FastQCFastQC Report
Thu 26 May 2016
SRR1512183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512183_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485621
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79170.5329084605023758No Hit
TATCAACGCAGAGTACTTTTTTTTT56220.37842760704109596No Hit
GGTATCAACGCAGAGTACTTTTTTT53100.35742628840060825No Hit
CTGTAGGACGTGGAATATGGCAAGA43360.29186447956780365No Hit
GTCCTACAGTGGACATTTCTAAATT40270.27106509668347445No Hit
GTCCTAAAGTGTGTATTTCTCATTT35740.24057279750353558No Hit
CTTTAGGACGTGAAATATGGCGAGG35530.2391592472104258No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29310.1972912337668894No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23100.1554905322420725No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20640.13893180023707258No Hit
GTCCTACAGTGTGCATTTCTCATTT19500.13125824150304824No Hit
CTGAAGGACCTGGAATATGGCGAGA18990.1278253336483531No Hit
CTGTAGGACCTGGAATATGGCGAGA16340.10998767518768246No Hit
ATTTAGAAATGTCCACTGTAGGACG15610.10507390512115809No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAACG402.7667655E-416.6204647
TAGGACC6700.014.6039354
GGTATCA13650.012.50455951
CTGCGTT1001.4473699E-712.34663111
GGCGAGG9050.012.17634219
AGGACCT14700.011.9553385
GTTAGAC802.867251E-511.8745583
CTGTAGG23250.011.5247471
GGACCTG15050.011.3590276
GTCCTAT1604.1836756E-1111.3235251
TGTAGGA24350.011.2357222
GTAGGAC23350.011.1473373
TACGTCC600.00587707911.0829215
AATGTCC4600.010.9426668
GGACGTG23800.010.7344666
CCACCTT4350.010.6982313
TAAGACG803.771758E-410.6871024
TGGCGAG23350.010.61594718
AGGACGT24450.010.6069685
GACGTGG12000.010.2888597
Produced by FastQC (version 0.11.5)