##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512181_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2417409 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.88339333559195 31.0 31.0 34.0 27.0 34.0 2 31.08805419356013 31.0 31.0 34.0 27.0 34.0 3 31.179971200570527 31.0 31.0 34.0 27.0 34.0 4 34.781062286108806 37.0 35.0 37.0 32.0 37.0 5 34.40247471569767 37.0 35.0 37.0 30.0 37.0 6 34.33695870247856 37.0 35.0 37.0 30.0 37.0 7 34.167325429830036 37.0 35.0 37.0 30.0 37.0 8 34.07112036068369 37.0 35.0 37.0 30.0 37.0 9 35.57325094760547 39.0 35.0 39.0 30.0 39.0 10 35.381596577161744 39.0 35.0 39.0 30.0 39.0 11 35.36629258846972 39.0 35.0 39.0 30.0 39.0 12 35.19672757071724 38.0 35.0 39.0 29.0 39.0 13 35.177793248887546 38.0 35.0 39.0 29.0 39.0 14 36.18331527681083 39.0 36.0 41.0 29.0 41.0 15 36.117127469948194 39.0 36.0 41.0 28.0 41.0 16 36.02242897250734 39.0 36.0 41.0 28.0 41.0 17 35.956920818942926 39.0 36.0 41.0 27.0 41.0 18 35.903698546667115 39.0 36.0 41.0 27.0 41.0 19 35.93393050162385 39.0 36.0 41.0 27.0 41.0 20 35.81359008756896 39.0 36.0 41.0 27.0 41.0 21 35.701230118693196 39.0 36.0 41.0 27.0 41.0 22 35.79507067277403 39.0 36.0 41.0 27.0 41.0 23 35.6915118624941 39.0 36.0 41.0 27.0 41.0 24 35.57269498045221 39.0 36.0 41.0 27.0 41.0 25 35.126516861648156 39.0 35.0 40.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21994950445119343 1101 2 0.1709061576929578 1101 3 0.2555372384636918 1101 4 0.19643343635471666 1101 5 0.30783976279960257 1101 6 0.19752921161575898 1101 7 0.08777992178200833 1101 8 -0.15076823567972042 1101 9 -0.0055485225259204185 1101 10 0.19460588137282997 1101 11 0.09512839367413051 1101 12 0.1648729407469247 1101 13 0.08082612292304958 1101 14 0.05911900650488633 1101 15 0.03891860849718398 1101 16 0.028026671162336925 1101 17 0.06682666595895626 1101 18 0.03649793821777081 1101 19 0.02923040563184287 1101 20 -0.03721705726926672 1101 21 0.006408193899289927 1101 22 -0.013411546610626601 1101 23 -0.17280624694573277 1101 24 -0.33405138623282227 1101 25 -0.21443296839791515 1102 1 0.3742818152529459 1102 2 0.40918136060712484 1102 3 0.28399516838520356 1102 4 0.3138151477486488 1102 5 0.4277440204814411 1102 6 0.5123932259072745 1102 7 0.4704563036301508 1102 8 0.6200315210851315 1102 9 0.824575393559897 1102 10 0.6641738480414645 1102 11 0.792818920596325 1102 12 0.8124989250103525 1102 13 0.7891191029511972 1102 14 0.8556865590530833 1102 15 1.023127332957742 1102 16 1.0243804716144709 1102 17 0.9306826800642654 1102 18 1.067390741153659 1102 19 1.0327780697307034 1102 20 0.9326388540333141 1102 21 0.8593236696868232 1102 22 0.8203292799427331 1102 23 0.8552957527613714 1102 24 0.8412711088476783 1102 25 0.9421289978886094 1103 1 0.41284328111020585 1103 2 0.39604154432254646 1103 3 0.33724085709499363 1103 4 0.4532821797148401 1103 5 0.3694112137841188 1103 6 0.5163725495579428 1103 7 0.5681717566176587 1103 8 0.45682906609392404 1103 9 0.5902330256309156 1103 10 0.6342076394861706 1103 11 0.6914596420752659 1103 12 0.768488635162285 1103 13 0.7857369845361575 1103 14 0.7409627455640191 1103 15 0.8275373209504409 1103 16 0.8700663933328201 1103 17 0.503833722989441 1103 18 0.9125205352451786 1103 19 0.8048066344679015 1103 20 0.5230581058632211 1103 21 0.8678561024106202 1103 22 0.7885935743929835 1103 23 0.9594033277698273 1103 24 0.8133704906389596 1103 25 0.8893104419617259 1104 1 0.5844119881224437 1104 2 0.46530057788499946 1104 3 0.47537363436167723 1104 4 0.49608808584502384 1104 5 0.4491195517591251 1104 6 0.4950318441362924 1104 7 0.5982666017381462 1104 8 0.4470503762933902 1104 9 0.6684573797862541 1104 10 0.6966286541430478 1104 11 0.7537628011938864 1104 12 0.8152593934142018 1104 13 0.9354028207424108 1104 14 1.0133220990885832 1104 15 