##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512183_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1485621 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.659301396520377 31.0 30.0 34.0 26.0 34.0 2 29.863301609226042 31.0 30.0 34.0 26.0 34.0 3 29.92532011865745 31.0 30.0 34.0 26.0 34.0 4 33.55881412554077 35.0 35.0 37.0 28.0 37.0 5 33.04377697945842 35.0 33.0 37.0 26.0 37.0 6 32.956914313946825 35.0 33.0 37.0 26.0 37.0 7 32.72954138370419 35.0 33.0 37.0 26.0 37.0 8 32.58539762160066 35.0 33.0 37.0 25.0 37.0 9 33.89764145767999 37.0 33.0 39.0 25.0 39.0 10 33.621730575967895 37.0 33.0 39.0 25.0 39.0 11 33.62500732017116 37.0 33.0 39.0 25.0 39.0 12 33.37633151389217 37.0 33.0 39.0 23.0 39.0 13 33.34532091293809 37.0 33.0 39.0 22.0 39.0 14 34.09262591199236 38.0 33.0 40.0 21.0 41.0 15 33.99825662130517 38.0 33.0 40.0 19.0 41.0 16 33.87076919348878 38.0 33.0 40.0 18.0 41.0 17 33.78021716171217 38.0 33.0 40.0 18.0 41.0 18 33.694308979208024 38.0 33.0 40.0 17.0 41.0 19 33.716216316274476 38.0 33.0 40.0 18.0 41.0 20 33.55588807643403 38.0 32.0 40.0 18.0 41.0 21 33.39446669103358 38.0 32.0 40.0 17.0 41.0 22 33.48440012627716 38.0 33.0 40.0 16.0 41.0 23 33.30885131537586 38.0 33.0 40.0 10.0 41.0 24 33.12445569899725 38.0 32.0 40.0 2.0 41.0 25 32.61165869356989 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6247910031990891 1101 2 0.5593859713443479 1101 3 0.6718518864354976 1101 4 0.7645260937063014 1101 5 0.6476975402296574 1101 6 0.8310927451431027 1101 7 0.574870392994228 1101 8 0.4015627561745987 1101 9 0.5026421471317946 1101 10 0.7375382001266502 1101 11 0.7409907474629023 1101 12 0.5457467288749385 1101 13 0.3392254900830949 1101 14 0.19814654079127791 1101 15 0.5323910547695831 1101 16 0.4896133938607363 1101 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-0.027259664489712065 2313 25 -0.09012544764602382 2314 1 -0.49578719771387725 2314 2 -0.5697435614363471 2314 3 -0.45947782356204314 2314 4 -0.6068289784626941 2314 5 -0.6829931633141513 2314 6 -0.701739240198485 2314 7 -0.6583307056794752 2314 8 -0.860944561537007 2314 9 -0.890950809471299 2314 10 -0.9110232836841874 2314 11 -0.7989803177306101 2314 12 -0.8921362903200105 2314 13 -0.9438686948343786 2314 14 -1.1239326974238892 2314 15 -0.9261858338190336 2314 16 -0.7754646293047998 2314 17 -0.879759661822817 2314 18 -0.955404641267009 2314 19 -0.9375353890000966 2314 20 -0.855811833438203 2314 21 -0.8748734411246915 2314 22 -0.7222023250638685 2314 23 -0.9874681019006957 2314 24 -0.8529168196360004 2314 25 -1.259417639336231 2315 1 -0.8279792909707133 2315 2 -0.6248278989501088 2315 3 -0.7233869267954596 2315 4 -1.0115910659301974 2315 5 -0.8086335921168839 2315 6 -0.9012128288251304 2315 7 -1.0545008796237347 2315 8 -0.929141436321185 2315 9 -1.3061925247163444 2315 10 -1.3044318015897645 2315 11 -1.2441338629074323 2315 12 -1.1719745812479871 2315 13 -1.0687711757206273 2315 14 -1.610847444189666 2315 15 -1.1838025562048173 2315 16 -1.6034686230055968 2315 17 -1.2414347140674877 2315 18 -1.4711688458051952 2315 19 -1.5323371832665202 2315 20 -1.5883276688370245 2315 21 -1.453924246557964 2315 22 -1.1854775860314106 2315 23 -1.4616228723012696 2315 24 -1.1461528512752537 2315 25 -1.3367657503856698 2316 1 -0.9795929557235823 2316 2 -1.035652895225649 2316 3 -1.0353352198473367 2316 4 -1.1144759744231152 2316 5 -1.318388465427983 2316 6 -1.1917907502333236 2316 7 -1.3258032942190319 2316 8 -1.4371512001331972 2316 9 -1.2122802385862244 2316 10 -1.5326198810296034 2316 11 -1.275561419318663 2316 12 -1.6682856480475863 2316 13 -1.3615218890977374 2316 14 -1.6386376368301327 2316 15 -1.9300108279413308 2316 16 -1.9388835277167509 2316 17 -1.7816499304871911 2316 18 -1.6080863690973857 2316 19 -1.6639666626444836 2316 20 -1.6045676844224488 2316 21 -1.6397217964370618 2316 22 -1.5221860812354535 2316 23 -1.4626052126365856 2316 24 -1.440391972990568 2316 25 -1.315632827315799 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43054.0 3 1.0 4 779.0 5 6517.0 6 7625.0 7 6285.0 8 5054.0 9 4599.0 10 4217.0 11 4020.0 12 3824.0 13 3634.0 14 3519.0 15 3658.0 16 3690.0 17 3777.0 18 4168.0 19 4599.0 20 5697.0 21 7281.0 22 9332.0 23 11548.0 24 13906.0 25 16301.0 26 18709.0 27 21388.0 28 24975.0 29 30146.0 30 37286.0 31 47728.0 32 60162.0 33 75958.0 34 96245.0 35 126500.0 36 178613.0 37 275883.0 38 314943.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.67874945015294 21.622452544229326 13.890741420189912 23.80805658542782 2 15.363743511972434 22.464410505775025 40.39960393667025 21.772242045582285 3 19.31441464545803 26.244378613387937 30.821050590964987 23.620156150189047 4 13.338058630027444 18.3690860589612 38.274499350776544 30.01835596023481 5 12.408614632162532 39.008102645176315 36.23055304559539 12.352729677065758 6 30.989061140088893 38.884951141643796 17.703640430500105 12.422347287767204 7 27.3136957541661 32.531244509871634 22.78804621097844 17.36701352498383 8 24.246560865792823 39.361788773852815 20.47776653668735 15.913883823667005 9 26.040490811586537 15.454951161837375 21.015117583825216 37.489440442750876 10 14.713173817548352 30.29541181768432 34.77017355032003 20.22124081444729 11 33.95650707683858 22.724301823951063 25.545546273241964 17.7736448259684 12 23.359726336663254 25.32395543681733 32.56819875324864 18.748119473270776 13 29.16335996865957 22.802114401990817 26.34204820744995 21.692477421899664 14 19.972388650941255 23.526457959331484 28.387724729254636 28.11342866047262 15 21.97330274679747 33.432618413444615 23.71472939598996 20.879349443767957 16 20.680173476276924 29.334264930288413 29.44472378890713 20.540837804527534 17 20.40715633395058 30.211271919284933 29.078816198747866 20.30275554801662 18 20.306188455871315 27.952620486651707 32.21508042764608 19.526110629830892 19 22.740389372525023 27.38046917753586 29.188130754748354 20.691010695190766 20 22.83321250843923 28.16344141608122 29.75213732169914 19.251208753780404 21 21.610424192980577 27.45081013259775 28.909526723168295 22.029238951253383 22 22.303333084279235 29.22185402602683 29.01870665533134 19.4561062343626 23 20.732878708634303 29.107625699959815 29.913551302788534 20.245944288617352 24 22.34102775876216 27.397633716809334 30.53712891780609 19.724209606622416 25 21.427917496903444 28.469088265388553 30.16499272981851 19.938001507889496 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1152.0 1 1152.0 2 1113.0 3 1074.0 4 1074.0 5 1074.0 6 2401.5 7 3729.0 8 3729.0 9 3729.0 10 4955.0 11 6181.0 12 6181.0 13 6181.0 14 8388.0 15 10595.0 16 10595.0 17 10595.0 18 17519.0 19 24443.0 20 24443.0 21 24443.0 22 37129.0 23 49815.0 24 49815.0 25 49815.0 26 70413.0 27 91011.0 28 91011.0 29 91011.0 30 114465.5 31 137920.0 32 137920.0 33 137920.0 34 151159.5 35 164399.0 36 164399.0 37 164399.0 38 169631.5 39 174864.0 40 174864.0 41 174864.0 42 179106.5 43 183349.0 44 183349.0 45 183349.0 46 183309.5 47 183270.0 48 183270.0 49 183270.0 50 171341.5 51 159413.0 52 159413.0 53 159413.0 54 137969.0 55 116525.0 56 116525.0 57 116525.0 58 98984.0 59 81443.0 60 81443.0 61 81443.0 62 65800.0 63 50157.0 64 50157.0 65 50157.0 66 38344.0 67 26531.0 68 26531.0 69 26531.0 70 19390.0 71 12249.0 72 12249.0 73 12249.0 74 8555.0 75 4861.0 76 4861.0 77 4861.0 78 3364.5 79 1868.0 80 1868.0 81 1868.0 82 1230.5 83 593.0 84 593.0 85 593.0 86 366.0 87 139.0 88 139.0 89 139.0 90 85.5 91 32.0 92 32.0 93 32.0 94 19.0 95 6.0 96 6.0 97 6.0 98 4.