##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512181_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2417409 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.049403721091466 31.0 30.0 34.0 23.0 34.0 2 26.148544164433904 31.0 26.0 34.0 2.0 34.0 3 27.81089381234206 31.0 26.0 34.0 16.0 34.0 4 32.32803882173021 35.0 32.0 37.0 24.0 37.0 5 32.747141671103236 35.0 33.0 37.0 26.0 37.0 6 32.94872981775116 35.0 35.0 37.0 26.0 37.0 7 32.939301541443754 36.0 35.0 37.0 26.0 37.0 8 32.9445488951187 37.0 35.0 37.0 26.0 37.0 9 34.41106366361671 39.0 35.0 39.0 27.0 39.0 10 34.27234737688161 38.0 35.0 39.0 26.0 39.0 11 34.28895069059477 38.0 35.0 39.0 26.0 39.0 12 34.16471106047839 38.0 35.0 39.0 26.0 39.0 13 34.16201147592319 38.0 35.0 39.0 26.0 39.0 14 35.17238663378849 39.0 35.0 41.0 25.0 41.0 15 35.10127537375761 39.0 35.0 41.0 25.0 41.0 16 35.04258609114138 39.0 35.0 41.0 25.0 41.0 17 35.023226106959974 39.0 35.0 41.0 25.0 41.0 18 34.976534794070844 39.0 35.0 41.0 25.0 41.0 19 34.996490043678996 39.0 35.0 41.0 25.0 41.0 20 34.90229994179719 39.0 34.0 41.0 24.0 41.0 21 34.825887965172626 39.0 34.0 41.0 24.0 41.0 22 34.70878076486023 39.0 34.0 41.0 22.0 41.0 23 34.583388661165735 39.0 34.0 41.0 21.0 41.0 24 34.46216051979619 39.0 34.0 41.0 19.0 41.0 25 34.322706666517746 39.0 34.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4047427236407799 1101 2 3.366958117548318 1101 3 1.9433531179497514 1101 4 0.8487144300705438 1101 5 0.34891219961113507 1101 6 0.3849528853449442 1101 7 0.2652603202877941 1101 8 0.1792580026890107 1101 9 0.060334971844618224 1101 10 0.10767228735923595 1101 11 0.31222841737340445 1101 12 0.19048168995546888 1101 13 0.09884713810969004 1101 14 0.27251326017790944 1101 15 0.3990666754228016 1101 16 0.48437212461158197 1101 17 0.1658369474130552 1101 18 0.24646298742261052 1101 19 -0.06236420260857045 1101 20 0.0888659084028589 1101 21 0.2604568646696066 1101 22 0.22572195392882577 1101 23 0.12793996352648662 1101 24 0.1110544428260738 1101 25 0.17480391831631437 1102 1 1.414527604074415 1102 2 4.3213369805307025 1102 3 2.708773770242786 1102 4 1.7730715977970775 1102 5 1.3369193880436967 1102 6 1.332033336241608 1102 7 1.2290221632110345 1102 8 1.1688788060826596 1102 9 1.0609224991576554 1102 10 1.1698182846960634 1102 11 1.1670793000706325 1102 12 1.313570310759836 1102 13 1.3175289161314367 1102 14 1.4253246724449937 1102 15 1.438143739648325 1102 16 1.3059814528927092 1102 17 1.401788226676679 1102 18 1.525939003836136 1102 19 1.5126619102552468 1102 20 1.4172488699168113 1102 21 1.7422740678339892 1102 22 1.5501227643246978 1102 23 1.6076753143796054 1102 24 1.5530041645313233 1102 25 1.6672398678498723 1103 1 0.9947679731409309 1103 2 4.029391351671546 1103 3 2.4607263506972785 1103 4 1.5693813043802791 1103 5 1.1931167166980217 1103 6 0.9749116684535153 1103 7 0.7776827729440114 1103 8 0.9277681121498844 1103 9 0.9935072658702921 1103 10 0.998794541466431 1103 11 0.9491252252090163 1103 12 0.9780744240968602 1103 13 1.025487563432769 1103 14 1.0790701697071015 1103 15 1.2474322977684906 1103 16 1.2041708782894531 1103 17 1.2308113230733753 1103 18 1.0543067172073606 1103 19 1.3241705179659462 1103 20 1.267359277607433 1103 21 1.299190347578083 1103 22 1.3505450644477506 1103 23 1.4470849120420866 1103 24 1.4430252795374727 1103 25 1.5074541033788407 1104 1 1.2460566376882873 1104 2 4.058172264312372 1104 3 2.555355679352296 1104 4 1.607296851204154 1104 5 1.387328810926384 1104 6 1.0731814237345105 1104 7 1.0542148396100401 1104 8 0.9885605440168419 