##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512183_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1485621 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.02457625464368 31.0 30.0 34.0 25.0 34.0 2 27.05892014181275 31.0 27.0 34.0 10.0 34.0 3 28.76448434694986 31.0 28.0 34.0 16.0 34.0 4 33.41152285811792 35.0 33.0 37.0 28.0 37.0 5 33.843872023887656 35.0 35.0 37.0 30.0 37.0 6 34.07059068228034 37.0 35.0 37.0 30.0 37.0 7 34.09239503211115 37.0 35.0 37.0 30.0 37.0 8 34.102271709944866 37.0 35.0 37.0 30.0 37.0 9 35.680996027923676 39.0 35.0 39.0 30.0 39.0 10 35.55711248023554 39.0 35.0 39.0 30.0 39.0 11 35.57981544418126 39.0 35.0 39.0 30.0 39.0 12 35.47067926476537 39.0 35.0 39.0 30.0 39.0 13 35.48241779027087 39.0 35.0 39.0 30.0 39.0 14 36.59410845700216 40.0 36.0 41.0 30.0 41.0 15 36.53777847782173 40.0 36.0 41.0 30.0 41.0 16 36.49775884966623 40.0 36.0 41.0 30.0 41.0 17 36.49182193843517 40.0 36.0 41.0 30.0 41.0 18 36.45719197561154 40.0 36.0 41.0 30.0 41.0 19 36.478656400252824 40.0 37.0 41.0 30.0 41.0 20 36.416432589469316 40.0 36.0 41.0 30.0 41.0 21 36.34280210093961 40.0 36.0 41.0 30.0 41.0 22 36.25777772392824 40.0 36.0 41.0 30.0 41.0 23 36.143995002763155 40.0 36.0 41.0 29.0 41.0 24 36.04849285248391 40.0 36.0 41.0 29.0 41.0 25 35.928328961424214 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37436184095550473 1101 2 3.4819137469050503 1101 3 1.9108291272597668 1101 4 0.7356701846266063 1101 5 0.5127017874405482 1101 6 0.25366026150164345 1101 7 0.17795010174815218 1101 8 0.11290788154325071 1101 9 0.033733561320090644 1101 10 -0.007569679131108842 1101 11 0.11999636027724137 1101 12 0.005991256489579655 1101 13 -0.12702810164400802 1101 14 -0.08514018630459219 1101 15 0.0251018190540222 1101 16 0.35384718083301436 1101 17 -0.011725001752864728 1101 18 -0.07526239426447034 1101 19 -0.19290511258862608 1101 20 -0.2023158103536602 1101 21 -0.055889120913356294 1101 22 -0.159677375935523 1101 23 -0.07432048869718955 1101 24 -0.15911906032960133 1101 25 0.10365264774956273 1102 1 1.1595065568431657 1102 2 4.183050440841459 1102 3 2.5298226583387127 1102 4 1.637304393808769 1102 5 0.9746708905115256 1102 6 1.049119317850021 1102 7 0.8886650694411742 1102 8 0.8246354700758474 1102 9 0.8505771191608247 1102 10 0.7797841775291872 1102 11 0.9589440247587504 1102 12 0.9436790945981741 1102 13 1.054532980604506 1102 14 1.2118507353826118 1102 15 1.1916062218146735 1102 16 1.0395241223324092 1102 17 1.0181513108210325 1102 18 1.076570529411292 1102 19 1.1156056057654453 1102 20 1.0775804912521423 1102 21 1.0767190573756338 1102 22 1.064807447921126 1102 23 1.09392366533406 1102 24 1.0979196657153096 1102 25 1.0452357602628837 1103 1 0.7719690889617041 1103 2 3.698278538650836 1103 3 2.241409426490069 1103 4 1.1734637089489368 1103 5 0.7308140946874246 1103 6 0.6200754388476781 1103 7 0.5636029900838864 1103 8 0.5465257932481222 1103 9 0.582788079291582 1103 10 0.5811634990346235 1103 11 0.5235897184022917 1103 12 0.5874937250079739 1103 13 0.6459941695433216 1103 14 0.8982964876441741 1103 15 0.8305156389969426 