##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512182_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2015445 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.701474364222292 31.0 31.0 34.0 27.0 34.0 2 30.905075554033974 31.0 31.0 34.0 27.0 34.0 3 30.993610840285893 31.0 31.0 34.0 27.0 34.0 4 34.60419857649303 37.0 35.0 37.0 32.0 37.0 5 34.20581658144975 37.0 35.0 37.0 30.0 37.0 6 34.130506662300384 37.0 35.0 37.0 30.0 37.0 7 33.95145092026823 37.0 35.0 37.0 30.0 37.0 8 33.85247476363781 37.0 35.0 37.0 29.0 37.0 9 35.31544299149815 39.0 35.0 39.0 30.0 39.0 10 35.1219393235737 38.0 35.0 39.0 29.0 39.0 11 35.10743880383737 38.0 35.0 39.0 29.0 39.0 12 34.91382895588816 38.0 35.0 39.0 27.0 39.0 13 34.8923076541409 38.0 35.0 39.0 27.0 39.0 14 35.86405979820834 39.0 36.0 41.0 27.0 41.0 15 35.78328855414065 39.0 36.0 41.0 27.0 41.0 16 35.67867046731615 39.0 36.0 41.0 27.0 41.0 17 35.61984772593645 39.0 36.0 41.0 27.0 41.0 18 35.5507731543158 39.0 35.0 41.0 27.0 41.0 19 35.577512162326435 39.0 35.0 41.0 27.0 41.0 20 35.443506024724066 39.0 35.0 41.0 27.0 41.0 21 35.33062127718692 39.0 35.0 40.0 27.0 41.0 22 35.42806377747842 39.0 35.0 41.0 27.0 41.0 23 35.317838492243645 39.0 35.0 41.0 27.0 41.0 24 35.1819121831655 39.0 35.0 41.0 26.0 41.0 25 34.72287162388455 39.0 34.0 40.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35451061044132715 1101 2 0.3639020173277885 1101 3 0.46357241594525433 1101 4 0.4314375865196851 1101 5 0.41706912849625155 1101 6 0.3678652590020093 1101 7 0.2845814773585573 1101 8 0.1900166224938431 1101 9 0.27392827157243715 1101 10 0.4604679613967875 1101 11 0.33743703362613076 1101 12 0.5808748544574058 1101 13 0.4431461415626643 1101 14 0.40170239821431863 1101 15 0.3594800508168916 1101 16 0.6499809626058308 1101 17 0.3864053910807854 1101 18 0.275380470503265 1101 19 0.3213749923480904 1101 20 0.22335640145679747 1101 21 0.23156720713078016 1101 22 0.21763534633601012 1101 23 0.25135826782873494 1101 24 0.11950190439823416 1101 25 0.2957953745988391 1102 1 0.350479173660581 1102 2 0.3762719910058472 1102 3 0.4354514075531455 1102 4 0.2697850350434621 1102 5 0.446091901970739 1102 6 0.4399877718239793 1102 7 0.4039933658142374 1102 8 0.43635417838681434 1102 9 0.5165067058828114 1102 10 0.45237690767647365 1102 11 0.529858691389542 1102 12 0.5357589782023382 1102 13 0.5933522473381458 1102 14 0.5174592363670172 1102 15 0.7040801464620259 1102 16 0.7282478660974405 1102 17 0.7634333162231215 1102 18 0.8003310715218745 1102 19 0.7316398802608077 1102 20 0.7925325531185052 1102 21 0.7825724575686905 1102 22 0.7319101313437173 1102 23 0.8026475519466771 1102 24 1.0363768531298732 1102 25 0.6205075324121907 1103 1 0.7132939482087473 1103 2 0.5590641102032272 1103 3 0.6318316443246133 1103 4 0.60619099362588 1103 5 0.5922577813827274 1103 6 0.7864420164320762 1103 7 0.7773954693415774 1103 8 0.8011055940781944 1103 9 0.8659142947284053 1103 10 0.8479508048095994 1103 11 0.8580160329615723 1103 12 1.0076400209917864 1103 13 0.9343037506273504 1103 14 0.7924813100553365 1103 15 1.1908501399699176 1103 16 1.1567773644256292 1103 17 0.9345417933235822 1103 18 1.0583731581515252 1103 19 1.0879936446891207 1103 20 0.8724854089739722 1103 21 1.0846817747291269 1103 22 1.2619625131308752 1103 23 1.4821593093017569 1103 24 1.0367757073888484 1103 25 1.3010250029256554 1104 1 0.23326248212956102 1104 2 0.19126165989288424 1104 3 0.24023534106238031 1104 4 0.23987865382591878 1104 5 0.3018931623981942 1104 6 0.31997634794638685 1104 7 0.3780858696600262 1104 8 0.32535859062198114 1104 9 0.5482174685058325 1104 10 0.5268572637804851 1104 11 0.5148862322256775 1104 12 0.7202424595076167 1104 13 0.7628030882390036 1104 14 0.8465065039803221 1104 15 