FastQCFastQC Report
Thu 26 May 2016
SRR1512182_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512182_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015445
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68360.33918067722016726No Hit
TATCAACGCAGAGTACTTTTTTTTT41770.20724951561565808No Hit
GGTATCAACGCAGAGTACTTTTTTT41120.20402442140569454No Hit
GTCCTACAGTGGACATTTCTAAATT36690.1820441639439429No Hit
CTGTAGGACGTGGAATATGGCAAGA33240.16492635621413634No Hit
GTCCTAAAGTGTGTATTTCTCATTT26370.13083959125652153No Hit
CTTTAGGACGTGAAATATGGCGAGG24950.12379400082860113No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20950.1039472672288254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAC652.0960579E-715.9490143
TACGACA400.005567081714.1366274
ATACCGT655.87836E-513.0491936
ATGCGCC604.3781663E-412.5658911
TGTAGGA20750.011.6423552
GTCCTAC20350.011.470481
TAGGACC7900.011.4524574
GACGTGG10200.011.3647397
CTGTAGG20500.011.2871051
GTCCTAG2450.011.2333231
GGTATCA14200.011.1981631
AGGACGT18800.011.1288335
GTAGGAC21050.011.058583
GTCCTAA9500.011.0515761
GGACGTG18350.011.0422336
ACTGCGC600.00623540210.9954268
TATGCCG600.00623655310.9951535
TAGGACG20150.010.8509434
AATCCCG3600.010.73389719
ACGTGGA10550.010.7199838
Produced by FastQC (version 0.11.5)