1103 16 0.693316402375487 1103 17 0.6866974401438881 1103 18 0.5668212383117037 1103 19 0.5649243960765702 1103 20 0.587975749306274 1103 21 0.737959511719211 1103 22 0.6939383549000908 1103 23 0.8806314005748135 1103 24 0.9275704929306059 1103 25 0.8481920143198423 1104 1 1.0492566516146944 1104 2 3.9147436098891895 1104 3 2.2600901047114874 1104 4 1.3956982351174858 1104 5 1.092170985843829 1104 6 0.7470899734446803 1104 7 0.5950393238864606 1104 8 0.6923555026458459 1104 9 0.734078798130966 1104 10 0.6945880509368578 1104 11 0.5655028874106947 1104 12 0.5572481342541664 1104 13 0.7895432652779917 1104 14 0.927374648089625 1104 15 0.6598412731483165 1104 16 1.0026226690194449 1104 17 0.8709160598503303 1104 18 0.7232703492893151 1104 19 0.8129531245459063 1104 20 0.8635596886214216 1104 21 0.9116909848154151 1104 22 1.0853112959816187 1104 23 1.0484867181212394 1104 24 1.132271596699205 1104 25 1.00044841859863 1105 1 0.9568939513858581 1105 2 3.867846056641522 1105 3 2.3528070559524643 1105 4 1.419897319785683 1105 5 0.9133578367503574 1105 6 0.7998623726701695 1105 7 0.5295952999470188 1105 8 0.6665765904824994 1105 9 0.689592792146108 1105 10 0.5824687057511468 1105 11 0.8103189409224001 1105 12 0.7032655607635832 1105 13 0.806732140476015 1105 14 0.8112815306807164 1105 15 0.9457724472373883 1105 16 0.733790157137733 1105 17 0.9054236289402766 1105 18 0.9694439982947713 1105 19 0.9479249604902833 1105 20 0.8787528764404655 1105 21 0.7869506222032001 1105 22 0.8860153973722191 1105 23 0.66054885506896 1105 24 0.7455065025256431 1105 25 0.9335983921948241 1106 1 0.32560447770164913 1106 2 3.162039039097664 1106 3 1.5946140734963237 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-1.3176922572322525 2315 14 -1.3656507848952444 2315 15 -1.690769846631163 2315 16 -1.6159843170802688 2315 17 -1.5089953419148543 2315 18 -1.4599059358712765 2315 19 -1.5825712851949234 2315 20 -1.615534248572878 2315 21 -1.6053966252257226 2315 22 -1.4071058438647484 2315 23 -1.5404342060320673 2315 24 -1.5992192189789307 2315 25 -1.3415991096734317 2316 1 -1.0938531494169936 2316 2 0.31354186395642003 2316 3 0.16914514694265037 2316 4 -0.7601160611242221 2316 5 -0.8420413603950507 2316 6 -0.8985854417812149 2316 7 -0.8409064841743046 2316 8 -0.9278837128181294 2316 9 -0.6994839250706661 2316 10 -0.9805732210998741 2316 11 -1.461545465857256 2316 12 -0.9520109780410522 2316 13 -0.930118885393739 2316 14 -1.0931207886769982 2316 15 -1.3365602913888424 2316 16 -0.8623940533885843 2316 17 -0.8839050954129775 2316 18 -1.3866599267721327 2316 19 -1.6594033178326413 2316 20 -1.4052368648619407 2316 21 -1.2020145874310586 2316 22 -1.1688284920122598 2316 23 -1.0628811181692228 2316 24 -0.8752495188838125 2316 25 -0.8681009833627087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36484.0 3 0.0 4 335.0 5 1689.0 6 2072.0 7 1862.0 8 1747.0 9 1695.0 10 1756.0 11 1625.0 12 1670.0 13 1649.0 14 1658.0 15 1727.0 16 1852.0 17 1920.0 18 2342.0 19 2740.0 20 3716.0 21 4643.0 22 5880.0 23 7173.0 24 8434.0 25 9765.0 26 11397.0 27 13057.0 28 15479.0 29 19142.0 30 24630.0 31 32770.0 32 43506.0 33 60003.0 34 85211.0 35 123999.0 36 182845.0 37 236745.0 38 532403.