1104 9 1.1413571308251846 1104 10 1.1201318901262738 1104 11 0.9690293472387452 1104 12 0.9945026377167565 1104 13 1.0987512876087564 1104 14 1.2069830827640189 1104 15 0.9390323886592711 1104 16 1.1584394251362014 1104 17 1.2788989648043412 1104 18 1.1319626351046566 1104 19 1.2327184934696973 1104 20 1.192350959477892 1104 21 1.2170048300502572 1104 22 1.2249461037897333 1104 23 1.2165805050933685 1104 24 1.4509116409773526 1104 25 1.3026088205882616 1105 1 0.9792940517976234 1105 2 3.6350521099254713 1105 3 2.3045480134648884 1105 4 1.396068546123466 1105 5 1.032241635532884 1105 6 0.7142009399210139 1105 7 0.4587947876956662 1105 8 0.42564174805192323 1105 9 0.6445116189940734 1105 10 0.6399435147951493 1105 11 0.7700205805046707 1105 12 0.4493428681747105 1105 13 0.6689589972767678 1105 14 0.8575393609014696 1105 15 0.8475478249211648 1105 16 0.620182566069289 1105 17 0.6473320309272736 1105 18 0.7003692961605452 1105 19 0.7484211443549569 1105 20 0.7348060083587455 1105 21 0.6536180096682074 1105 22 0.9205399291051037 1105 23 0.685428731538039 1105 24 0.745843109215997 1105 25 0.7756809584040028 1106 1 0.24562512467259978 1106 2 2.9785403807391546 1106 3 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0.778997879183926 1107 15 0.8271194465223033 1107 16 0.8805045803636702 1107 17 0.7207213972155699 1107 18 0.6426528425924758 1107 19 0.5688122733274312 1107 20 0.7857347701634936 1107 21 0.8423923052983753 1107 22 0.9288890648467785 1107 23 0.9259997205986039 1107 24 0.8462179857106591 1107 25 1.0472499338965093 1108 1 0.8950543786322527 1108 2 3.3186275352584893 1108 3 2.081186378824558 1108 4 1.130692610382475 1108 5 0.7466175704396036 1108 6 0.6005930363562513 1108 7 0.5131154437008831 1108 8 0.40156046987957694 1108 9 0.6108849276106909 1108 10 0.47992052224270054 1108 11 0.4785856400008157 1108 12 0.630756609812174 1108 13 0.4950495375433519 1108 14 0.6648611039638723 1108 15 0.6588780809010188 1108 16 0.5826523132596648 1108 17 0.6456554767400178 1108 18 0.6306137630561963 1108 19 0.6918623018581158 1108 20 0.5908878137913618 1108 21 0.633623165472784 1108 22 0.6508643193144152 1108 23 0.7217279317209133 1108 24 0.6483792144478073 1108 25 0.540956531152986 1109 1 0.7442601113539098 1109 2 3.335476537954115 1109 3 1.866659228380989 1109 4 1.0232506086212823 1109 5 0.7752473392157242 1109 6 0.4850226290130806 1109 7 0.5387388791039385 1109 8 0.2306824497989055 1109 9 0.36413717428538206 1109 10 0.33934425066520646 1109 11 0.31959067362883786 1109 12 0.337642566797534 1109 13 0.3093575002122151 1109 14 0.3735397862223522 1109 15 0.23218562442133361 1109 16 0.3503786438640475 1109 17 0.3578773101496182 1109 18 0.3432685884558282 1109 19 0.33369871087019476 1109 20 0.37032338604449677 1109 21 0.37948993775975737 1109 22 0.3027118415312131 1109 23 0.4520223739649367 1109 24 0.40223903425238206 1109 25 0.33638358200070684 1110 1 0.782017437133451 1110 2 3.600557452873719 1110 3 2.018177102429185 1110 4 1.2297527600411442 1110 5 0.765102438581998 1110 6 0.4433270354328087 1110 7 0.5915431500157737 1110 8 0.4444188665246642 1110 9 0.5712585782214248 1110 10 0.4148529621521888 1110 11 0.42434830012452807 1110 12 0.23364675454836004 1110 13 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-1.9202335637568382 2315 16 -1.8139701130468424 2315 17 -1.736643128586806 2315 18 -1.6792324816046929 2315 19 -1.9908699560549934 2315 20 -1.9578428514369577 2315 21 -1.7595572478456276 2315 22 -1.6966256110513669 2315 23 -1.6052998973033397 2315 24 -1.7359438984951865 2315 25 -1.5460431991566281 