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38125470762731545 2 0.0 3 0.0 4 0.0 5 0.028742189293231586 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0067985037906707025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1485621.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.16931768347294 #Duplication Level Percentage of deduplicated Percentage of total 1 87.99802917444299 65.26753781358782 2 8.529442638736477 12.652458814708107 3 1.811033702599487 4.0296940207073275 4 0.6271965033126058 1.860749468166242 5 0.27876362947766575 1.0337854096663466 6 0.1796783859772471 0.7995973974240065 7 0.10709962357543024 0.5560454203322518 8 0.07865864378356979 0.46672463514677776 9 0.058613036145765574 0.3912560008459352 >10 0.28718605252973184 3.859339561982631 >50 0.022711175615345938 1.2067147212598726 >100 0.017071357911340353 2.655499522277201 >500 0.002709645535399619 1.469645344263319 >1k 0.0015354658033931174 2.4882412718557965 >5k 2.709645535399619E-4 1.262710597776429 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7917 0.5329084605023758 No Hit TATCAACGCAGAGTACTTTTTTTTT 5622 0.37842760704109596 No Hit GGTATCAACGCAGAGTACTTTTTTT 5310 0.35742628840060825 No Hit CTGTAGGACGTGGAATATGGCAAGA 4336 0.29186447956780365 No Hit GTCCTACAGTGGACATTTCTAAATT 4027 0.27106509668347445 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3574 0.24057279750353558 No Hit CTTTAGGACGTGAAATATGGCGAGG 3553 0.2391592472104258 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2931 0.1972912337668894 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2310 0.1554905322420725 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2064 0.13893180023707258 No Hit GTCCTACAGTGTGCATTTCTCATTT 1950 0.13125824150304824 No Hit CTGAAGGACCTGGAATATGGCGAGA 1899 0.1278253336483531 No Hit CTGTAGGACCTGGAATATGGCGAGA 1634 0.10998767518768246 No Hit ATTTAGAAATGTCCACTGTAGGACG 1561 0.10507390512115809 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 6.73119187195119E-5 0.0 0.0 0.0 6 0.0 6.73119187195119E-5 0.0 0.0 0.0 7 0.0 6.73119187195119E-5 0.0 0.0 0.0 8 0.0 6.73119187195119E-5 0.0 0.0 0.0 9 0.0 6.73119187195119E-5 0.0 0.0 0.0 10 0.0 6.73119187195119E-5 0.0 0.0 0.0 11 0.0 6.73119187195119E-5 0.0 0.0 0.0 12 0.0 6.73119187195119E-5 0.0 0.0 0.0 13 0.0 6.73119187195119E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACAACG 40 2.7667655E-4 16.620464 7 TAGGACC 670 0.0 14.603935 4 GGTATCA 1365 0.0 12.5045595 1 CTGCGTT 100 1.4473699E-7 12.346631 11 GGCGAGG 905 0.0 12.176342 19 AGGACCT 1470 0.0 11.955338 5 GTTAGAC 80 2.867251E-5 11.874558 3 CTGTAGG 2325 0.0 11.524747 1 GGACCTG 1505 0.0 11.359027 6 GTCCTAT 160 4.1836756E-11 11.323525 1 TGTAGGA 2435 0.0 11.235722 2 GTAGGAC 2335 0.0 11.147337 3 TACGTCC 60 0.005877079 11.082921 5 AATGTCC 460 0.0 10.942666 8 GGACGTG 2380 0.0 10.734466 6 CCACCTT 435 0.0 10.69823 13 TAAGACG 80 3.771758E-4 10.687102 4 TGGCGAG 2335 0.0 10.615947 18 AGGACGT 2445 0.0 10.606968 5 GACGTGG 1200 0.0 10.288859 7 >>END_MODULE