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.22066058570793 19.59806707094205 12.604695275578361 25.576577067771662 2 15.394493188531094 20.995598877180147 41.47392245806666 22.135985476222107 3 18.630525551267787 24.814808083622943 30.08748530075975 26.46718106434952 4 11.742294972943974 16.68393217381822 39.17742142848007 32.39635142475773 5 12.284223230554765 38.163098125295754 36.69078452714387 12.861894117005615 6 30.88880796489544 38.07133592125572 18.23616956972144 12.803686544127396 7 27.22514075841252 31.998136684429195 22.921743438678877 17.854979118479406 8 23.894452218971058 38.87128682214374 20.85175155709296 16.382509401792248 9 25.85760432842562 15.544274077978166 21.273393415951983 37.32472817764423 10 14.616921980210817 29.357703059925587 35.24875148008258 20.776623479781012 11 33.54200618075511 22.57113219828767 25.881289382792836 18.00557223816438 12 23.27947022720511 24.698879523538352 32.86881284421702 19.15283740503952 13 28.690157180061405 22.694011460527282 26.48569184199739 22.13013951741393 14 19.691333842212465 23.232621037171064 28.443651059693675 28.632394060922795 15 21.646705317170394 33.15987051879315 23.89626964077648 21.29715452325997 16 20.474400940751377 28.702071389674757 29.976218699116398 20.847308970457473 17 20.175765118782138 29.728079675879215 29.408886127600688 20.687269077737966 18 20.056264603855585 27.507184541572734 32.527780564005866 19.908770290565815 19 22.42555319734705 27.13209759617553 29.011888198030277 21.430461008447143 20 22.478209449112526 27.9046270886047 29.92472508129597 19.692438380986808 21 21.24404509872161 27.36821131156932 28.667199357001927 22.72054423270714 22 21.882471728594506 29.3238422186322 28.95516962843296 19.838516424340334 23 20.445549886239718 28.90467022980923 29.91599240700602 20.733787476945032 24 22.140273071087073 27.060924661084613 30.740288958271904 20.05851330955641 25 21.119161399722522 28.160856479502506 30.27203213952258 20.447949981252396 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1047.0 1 1047.0 2 985.5 3 924.0 4 924.0 5 924.0 6 2235.0 7 3546.0 8 3546.0 9 3546.0 10 4564.5 11 5583.0 12 5583.0 13 5583.0 14 7839.0 15 10095.0 16 10095.0 17 10095.0 18 16676.0 19 23257.0 20 23257.0 21 23257.0 22 35389.5 23 47522.0 24 47522.0 25 47522.0 26 67078.5 27 86635.0 28 86635.0 29 86635.0 30 110983.0 31 135331.0 32 135331.0 33 135331.0 34 148559.5 35 161788.0 36 161788.0 37 161788.0 38 168300.0 39 174812.0 40 174812.0 41 174812.0 42 178931.5 43 183051.0 44 183051.0 45 183051.0 46 184440.5 47 185830.0 48 185830.0 49 185830.0 50 174031.5 51 162233.0 52 162233.0 53 162233.0 54 140903.0 55 119573.0 56 119573.0 57 119573.0 58 101521.0 59 83469.0 60 83469.0 61 83469.0 62 68071.5 63 52674.0 64 52674.0 65 52674.0 66 40202.5 67 27731.0 68 27731.0 69 27731.0 70 20218.0 71 12705.0 72 12705.0 73 12705.0 74 8856.5 75 5008.0 76 5008.0 77 5008.0 78 3496.0 79 1984.0 80 1984.0 81 1984.0 82 1282.5 83 581.0 84 581.0 85 581.0 86 380.5 87 180.0 88 180.0 89 180.0 90 115.5 91 51.0 92 51.0 93 51.0 94 29.0 95 7.0 96 7.0 97 7.0 98 5.