FastQCFastQC Report
Thu 26 May 2016
SRR1512181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512181_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2417409
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83190.34412877589187435No Hit
GTCCTACAGTGGACATTTCTAAATT59510.2461726584123746No Hit
CTGTAGGACGTGGAATATGGCAAGA58060.24017450088090184No Hit
TATCAACGCAGAGTACTTTTTTTTT54800.2266889880860045No Hit
GGTATCAACGCAGAGTACTTTTTTT52740.21816746773094664No Hit
CTTTAGGACGTGAAATATGGCGAGG45800.18945904478720813No Hit
GTCCTAAAGTGTGTATTTCTCATTT44550.18428821932904196No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27570.11404772630531283No Hit
TTGTAGAACAGTGTATATCAATGAG25550.10569167236491632No Hit
GTCCTACAGTGTGCATTTCTCATTT24200.10010718087009687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGA250.006043201318.99445716
TACCGTA456.779232E-414.7734667
TCCAACG3200.012.76190218
TAGGACC8750.012.594784
GGACCGT550.003076008312.0873816
TAGGACT4100.011.8175374
CCAACGA2100.011.75895919
TGTAGGA29550.011.3168552
AGGACGT30050.011.2866335
GTAGGAC29500.011.2394243
GGACGTG29300.011.1179166
GTACCGT600.00589003611.0800996
TTAGGAC18250.011.036053
GACGTGG14850.010.9362027
AAGGCGA700.001495557310.85397517
TAGGACG32250.010.8407354
GGCGAGG13550.010.72425119
CTGTAGG30450.010.5549891
GCCGGTT2700.010.55247611
GACGTGA15850.010.5458187
Produced by FastQC (version 0.11.5)