515218440416632 1108 3 2.0876769696985846 1108 4 1.2315665822823902 1108 5 0.8738529151349965 1108 6 0.8200561966826925 1108 7 0.8624417520867453 1108 8 0.749437034208988 1108 9 0.8590180102171985 1108 10 0.6034039465648391 1108 11 0.7008347546097582 1108 12 0.9041762245104223 1108 13 0.8916746354710057 1108 14 0.8886406278761925 1108 15 0.9220738427766122 1108 16 0.7947297524325023 1108 17 0.7453062797275365 1108 18 0.8536813440362607 1108 19 0.9092950583060784 1108 20 1.0028845840312997 1108 21 0.7333640801712775 1108 22 0.9436470027778938 1108 23 0.9508098265157798 1108 24 0.9192881801551067 1108 25 0.9447386657400045 1109 1 0.9083188737460439 1109 2 3.4150795467250568 1109 3 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2315 20 -1.8084960379731143 2315 21 -1.746985072564776 2315 22 -1.6543224861661372 2315 23 -1.8905999352611929 2315 24 -1.78368752358773 2315 25 -1.7512490189359937 2316 1 -1.5808753157165114 2316 2 -0.043997130818578256 2316 3 -0.5047978253422514 2316 4 -1.3256238431783487 2316 5 -1.2727810198236007 2316 6 -1.2702725099516528 2316 7 -1.26902546216699 2316 8 -1.3590099080010205 2316 9 -1.5533989382200701 2316 10 -1.5261281855765674 2316 11 -2.1069307916649507 2316 12 -1.4398438056480316 2316 13 -1.4663104088145786 2316 14 -1.5554555704079647 2316 15 -1.9718954944063043 2316 16 -1.5510941242163057 2316 17 -1.688262763075862 2316 18 -2.183970413234448 2316 19 -1.982849846406566 2316 20 -1.9572069206494334 2316 21 -1.9755038453280633 2316 22 -1.8706468723784297 2316 23 -1.699743164369778 2316 24 -1.7405722469010527 2316 25 -1.7401157141845474 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61913.0 3 2.0 4 553.0 5 2654.0 6 3043.0 7 2754.0 8 2628.0 9 2560.0 10 2467.0 11 2493.0 12 2313.0 13 2346.0 14 2373.0 15 2526.0 16 2628.0 17 2881.0 18 3169.0 19 4116.0 20 5241.0 21 6841.0 22 8579.0 23 10253.0 24 11849.0 25 13592.0 26 14918.0 27 16750.0 28 19509.0 29 23278.0 30 29110.0 31 36789.0 32 46799.0 33 60253.0 34 80162.0 35 109601.0 36 154717.0 37 191888.0 38 427474.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.24301287652569 19.68743025276035 13.679795072580891 24.389761798133073 2 15.268766662156164 21.18702209434549 41.43285245035083 22.111358793147517 3 18.48650131070107 25.131763020578806 30.40658720283117 25.975148465888946 4 11.731978042657229 17.287833455626533 39.601698586893576 31.378489914822666 5 12.403375000547037 38.42009829163938 36.48446195611741 12.692064751696181 6 30.25034663998092 38.38378607444536 18.745254442133916 12.620612843439808 7 26.76126918315974 32.47666363465942 22.885940847410772 17.876126334770074 8 23.77883068251275 38.4624754380309 21.20396317491623 16.554730704540116 9 25.577634786312693 16.20747150665708 21.321612636412837 36.8932810706174 10 14.38967673794022 30.009219803320587 34.979962974903756 20.621140483835436 11 33.04991221652722 23.405692395332707 25.39309271349765 18.15130267464243 12 22.43346895071823 25.303169770695266 32.88493042375935 19.378430854827158 13 28.233244980751586 22.75499590816194 26.740854632529604 22.270904478556872 14 20.162142646141724 23.212510439346033 28.61420188692319 28.011145027589052 15 22.180460999167046 31.963454999263323 24.37218367028392 21.483900331285714 16 20.907760779914543 29.263352448027824 29.07699511751088 20.751891654546753 17 20.46571165993202 29.491181747895727 29.0875406631559 20.955565929016355 18 20.157360039507786 28.54743961676863 31.417662810961517 19.877537532762066 19 22.277462794672104 27.763493518836626 28.814717722376738 21.14432596411454 20 22.11831757623145 28.41456956927022 29.68588396101594 19.781228893482382 21 21.821395329837863 27.754873383927244 28.586798899421993 21.836932386812904 22 21.658766009498294 28.90429713570482 29.008601857207566 20.42833499758932 23 21.354010528242956 28.45448398553151 29.139687770563956 21.051817715661585 24 21.851862658564876 27.61935676823086 29.95010540597714 20.578675167227132 25 21.7251951049252 28.132529109822606 29.563237775260426 20.579038009991766 