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.270898836244237 3 0.0 4 0.0 5 0.0 6 1.3462383743902384E-4 7 0.006529256115792655 8 0.0 9 0.0 10 0.004173338960609739 11 0.0028271005862195 12 2.0193575615853573E-4 13 0.0 14 2.0193575615853573E-4 15 0.0 16 0.0 17 1.3462383743902384E-4 18 0.009423668620731667 19 0.0020193575615853575 20 0.0 21 0.005384953497560953 22 0.00141355029310975 23 0.002759788667499988 24 0.00989485205176825 25 0.006596568034512166 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1485621.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.97133187316524 #Duplication Level Percentage of deduplicated Percentage of total 1 87.7526593221649 65.78933744794833 2 8.727308040563871 13.085958149369151 3 1.8434978872365997 4.146284757344822 4 0.6255868369045786 1.8760431346022743 5 0.3095640923497907 1.1604216151785667 6 0.17174147498654563 0.7725412270561932 7 0.11140906462811868 0.584674017053952 8 0.07269401216552113 0.43599735290025565 9 0.0575288290939358 0.38817116444484584 >10 0.2864952076074798 3.8447943244887774 >50 0.021095347584026626 1.1101601611161436 >100 0.016336050453641428 2.4386048400386455 >500 0.0024503005566194115 1.222844951833243 >1k 0.0015427818319455552 2.7185218324959135 >5k 9.075187246738561E-5 0.4256450241289027 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6256 0.42110336350926647 No Hit GTCCTACAGTGGACATTTCTAAATT 4582 0.30842321157280356 No Hit CTGTAGGACGTGGAATATGGCAAGA 4228 0.28459479234609636 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3886 0.26157411614402326 No Hit GGTATCAACGCAGAGTACTTTTTTT 3851 0.25921819898884035 No Hit TATCAACGCAGAGTACTTTTTTTTT 3794 0.2553814196218282 No Hit CTTTAGGACGTGAAATATGGCGAGG 3002 0.20207037999597474 No Hit GTCCTACAGTGTGCATTTCTCATTT 2021 0.13603738773213356 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2010 0.13529695662621893 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1910 0.12856576475426773 No Hit CTGAAGGACCTGGAATATGGCGAGA 1599 0.10763175803249954 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.73119187195119E-5 0.0 10 6.73119187195119E-5 0.0 0.0 6.73119187195119E-5 0.0 11 6.73119187195119E-5 0.0 0.0 6.73119187195119E-5 0.0 12 6.73119187195119E-5 0.0 0.0 6.73119187195119E-5 0.0 13 6.73119187195119E-5 0.0 0.0 6.73119187195119E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTACG 35 0.0013782276 17.429121 1 GCCGGTT 75 3.6379788E-9 16.339224 11 GGTATCA 1060 0.0 14.579077 1 CCGGTTT 85 1.8995706E-8 14.4179325 12 GCATCCG 40 0.0055556404 14.140664 13 CGTATAA 45 0.0073480993 13.555984 1 CGGTAGG 100 5.9462764E-8 13.217085 1 CGGTTTC 95 8.167808E-8 12.900255 13 GTACTAG 80 1.4287483E-5 12.7087345 1 TCCAACG 90 5.92845E-7 12.570326 18 AAGGCGT 60 4.3651953E-4 12.569903 6 TAGGACC 685 0.0 12.524027 4 TGGCGAA 55 0.003248841 11.998947 18 CTAGGAC 185 0.0 11.720586 3 CCAACGT 65 8.553551E-4 11.603377 19 GTCCTAA 1190 0.0 11.19223 1 GTCCTAC 2095 0.0 11.161848 1 CTTACAC 170 1.2732926E-11 11.09109 3 GGCGAGG 980 0.0 10.966968 19 GACGTGG 1225 0.0 10.92812 7 >>END_MODULE