##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512182_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2015445 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.092732374239933 31.0 30.0 34.0 23.0 34.0 2 26.18050703442664 31.0 26.0 34.0 2.0 34.0 3 27.85236709510803 31.0 27.0 34.0 16.0 34.0 4 32.374837318805525 35.0 32.0 37.0 25.0 37.0 5 32.789872211844035 35.0 33.0 37.0 26.0 37.0 6 33.004099838993376 35.0 35.0 37.0 26.0 37.0 7 32.99832344717916 36.0 35.0 37.0 26.0 37.0 8 33.002690720907786 37.0 35.0 37.0 26.0 37.0 9 34.47927182334423 39.0 35.0 39.0 27.0 39.0 10 34.341101841032625 38.0 35.0 39.0 27.0 39.0 11 34.35288087742409 38.0 35.0 39.0 27.0 39.0 12 34.22388405538231 38.0 35.0 39.0 26.0 39.0 13 34.22403836373605 38.0 35.0 39.0 26.0 39.0 14 35.23423710396463 39.0 35.0 41.0 26.0 41.0 15 35.16161244787131 39.0 35.0 41.0 25.0 41.0 16 35.10564664379331 39.0 35.0 41.0 25.0 41.0 17 35.08629359769183 39.0 35.0 41.0 25.0 41.0 18 35.03645448027607 39.0 35.0 41.0 25.0 41.0 19 35.062733540235534 39.0 35.0 41.0 25.0 41.0 20 34.96203369479197 39.0 35.0 41.0 25.0 41.0 21 34.878722564991854 39.0 34.0 41.0 24.0 41.0 22 34.770385200290754 39.0 34.0 41.0 23.0 41.0 23 34.64393173715978 39.0 34.0 41.0 22.0 41.0 24 34.51552138609588 39.0 34.0 41.0 19.0 41.0 25 34.3736981162969 39.0 34.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7258111151152526 1101 2 3.6526801323321756 1101 3 2.2183660843586956 1101 4 1.0478629764405767 1101 5 0.7527549366912893 1101 6 0.653648923167971 1101 7 0.5927480889447594 1101 8 0.5291807033181612 1101 9 0.4222648206084685 1101 10 0.3975269543469082 1101 11 0.5250766847224142 1101 12 0.4410457142412483 1101 13 0.5280192524949143 1101 14 0.5192157931252979 1101 15 0.5823144482136939 1101 16 0.653664815396553 1101 17 0.5666738891183059 1101 18 0.5972187670494833 1101 19 0.3152753316087882 1101 20 0.3829513880102624 1101 21 0.5140442262981608 1101 22 0.4997907705949345 1101 23 0.4916203001928352 1101 24 0.5034486650191496 1101 25 0.5024036358130815 1102 1 1.2179965617651902 1102 2 3.9908077198148284 1102 3 2.2983525355615413 1102 4 1.4980895616582117 1102 5 1.0554581114512374 1102 6 1.1088488879009262 1102 7 1.061469228695465 1102 8 0.7815954081156491 1102 9 0.9492231881904587 1102 10 1.0375990843930367 1102 11 0.9906190467554268 1102 12 1.0848917534780753 1102 13 0.9486714585549336 1102 14 1.3032092955061003 1102 15 1.1635528238981792 1102 16 1.145457134880317 1102 17 1.2812208777195764 1102 18 1.4019612366693224 1102 19 1.1750073355082549 1102 20 1.140329985932972 1102 21 1.1859557506438634 1102 22 1.2595035785050257 1102 23 1.107388298804473 1102 24 0.9735779756766618 1102 25 1.123054335211144 1103 1 1.2667782067495175 1103 2 4.181365166890245 1103 3 2.6395710138789354 1103 4 1.6104932857990235 1103 5 1.292616103305825 1103 6 1.0588744328007067 1103 7 0.9340854177824056 1103 8 1.0158172107546264 1103 9 0.9361687526255267 1103 10 1.2045978615616377 1103 11 1.1918431647862633 1103 12 1.2179104529880291 1103 13 1.07637773027011 1103 14 1.2371075115446573 1103 15 