FastQCFastQC Report
Thu 26 May 2016
SRR1512182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512182_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015445
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89880.4459561039869607No Hit
TATCAACGCAGAGTACTTTTTTTTT59690.29616288214265335No Hit
GGTATCAACGCAGAGTACTTTTTTT56590.28078166360282714No Hit
GTCCTACAGTGGACATTTCTAAATT39080.19390258726980888No Hit
CTGTAGGACGTGGAATATGGCAAGA36560.18139914510195018No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30380.1507359416902967No Hit
CTTTAGGACGTGAAATATGGCGAGG28790.14284686508438582No Hit
GTCCTAAAGTGTGTATTTCTCATTT28440.14111027589440545No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGAC400.005278448614.2505593
AGCACCG852.699071E-713.412295
TAGGACC6850.013.0370074
GGTATCA15550.012.13761
TACACCG802.8661987E-511.8754655
TCCAACG1900.011.49702718
GGCGAGG8600.011.37581319
TGTAGGA21650.011.1020512
GCACCGC600.00588800711.0804676
GTAGGAC20600.011.0683953
CTGTAGG21500.011.0397421
GATATAC7550.010.8579931
GACGTGG9850.010.7992387
ACTGTTC8200.010.7716058
AATCCCG3550.010.70225419
AGGACGT19200.010.5889565
CCAACGA1451.4479156E-810.48082819
GGACGTG18800.010.4068846
GTCCTAC20100.010.1014011
TTAGGAC11700.010.068773
Produced by FastQC (version 0.11.5)