FastQCFastQC Report
Thu 26 May 2016
SRR1512183_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512183_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485621
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62560.42110336350926647No Hit
GTCCTACAGTGGACATTTCTAAATT45820.30842321157280356No Hit
CTGTAGGACGTGGAATATGGCAAGA42280.28459479234609636No Hit
GTCCTAAAGTGTGTATTTCTCATTT38860.26157411614402326No Hit
GGTATCAACGCAGAGTACTTTTTTT38510.25921819898884035No Hit
TATCAACGCAGAGTACTTTTTTTTT37940.2553814196218282No Hit
CTTTAGGACGTGAAATATGGCGAGG30020.20207037999597474No Hit
GTCCTACAGTGTGCATTTCTCATTT20210.13603738773213356No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20100.13529695662621893No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19100.12856576475426773No Hit
CTGAAGGACCTGGAATATGGCGAGA15990.10763175803249954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG350.001378227617.4291211
GCCGGTT753.6379788E-916.33922411
GGTATCA10600.014.5790771
CCGGTTT851.8995706E-814.417932512
GCATCCG400.005555640414.14066413
CGTATAA450.007348099313.5559841
CGGTAGG1005.9462764E-813.2170851
CGGTTTC958.167808E-812.90025513
GTACTAG801.4287483E-512.70873451
TCCAACG905.92845E-712.57032618
AAGGCGT604.3651953E-412.5699036
TAGGACC6850.012.5240274
TGGCGAA550.00324884111.99894718
CTAGGAC1850.011.7205863
CCAACGT658.553551E-411.60337719
GTCCTAA11900.011.192231
GTCCTAC20950.011.1618481
CTTACAC1701.2732926E-1111.091093
GGCGAGG9800.010.96696819
GACGTGG12250.010.928127
Produced by FastQC (version 0.11.5)