1.4169989729442207 1103 16 1.3919123444583903 1103 17 1.6208959401611764 1103 18 1.5305137334487355 1103 19 1.4845308386923861 1103 20 1.5277707944062655 1103 21 1.6086734492119064 1103 22 1.6031883592665253 1103 23 1.7475086412830407 1103 24 1.649011662671498 1103 25 1.5818265288505984 1104 1 1.167795904839437 1104 2 4.016147189523075 1104 3 2.509358523067071 1104 4 1.5249335623803901 1104 5 1.2818557151820542 1104 6 0.9379964575818249 1104 7 0.9267838762815117 1104 8 1.0106660619478731 1104 9 1.1077104851975506 1104 10 1.007980675690618 1104 11 0.9102586139150546 1104 12 0.777257921485095 1104 13 0.901771003605873 1104 14 1.1011288722524952 1104 15 0.7771750060699603 1104 16 0.97005521393735 1104 17 0.8909566187248572 1104 18 0.7819733045917658 1104 19 0.9842380819984697 1104 20 0.7351230944418603 1104 21 0.899678868369989 1104 22 1.0053307485402527 1104 23 1.1058349365208002 1104 24 1.026032789595213 1104 25 1.0486439266016347 1105 1 0.8872353461895202 1105 2 3.6784880691245974 1105 3 2.380264136166673 1105 4 1.4656805677999714 1105 5 1.0565641763565736 1105 6 0.6841135035924708 1105 7 0.4545158534660487 1105 8 0.5463852790420134 1105 9 0.6388502634188953 1105 10 0.6187679675827624 1105 11 0.6330809818418217 1105 12 0.45287567108761095 1105 13 0.8172420040465056 1105 14 0.7744211136545616 1105 15 0.7606309518781558 1105 16 0.41471106309050043 1105 17 0.5405160869596628 1105 18 0.7700350866590071 1105 19 0.7890273800919729 1105 20 0.8184865478697603 1105 21 0.7033310482864508 1105 22 0.8338655108520996 1105 23 0.5756276967330294 1105 24 0.636213449245588 1105 25 0.8597878877934164 1106 1 0.18426583817297626 1106 2 2.8129012692619533 1106 3 1.2768187989559152 1106 4 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-1.4516957391006216 2315 18 -1.4989330090538502 2315 19 -1.7772631713306808 2315 20 -1.6336229889045342 2315 21 -1.550588443238972 2315 22 -1.571011684484965 2315 23 -1.4642555381776177 2315 24 -1.5326142024148126 2315 25 -1.5166496265865206 2316 1 -1.5383423796641544 2316 2 -0.15173019414805822 2316 3 -0.49416621695684526 2316 4 -1.1308045355138958 2316 5 -1.2731253426825049 2316 6 -1.0968701637847573 2316 7 -1.4148441516250614 2316 8 -1.4057481351111853 2316 9 -1.5364479704444776 2316 10 -1.4270294522898013 2316 11 -1.9820487308760661 2316 12 -1.4024521887010621 2316 13 -1.4777205505712203 2316 14 -1.7611128458070269 2316 15 -1.9042463040859587 2316 16 -1.6095971801291356 2316 17 -1.6802241369137647 2316 18 -2.150890908555887 2316 19 -1.9392373503883817 2316 20 -1.8735272703406167 2316 21 -1.62662115034626 2316 22 -1.7253599363915058 2316 23 -1.7031171010149606 2316 24 -1.5641449594576784 2316 25 -1.3785918123998044 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 83516.0 3 3.0 4 744.0 5 3606.0 6 4251.0 7 3859.0 8 3665.0 9 3491.0 10 3404.0 11 3379.0 12 3291.0 13 3332.0 14 3287.0 15 3328.0 16 3597.0 17 3957.0 18 4489.0 19 5619.0 20 7243.0 21 9440.0 22 11873.0 23 14102.0 24 16446.0 25 18623.0 26 21179.0 27 24180.0 28 27601.0 29 33658.0 30 42082.0 31 53360.0 32 68335.0 33 89221.0 34 118713.0 35 164383.0 36 231630.0 37 292888.0 38 629670.