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 355.0 1 355.0 2 465.0 3 575.0 4 575.0 5 575.0 6 1478.5 7 2382.0 8 2382.0 9 2382.0 10 3449.0 11 4516.0 12 4516.0 13 4516.0 14 7049.5 15 9583.0 16 9583.0 17 9583.0 18 16138.0 19 22693.0 20 22693.0 21 22693.0 22 34379.5 23 46066.0 24 46066.0 25 46066.0 26 63920.5 27 81775.0 28 81775.0 29 81775.0 30 103792.0 31 125809.0 32 125809.0 33 125809.0 34 136942.0 35 148075.0 36 148075.0 37 148075.0 38 155280.5 39 162486.0 40 162486.0 41 162486.0 42 167456.5 43 172427.0 44 172427.0 45 172427.0 46 171838.0 47 171249.0 48 171249.0 49 171249.0 50 160654.5 51 150060.0 52 150060.0 53 150060.0 54 130497.5 55 110935.0 56 110935.0 57 110935.0 58 93140.0 59 75345.0 60 75345.0 61 75345.0 62 60756.0 63 46167.0 64 46167.0 65 46167.0 66 34999.5 67 23832.0 68 23832.0 69 23832.0 70 17156.5 71 10481.0 72 10481.0 73 10481.0 74 7291.0 75 4101.0 76 4101.0 77 4101.0 78 2779.0 79 1457.0 80 1457.0 81 1457.0 82 971.0 83 485.0 84 485.0 85 485.0 86 302.5 87 120.0 88 120.0 89 120.0 90 79.0 91 38.0 92 38.0 93 38.0 94 23.0 95 8.0 96 8.0 97 8.0 98 5.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.826643190262446 3 0.0 4 0.0 5 0.0 6 2.1881486949151802E-4 7 0.0067832609542370585 8 0.0 9 0.0 10 0.00437629738983036 11 0.0025528401440677102 12 5.83506318644048E-4 13 0.0 14 5.10568028813542E-4 15 0.0 16 0.0 17 1.45876579661012E-4 18 0.011159558344067419 19 0.003573976201694794 20 0.0 21 0.007731458722033636 22 0.002844593303389734 23 0.003136346462711758 24 0.009627854257626791 25 0.0067832609542370585 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1371022.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.82693314508704 #Duplication Level Percentage of deduplicated Percentage of total 1 90.33478216500782 73.01483398727089 2 7.269898949883355 11.752072727875209 3 1.3001148921891232 3.1525289841570694 4 0.41363288494748046 1.3373071015303792 5 0.20210504714470054 0.8167765566924688 6 0.11562403392603349 0.5607321636062871 7 0.07124188252714317 0.4030784013306082 8 0.04376855151203565 0.28301422295364836 9 0.03605548605307771 0.2622828924653157 >10 0.1811171208155277 2.6384719583616096 >50 0.015231141381940358 0.8424882333024866 >100 0.013689947831112038 2.171690575057167 >500 0.0016427380684694733 0.9241312610440979 >1k 0.0010951587123129822 1.840590934352836 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4637 0.3382148499440563 No Hit TATCAACGCAGAGTACTTTTTTTTT 2936 0.21414681894236562 No Hit GGTATCAACGCAGAGTACTTTTTTT 2828 0.206269483640671 No Hit GTCCTACAGTGGACATTTCTAAATT 2464 0.1797199461423668 No Hit CTGTAGGACGTGGAATATGGCAAGA 2248 0.1639652755389775 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2115 0.1542644829915202 No Hit CTTTAGGACGTGAAATATGGCGAGG 1952 0.1423755417491477 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1412 0.10298886524067448 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 7.2938289830506E-5 7 0.0 0.0 0.0 7.2938289830506E-5 7.2938289830506E-5 8 0.0 0.0 0.0 7.2938289830506E-5 7.2938289830506E-5 9 0.0 0.0 0.0 1.45876579661012E-4 1.45876579661012E-4 10 0.0 0.0 0.0 1.45876579661012E-4 1.45876579661012E-4 11 0.0 0.0 0.0 1.45876579661012E-4 1.45876579661012E-4 12 0.0 0.0 0.0 1.45876579661012E-4 1.45876579661012E-4 13 0.0 0.0 0.0 2.1881486949151802E-4 1.45876579661012E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCAACG 65 2.7396454E-5 14.152245 2 TAGGACC 425 0.0 13.300855 4 TCCAACG 205 0.0 12.870653 18 CCAACGA 145 1.1277734E-10 11.697712 19 TGGCGAA 65 8.58778E-4 11.597732 18 GGTATCA 1215 0.0 11.188492 1 CCTAGAC 85 5.7910707E-5 11.084044 3 GAACAGT 485 0.0 11.0726185 6 AGGACCT 910 0.0 10.974423 5 CGTTTCC 175 2.3646862E-11 10.768145 14 AGAACAG 585 0.0 10.629315 5 GTCATAT 135 2.3582106E-7 10.599624 1 CAGCTTA 80 4.0719833E-4 10.598732 9 GTTCAGG 145 5.5408236E-8 10.573517 1 GTAGAAC 645 0.0 10.516955 3 ATCCCGT 180 4.1836756E-11 10.467883 10 TCCCGTT 190 1.0913936E-11 10.412788 11 TTAGGAC 770 0.0 10.40029 3 ACTATCC 100 2.6288752E-5 10.363205 8 GATATAC 475 0.0 10.328656 1 >>END_MODULE