0.7941474438703224 1104 16 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-0.6476608286209284 2314 25 -0.7314426053858085 2315 1 -0.3209391080318049 2315 2 -0.28752476498569735 2315 3 -0.3575576246277343 2315 4 -0.5117555698933316 2315 5 -0.3626190183945326 2315 6 -0.4029527214567139 2315 7 -0.43666914365659437 2315 8 -0.2484713091116859 2315 9 -0.40379177479669437 2315 10 -0.4160819889220022 2315 11 -0.43022774239440764 2315 12 -0.3925767477616944 2315 13 -0.46266397270412796 2315 14 -0.6765988148352804 2315 15 -0.4762671339144191 2315 16 -0.8453883344050155 2315 17 -0.5032500490119531 2315 18 -0.6207205893625982 2315 19 -0.8590894234836597 2315 20 -1.099010494295456 2315 21 -0.6802936647744957 2315 22 -0.6745978241533521 2315 23 -0.6424931646462184 2315 24 -0.5478078579599668 2315 25 -0.4865848729792077 2316 1 -0.8531010243183239 2316 2 -0.8418730168922366 2316 3 -0.8104674863820982 2316 4 -0.757779049017195 2316 5 -0.9376340356317101 2316 6 -0.9758653254891669 2316 7 -0.8504469146633156 2316 8 -0.9325741488930035 2316 9 -1.0696229897564749 2316 10 -1.081694909839925 2316 11 -1.1434085238130791 2316 12 -1.4988247889916266 2316 13 -1.2679371993642903 2316 14 -1.5187604699524613 2316 15 -1.6951333542716966 2316 16 -1.3973932443886454 2316 17 -1.4304406068696665 2316 18 -1.4205878282803113 2316 19 -1.3423582624988768 2316 20 -1.5093171071032003 2316 21 -1.5349252854173159 2316 22 -1.6846615494728496 2316 23 -1.5238162920620368 2316 24 -1.4058153099918655 2316 25 -1.3393095221417184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32963.0 3 2.0 4 570.0 5 5382.0 6 6686.0 7 5580.0 8 4281.0 9 4026.0 10 3822.0 11 3597.0 12 3458.0 13 3296.0 14 3278.0 15 3371.0 16 3361.0 17 3617.0 18 3937.0 19 4335.0 20 5298.0 21 6717.0 22 8870.0 23 11040.0 24 13511.0 25 16405.0 26 18933.0 27 21562.0 28 25291.0 29 31309.0 30 39714.0 31 51016.0 32 65635.0 33 85235.0 34 110102.0 35 145410.0 36 207683.0 37 355772.0 38 700380.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.494571999778806 20.115310442953312 14.352777419359661 24.03734013790822 2 14.75847765629923 20.874844017078114 41.03624757807829 23.330430748544366 3 18.958889972189766 24.681993306689094 30.272371610239922 26.08674511088122 4 12.687173304158636 17.453912163318773 38.31074526965509 31.548169262867503 5 13.006821480118244 37.86254002854857 35.966234070474066 13.164404420859116 6 31.593519049142994 37.908551213255635 17.67445899044628 12.823470747155094 7 27.844818389983356 31.894097829511598 21.93262530111216 18.32845847939289 8 24.648402710071473 38.30761940911312 20.154854138912253 16.889123741903152 9 25.535353234645452 16.134302846269684 20.61435563858106 37.71598828050381 10 14.70623113009782 30.07509507825815 34.104527784186615 21.11414600745741 11 34.08145595637688 23.048110963087556 24.439218137929835 18.431214942605727 12 22.985345668078267 25.098873945952384 31.90347541113749 20.01230497483186 13 28.724772940963412 22.269374753466355 26.321631203034567 22.684221102535666 14 20.48917236640047 22.877825988801483 27.904060889778687 28.728940755019362 15 22.519046662151535 31.75120134759321 23.74959376217163 21.980158228083624 16 21.451639712321597 29.220048177945813 28.21089139123122 21.11742071850137 17 21.06889545484992 29.298690859834924 28.396210266219125 21.236203419096032 18 20.847257057374428 28.182609795851537 30.586892720962368 20.383240425811668 19 22.84855205674181 27.375591990850655 28.394175976025142 21.381679976382387 20 22.66978260384183 27.937701103230307 29.109253787625068 20.283262505302798 21 22.20273934540511 27.233935929782255 28.250287157426772 22.313037567385862 22 22.152725576733673 28.34436067468971 28.673717218777988 20.82919652979863 23 21.67948021404702 28.050083232239032 28.856555252065917 