2316 1 -1.0357260911921387 2316 2 0.1314764284962351 2316 3 -0.08317118391516232 2316 4 -0.7871412649951921 2316 5 -0.9927008007924236 2316 6 -0.8629170424116452 2316 7 -0.9894616477341245 2316 8 -1.0225915650441628 2316 9 -1.1079648923082175 2316 10 -1.0047227721236354 2316 11 -1.5315587061338434 2316 12 -1.0685714723278963 2316 13 -1.180577415555625 2316 14 -1.2983461010561967 2316 15 -1.6206453740505822 2316 16 -1.3767141980226043 2316 17 -1.3600621164351097 2316 18 -1.7242986093216004 2316 19 -1.7205238429261982 2316 20 -1.8142563028934902 2316 21 -1.6401018707580803 2316 22 -1.4324490543527375 2316 23 -1.32128502553185 2316 24 -1.248693302054079 2316 25 -1.1176085341513868 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 101689.0 3 1.0 4 964.0 5 4664.0 6 5305.0 7 5005.0 8 4444.0 9 4364.0 10 4233.0 11 4183.0 12 4060.0 13 3874.0 14 4161.0 15 4240.0 16 4429.0 17 4894.0 18 5549.0 19 6728.0 20 8895.0 21 11478.0 22 14377.0 23 17059.0 24 20068.0 25 22808.0 26 25647.0 27 29139.0 28 33704.0 29 40443.0 30 50851.0 31 64478.0 32 81894.0 33 106898.0 34 142021.0 35 194637.0 36 275869.0 37 345074.0 38 759282.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.15968419080098 19.485614556742366 13.620078356620663 24.734622895835997 2 15.26918895879337 20.99605824696757 41.43796977332681 22.296783020912244 3 18.780810363492485 24.534118967870143 30.094576465960042 26.590494202677327 4 11.942207545351241 17.2069351938377 39.27146792288769 31.57938933792337 5 12.765196125272968 38.05884730304223 36.329599170020465 12.846357401664344 6 30.817909776016112 37.99159098637134 18.551621035109534 12.638878202503012 7 27.34070125577364 31.935413809579956 22.503521585613314 18.22036334903309 8 23.987128367603496 38.50750948639639 20.962898706838605 16.542463439161516 9 25.552357917092227 16.27072621968397 21.11268717871076 37.06422868451305 10 14.642119310937677 29.85470650482312 34.69967079163071 20.80350339260849 11 33.376245426283795 23.049874966130197 25.355007249689226 18.218872357896785 12 22.986167817900675 24.894618446384264 32.47067310993685 19.64854062577821 13 28.446572342536992 22.557953577570032 26.424531388772028 22.570942691120948 14 20.132348640255678 23.253590733526845 28.29441346767585 28.319647158541628 15 22.175891626117053 32.19566899932945 23.872625608657867 21.755813765895635 16 21.054443000749977 29.039479872872153 29.09999921403453 20.80607791234334 17 20.668261488446092 29.49404051531248 28.86080002250345 20.976897973737977 18 20.388059516249474 28.28531889762965 31.388512528158103 19.93810905796277 19 22.689912023242048 27.4668510012853 28.290730773415273 21.552506202057383 20 22.480267095886546 28.33657854339088 29.287844961278793 19.895309399443782 21 21.925069976444227 27.690731834048886 28.009483535367995 22.37471465413889 22 21.963809324458097 29.080282936406327 28.504945264736826 20.450962474398757 23 21.409530459876624 28.586125929946256 28.737490402689897 21.266853207487227 24 22.21750136833513 27.436536347656 29.66459551268818 20.68136677132069 25 21.894486188270424 28.166512704275963 29.169012645324845 20.76998846212877 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 353.0 1 353.0 2 545.0 3 737.0 4 737.0 5 737.0 6 1883.0 7 3029.0 8 3029.0 9 3029.0 10 4757.0 11 6485.0 12 6485.0 13 6485.0 14 10460.0 15 14435.0 16 14435.0 17 14435.0 18 24520.5 19 34606.0 20 34606.0 21 34606.0 22 54018.5 23 73431.0 24 73431.0 25 73431.0 26 104545.0 27 135659.0 28 135659.0 29 135659.0 30 175844.5 31 216030.0 32 216030.0 33 216030.0 34 237287.0 35 258544.0 36 258544.0 37 258544.0 