FastQCFastQC Report
Thu 26 May 2016
SRR1512181_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512181_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2417409
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63780.26383619817747017No Hit
GTCCTACAGTGGACATTTCTAAATT55010.2275576867629764No Hit
CTGTAGGACGTGGAATATGGCAAGA51180.21171427755915526No Hit
GTCCTAAAGTGTGTATTTCTCATTT42830.17717316349860532No Hit
CTTTAGGACGTGAAATATGGCGAGG39610.1638531171183693No Hit
GGTATCAACGCAGAGTACTTTTTTT38310.1584754586418765No Hit
TATCAACGCAGAGTACTTTTTTTTT37470.15500066393398884No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA400.00329277515.2992371
TCCAACG3700.014.51911118
GTCGGAA400.005567982314.13644413
ATCGCGC552.1003518E-413.7066518
TCGCGCG552.1003518E-413.7066519
ACTATCG1206.002665E-1113.34970611
CCAACGA2500.013.19428819
CCAACGT1600.012.95867619
TAGGACC8850.012.03253654
ATCGGTC1451.1277734E-1011.69912714
CTATCGG1407.7852746E-1011.443789512
CGTTTCC2750.011.30915614
GGCGAGG12600.011.29443119
TATCGGT1605.2750693E-1111.19135213
TGTAGGA28550.011.1462042
CTGTAGG28450.011.0419811
ATCCCGT2650.011.02350910
AGGACGT30550.011.0123245
GGACGTG30300.010.8543756
GCACCGT804.06397E-410.6016756
Produced by FastQC (version 0.11.5)