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62874948212429 19.284475636894086 13.56276157374674 24.524013307234878 2 15.709669040780666 20.702606802931363 40.86647647995741 22.721247676330556 3 19.159391598381497 24.12901369176534 29.69274775545847 27.018846954394686 4 12.14689559873874 16.713579383213137 38.651166367725246 32.48835865032288 5 13.056421782782463 37.736430416111574 35.95206021498974 13.255087586116218 6 31.536438693287817 37.55309014408764 17.95607906958282 12.954392093041717 7 27.87712274823948 31.742826917122507 21.942657017871177 18.437393316766833 8 24.511410631399023 38.17191736812466 20.376740620557744 16.93993137991858 9 25.531631972095493 16.267573662392177 20.70441019229004 37.49638417322229 10 14.70947388581996 29.818876121945397 34.15452616181419 21.317123830420456 11 33.83583251504793 23.130972470381707 24.615893773571706 18.417301240998654 12 22.974204678574388 24.82987611160425 32.098848739458276 20.09707047036309 13 28.62156992624458 22.26272609771043 26.240061127939484 22.875642848105503 14 20.47879466279257 22.808513889798533 27.89758642794909 28.815105019459807 15 22.51869934431354 31.63271634800255 23.786310219331213 22.062274088352694 16 21.494508656897114 29.005852305570233 28.29003024146032 21.20960879607233 17 21.001338167340876 29.231191355945068 28.4285390358348 21.33893144087925 18 20.73701322223336 28.123818380125066 30.674678276852106 20.464490120789463 19 22.75171654802911 27.319386597677266 28.286796250040936 21.642100604252693 20 22.651970160436033 27.94102543110827 29.091242876883268 20.31576153157243 21 22.146011211172866 27.2525183458037 28.101667683052394 22.49980275997104 22 22.156235905410387 28.406059141580258 28.525638322100665 20.912066630908686 23 21.7215030341522 28.066131121023723 28.618629644882628 21.593736199941453 24 22.316255080627595 27.12011841689356 29.490441150565218 21.073185351913633 25 22.044208298123923 27.768253525613513 28.99043974588689 21.19709843037568 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 617.0 1 617.0 2 721.0 3 825.0 4 825.0 5 825.0 6 1913.0 7 3001.0 8 3001.0 9 3001.0 10 4510.0 11 6019.0 12 6019.0 13 6019.0 14 9226.5 15 12434.0 16 12434.0 17 12434.0 18 20706.0 19 28978.0 20 28978.0 21 28978.0 22 43700.5 23 58423.0 24 58423.0 25 58423.0 26 82089.5 27 105756.0 28 105756.0 29 105756.0 30 135484.5 31 165213.0 32 165213.0 33 165213.0 34 183906.5 35 202600.0 36 202600.0 37 202600.0 38 215877.5 39 229155.0 40 229155.0 41 229155.0 42 240619.5 43 252084.0 44 252084.0 45 252084.0 46 253699.5 47 255315.0 48 255315.0 49 255315.0 50 244848.5 51 234382.0 52 234382.0 53 234382.0 54 207158.0 55 179934.0 56 179934.0 57 179934.0 58 153704.5 59 127475.0 60 127475.0 61 127475.0 62 103710.0 63 79945.0 64 79945.0 65 79945.0 66 61130.5 67 42316.0 68 42316.0 69 42316.0 70 30970.5 71 19625.0 72 19625.0 73 19625.0 74 13535.5 75 7446.0 76 7446.0 77 7446.0 78 5113.0 79 2780.0 80 2780.0 81 2780.0 82 1788.0 83 796.0 84 796.0 85 796.0 86 517.0 87 238.0 88 238.0 89 238.0 90 149.0 91 60.0 92 60.0 93 60.0 94 41.0 95 22.0 96 22.0 97 22.0 98 14.