38 272518.0 39 286492.0 40 286492.0 41 286492.0 42 298280.5 43 310069.0 44 310069.0 45 310069.0 46 308266.5 47 306464.0 48 306464.0 49 306464.0 50 289391.0 51 272318.0 52 272318.0 53 272318.0 54 237103.5 55 201889.0 56 201889.0 57 201889.0 58 170180.5 59 138472.0 60 138472.0 61 138472.0 62 111465.5 63 84459.0 64 84459.0 65 84459.0 66 64074.0 67 43689.0 68 43689.0 69 43689.0 70 31462.5 71 19236.0 72 19236.0 73 19236.0 74 13263.0 75 7290.0 76 7290.0 77 7290.0 78 4931.0 79 2572.0 80 2572.0 81 2572.0 82 1688.0 83 804.0 84 804.0 85 804.0 86 524.0 87 244.0 88 244.0 89 244.0 90 158.5 91 73.0 92 73.0 93 73.0 94 47.5 95 22.0 96 22.0 97 22.0 98 14.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.606697914999075 3 0.0 4 0.0 5 0.0 6 1.2409981099598786E-4 7 0.007197789037767295 8 0.0 9 0.0 10 0.003929827348206282 11 0.002647462634581074 12 1.2409981099598786E-4 13 0.0 14 2.481996219919757E-4 15 0.0 16 0.0 17 8.273320733065856E-5 18 0.010507117330993639 19 0.002730195841911733 20 0.0 21 0.006908222812109991 22 0.001902863768605147 23 0.0026888292382464037 24 0.00984525167234837 25 0.006287723757130051 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2417409.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.30153271298902 #Duplication Level Percentage of deduplicated Percentage of total 1 86.70814188778179 65.29255982845294 2 9.652372516996246 14.53676889693098 3 1.9676569334734249 4.445027488316662 4 0.6928487533907676 2.0869029227443416 5 0.30142152814840184 1.134875153113301 6 0.16106228943990716 0.7276942354252844 7 0.10721024619260987 0.5651167102588293 8 0.0680197895532184 0.4097595526537844 9 0.047762012679774125 0.3236897484219787 >10 0.2559281876972713 3.3777074514349845 >50 0.019718958454614518 1.0399424098867325 >100 0.0140795743504509 2.1303174288942577 >500 0.001944209771386714 0.9905557675907629 >1k 0.001666465518316583 2.2281125827414137 >5k 1.6664655183165828E-4 0.7109698231335688 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6378 0.26383619817747017 No Hit GTCCTACAGTGGACATTTCTAAATT 5501 0.2275576867629764 No Hit CTGTAGGACGTGGAATATGGCAAGA 5118 0.21171427755915526 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4283 0.17717316349860532 No Hit CTTTAGGACGTGAAATATGGCGAGG 3961 0.1638531171183693 No Hit GGTATCAACGCAGAGTACTTTTTTT 3831 0.1584754586418765 No Hit TATCAACGCAGAGTACTTTTTTTTT 3747 0.15500066393398884 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.136660366532928E-5 0.0 4 0.0 0.0 0.0 4.136660366532928E-5 0.0 5 0.0 0.0 0.0 4.136660366532928E-5 0.0 6 0.0 0.0 0.0 4.136660366532928E-5 0.0 7 0.0 0.0 0.0 4.136660366532928E-5 0.0 8 0.0 0.0 0.0 4.136660366532928E-5 0.0 9 0.0 0.0 0.0 1.2409981099598786E-4 0.0 10 0.0 0.0 0.0 2.0683301832664642E-4 0.0 11 0.0 0.0 0.0 2.89566225657305E-4 0.0 12 0.0 0.0 0.0 3.3093282932263426E-4 0.0 13 0.0 0.0 0.0 3.3093282932263426E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATA 40 0.003292775 15.299237 1 TCCAACG 370 0.0 14.519111 18 GTCGGAA 40 0.0055679823 14.136444 13 ATCGCGC 55 2.1003518E-4 13.706651 8 TCGCGCG 55 2.1003518E-4 13.706651 9 ACTATCG 120 6.002665E-11 13.349706 11 CCAACGA 250 0.0 13.194288 19 CCAACGT 160 0.0 12.958676 19 TAGGACC 885 0.0 12.0325365 4 ATCGGTC 145 1.1277734E-10 11.699127 14 CTATCGG 140 7.7852746E-10 11.4437895 12 CGTTTCC 275 0.0 11.309156 14 GGCGAGG 1260 0.0 11.294431 19 TATCGGT 160 5.2750693E-11 11.191352 13 TGTAGGA 2855 0.0 11.146204 2 CTGTAGG 2845 0.0 11.041981 1 ATCCCGT 265 0.0 11.023509 10 AGGACGT 3055 0.0 11.012324 5 GGACGTG 3030 0.0 10.854375 6 GCACCGT 80 4.06397E-4 10.601675 6 >>END_MODULE