FastQCFastQC Report
Thu 26 May 2016
SRR1512184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512184_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371022
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60940.4444859382271036No Hit
TATCAACGCAGAGTACTTTTTTTTT41550.30305859424575243No Hit
GGTATCAACGCAGAGTACTTTTTTT39220.28606397271524453No Hit
GTCCTACAGTGGACATTTCTAAATT27170.19817333346948482No Hit
CTGTAGGACGTGGAATATGGCAAGA26310.1919006405440613No Hit
GTCCTAAAGTGTGTATTTCTCATTT23220.16936270898643493No Hit
CTTTAGGACGTGAAATATGGCGAGG21840.15929722498982513No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19510.1423026034593172No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15040.10969918790508103No Hit
TTGTAGAACAGTGTATATCAATGAG14270.10408293958813206No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCG350.002174369516.2808389
GCGCCAC601.4710895E-615.82859213
TGGACCG551.1274169E-515.546475
GAAGCGA500.001502777913.2960176
CGGTTTC1001.44757E-712.34630113
TCCAACG1650.012.08728918
GACTGAC802.8745804E-511.8714447
TAGGACC4250.011.8478324
GCCTTAG1001.8403443E-611.4445851
AGGCGTG752.0792501E-411.3965867
TGCGCCA752.0792501E-411.39658612
GCCGGTT1001.935803E-611.39658511
CCAACGT855.335843E-511.17353219
CTAGACA1451.2150849E-911.1386594
GGTATCA13200.011.126681
TGTAGAA7350.011.1163722
AGTCGTC1201.2797864E-711.0800158
CCCTTAC1303.081368E-811.0044091
CTACACT2600.010.9622554
CCTACAC1855.456968E-1210.7844883
Produced by FastQC (version 0.11.5)