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.542403786756771 3 0.0 4 0.0 5 0.0 6 2.4808416999719664E-4 7 0.00659903892192543 8 0.0 9 0.0 10 0.004167814055952904 11 0.0025800753679708455 12 9.923366799887867E-5 13 0.0 14 4.4655150599495395E-4 15 0.0 16 0.0 17 9.923366799887867E-5 18 0.010072217301886184 19 0.003125860541964678 20 0.0 21 0.00664865575592487 22 0.0021831406959753304 23 0.002728925869969163 24 0.00952643212789235 25 0.006053253747931598 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2015445.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.95154623972242 #Duplication Level Percentage of deduplicated Percentage of total 1 88.2170639877163 68.76656542571018 2 8.49109168350477 13.23787451984889 3 1.7022336417120858 3.980752332981922 4 0.6150577229018737 1.9177880210753513 5 0.3152185757744285 1.2285887692549904 6 0.16902596787800506 0.7905501330453688 7 0.10213398595283624 0.5573051490054772 8 0.0720479225854602 0.4492997575117152 9 0.05315275393226307 0.3729005420327452 >10 0.23479180241885558 3.1735406246002857 >50 0.014226704391662612 0.7562086291308435 >100 0.011126829450733192 1.7414878475288973 >500 0.0017356224563555656 0.9608912788878013 >1k 0.0010285170111735938 1.723701455071952 >5k 6.428231319834961E-5 0.3425455143135517 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6836 0.33918067722016726 No Hit TATCAACGCAGAGTACTTTTTTTTT 4177 0.20724951561565808 No Hit GGTATCAACGCAGAGTACTTTTTTT 4112 0.20402442140569454 No Hit GTCCTACAGTGGACATTTCTAAATT 3669 0.1820441639439429 No Hit CTGTAGGACGTGGAATATGGCAAGA 3324 0.16492635621413634 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2637 0.13083959125652153 No Hit CTTTAGGACGTGAAATATGGCGAGG 2495 0.12379400082860113 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2095 0.1039472672288254 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.961683399943933E-5 0.0 0.0 0.0 6 0.0 4.961683399943933E-5 0.0 0.0 0.0 7 0.0 4.961683399943933E-5 0.0 0.0 0.0 8 0.0 4.961683399943933E-5 0.0 4.961683399943933E-5 0.0 9 0.0 4.961683399943933E-5 0.0 4.961683399943933E-5 0.0 10 0.0 4.961683399943933E-5 0.0 4.961683399943933E-5 0.0 11 0.0 4.961683399943933E-5 0.0 4.961683399943933E-5 0.0 12 0.0 4.961683399943933E-5 0.0 4.961683399943933E-5 0.0 13 0.0 4.961683399943933E-5 0.0 4.961683399943933E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGAC 65 2.0960579E-7 15.949014 3 TACGACA 40 0.0055670817 14.136627 4 ATACCGT 65 5.87836E-5 13.049193 6 ATGCGCC 60 4.3781663E-4 12.56589 11 TGTAGGA 2075 0.0 11.642355 2 GTCCTAC 2035 0.0 11.47048 1 TAGGACC 790 0.0 11.452457 4 GACGTGG 1020 0.0 11.364739 7 CTGTAGG 2050 0.0 11.287105 1 GTCCTAG 245 0.0 11.233323 1 GGTATCA 1420 0.0 11.198163 1 AGGACGT 1880 0.0 11.128833 5 GTAGGAC 2105 0.0 11.05858 3 GTCCTAA 950 0.0 11.051576 1 GGACGTG 1835 0.0 11.042233 6 ACTGCGC 60 0.006235402 10.995426 8 TATGCCG 60 0.006236553 10.995153 5 TAGGACG 2015 0.0 10.850943 4 AATCCCG 360 0.0 10.733897 19 ACGTGGA 1055 0.0 10.719983 8 >>END_MODULE