21.413881301648026 24 22.333380469325633 27.205852801738573 29.507230413134565 20.953536315801223 25 22.058680507313973 27.817468193636742 29.15536986682016 20.968481432229126 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 633.0 1 633.0 2 722.5 3 812.0 4 812.0 5 812.0 6 2030.0 7 3248.0 8 3248.0 9 3248.0 10 4672.0 11 6096.0 12 6096.0 13 6096.0 14 9202.0 15 12308.0 16 12308.0 17 12308.0 18 20464.0 19 28620.0 20 28620.0 21 28620.0 22 44252.5 23 59885.0 24 59885.0 25 59885.0 26 83925.5 27 107966.0 28 107966.0 29 107966.0 30 137103.0 31 166240.0 32 166240.0 33 166240.0 34 184781.0 35 203322.0 36 203322.0 37 203322.0 38 216570.0 39 229818.0 40 229818.0 41 229818.0 42 240829.5 43 251841.0 44 251841.0 45 251841.0 46 253387.0 47 254933.0 48 254933.0 49 254933.0 50 244063.0 51 233193.0 52 233193.0 53 233193.0 54 206267.5 55 179342.0 56 179342.0 57 179342.0 58 152710.0 59 126078.0 60 126078.0 61 126078.0 62 102641.0 63 79204.0 64 79204.0 65 79204.0 66 60537.5 67 41871.0 68 41871.0 69 41871.0 70 30444.5 71 19018.0 72 19018.0 73 19018.0 74 13198.5 75 7379.0 76 7379.0 77 7379.0 78 5000.5 79 2622.0 80 2622.0 81 2622.0 82 1680.5 83 739.0 84 739.0 85 739.0 86 478.0 87 217.0 88 217.0 89 217.0 90 135.5 91 54.0 92 54.0 93 54.0 94 30.0 95 6.0 96 6.0 97 6.0 98 3.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40527030010742043 2 0.0 3 0.0 4 0.0 5 0.031407455921645094 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.006202104249929916 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2015445.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.59042715786227 #Duplication Level Percentage of deduplicated Percentage of total 1 87.11058954966724 65.8472667403256 2 9.167438198656702 13.85941138759527 3 1.9379100599609451 4.39462347677899 4 0.7058635656229684 2.1342611376244767 5 0.3306270576669305 1.2496120259495218 6 0.18554629579007553 0.841531425379852 7 0.11526099640006304 0.6098839566765112 8 0.09241222009795264 0.5588383353448498 9 0.056245599794337985 0.38264660227837605 >10 0.26416253572732 3.4453201184365034 >50 0.017938392506758428 0.9285649944301873 >100 0.012727064235936305 1.952272565423081 >500 0.0020326476010527014 1.1745204045066695 >1k 0.0010491084392529875 1.5994334073270766 >5k 1.9670783235993517E-4 1.0218134219229937 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8988 0.4459561039869607 No Hit TATCAACGCAGAGTACTTTTTTTTT 5969 0.29616288214265335 No Hit GGTATCAACGCAGAGTACTTTTTTT 5659 0.28078166360282714 No Hit GTCCTACAGTGGACATTTCTAAATT 3908 0.19390258726980888 No Hit CTGTAGGACGTGGAATATGGCAAGA 3656 0.18139914510195018 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3038 0.1507359416902967 No Hit CTTTAGGACGTGAAATATGGCGAGG 2879 0.14284686508438582 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2844 0.14111027589440545 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.961683399943933E-5 0.0 9 0.0 0.0 0.0 4.961683399943933E-5 0.0 10 0.0 0.0 0.0 4.961683399943933E-5 0.0 11 0.0 0.0 0.0 4.961683399943933E-5 0.0 12 0.0 0.0 0.0 4.961683399943933E-5 0.0 13 0.0 0.0 0.0 4.961683399943933E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGGAC 40 0.0052784486 14.250559 3 AGCACCG 85 2.699071E-7 13.41229 5 TAGGACC 685 0.0 13.037007 4 GGTATCA 1555 0.0 12.1376 1 TACACCG 80 2.8661987E-5 11.875465 5 TCCAACG 190 0.0 11.497027 18 GGCGAGG 860 0.0 11.375813 19 TGTAGGA 2165 0.0 11.102051 2 GCACCGC 60 0.005888007 11.080467 6 GTAGGAC 2060 0.0 11.068395 3 CTGTAGG 2150 0.0 11.039742 1 GATATAC 755 0.0 10.857993 1 GACGTGG 985 0.0 10.799238 7 ACTGTTC 820 0.0 10.771605 8 AATCCCG 355 0.0 10.702254 19 AGGACGT 1920 0.0 10.588956 5 CCAACGA 145 1.4479156E-8 10.480828 19 GGACGTG 1880 0.0 10.406884 6 GTCCTAC 2010 0.0 10.101401 1 TTAGGAC 1170 0.0 10.06877 3 >>END_MODULE