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-0.9565729265981062 2311 21 -0.9216444904964476 2311 22 -1.0028831523317336 2311 23 -0.991640349271151 2311 24 -1.3234578714653082 2311 25 -1.1727333341201671 2312 1 0.09383288031422055 2312 2 0.058637137279539786 2312 3 0.014496549370512923 2312 4 -0.03129320183906259 2312 5 0.04781761399760853 2312 6 -0.006418844974987792 2312 7 0.139996299878824 2312 8 0.21344014381089238 2312 9 -0.46602352177696815 2312 10 -0.3195119297016049 2312 11 -0.030045140699947126 2312 12 0.15001463266168003 2312 13 0.10955245300178973 2312 14 0.09524110173402534 2312 15 0.07924870832411557 2312 16 0.08528502302971219 2312 17 -0.0473223415541284 2312 18 0.004527613662673957 2312 19 0.09910239479476246 2312 20 -0.05197879697950469 2312 21 0.05037057005704071 2312 22 0.11616764790870349 2312 23 0.042156629459633166 2312 24 0.22216722381521237 2312 25 0.23744024887373172 2313 1 -0.16920152575190173 2313 2 -0.1700271528833568 2313 3 -0.20006303414120907 2313 4 -0.2268132359875139 2313 5 -0.3217436895829593 2313 6 -0.24257911134196064 2313 7 -0.3607403954490209 2313 8 -0.34527587346169497 2313 9 -0.2854509640722469 2313 10 -0.29120262309893974 2313 11 -0.328168951269852 2313 12 -0.201446658542352 2313 13 -0.3104654823216322 2313 14 -0.21299594634648145 2313 15 -0.20755703501489364 2313 16 -0.19080073401870123 2313 17 -0.16450386132198958 2313 18 -0.19955780917656796 2313 19 -0.13281389831406898 2313 20 -0.12720029936053123 2313 21 -0.08575842103909537 2313 22 -0.12397385670606553 2313 23 -0.10694555996563082 2313 24 -0.04223968493617036 2313 25 0.006681893893301094 2314 1 -0.4142707088149855 2314 2 -0.39923859691705843 2314 3 -0.5234284640571722 2314 4 -0.4686113636799476 2314 5 -0.6210580470615383 2314 6 -0.5332041186881824 2314 7 -0.6881260913886962 2314 8 -0.6337010943426264 2314 9 -0.6557919572478994 2314 10 -0.7117794489265492 2314 11 -0.7035765886552099 2314 12 -0.5628173130203109 2314 13 -0.806501704402713 2314 14 -0.6772687065931251 2314 15 -0.6392522686219024 2314 16 -0.6276831935425875 2314 17 -0.6296148856843402 2314 18 -0.6188589856364217 2314 19 -0.7178398351350594 2314 20 -0.6829506017482032 2314 21 -0.6567935610652924 2314 22 -0.49689034792773157 2314 23 -0.5749571828253224 2314 24 -0.6297617460647302 2314 25 -0.5151975324198688 2315 1 -0.25665945974517257 2315 2 -0.3030055443924553 2315 3 -0.36067565890899544 2315 4 -0.3764460971380714 2315 5 -0.39085192761702103 2315 6 -0.3913628337520123 2315 7 -0.43383024900386147 2315 8 -0.2268841782399562 2315 9 -0.37343919024338845 2315 10 -0.48153022192344963 2315 11 -0.43001906989145056 2315 12 -0.4138806981131964 2315 13 -0.5034533904089997 2315 14 -0.608354089603786 2315 15 -0.5793989475328587 2315 16 -0.6181951763553002 2315 17 -0.5565096914847842 2315 18 -0.6434259397303563 2315 19 -0.6511058510082179 2315 20 -0.9666666997216637 2315 21 -0.7668110174493705 2315 22 -0.627302904680576 2315 23 -0.7134622039850385 2315 24 -0.5242862588078054 2315 25 -0.7322767471307827 2316 1 -0.7322851530604737 2316 2 -0.7367689231616872 2316 3 -0.7031544225663602 2316 4 -0.6937122441031889 2316 5 -0.7550051838562268 2316 6 -0.7703903941432557 2316 7 -0.798193715909612 2316 8 -0.7518891169242892 2316 9 -0.9330123866105424 2316 10 -0.949170442568672 2316 11 -1.1171825428755824 2316 12 -1.1221766747316906 2316 13 -0.906424705517324 2316 14 -1.259533548729209 2316 15 -1.258311018690435 2316 16 -1.4569306705529783 2316 17 -1.3052268998871313 2316 18 -1.3256228691350316 2316 19 -1.224476971608361 2316 20 -1.1868763025501394 2316 21 -1.0979156111855986 2316 22 -1.1793667682006657 2316 23 -0.968359099452087 2316 24 -0.8874467128628467 2316 25 -0.8880757756029283 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34342.0 3 3.0 4 631.0 5 5626.0 6 7323.0 7 5929.0 8 4828.0 9 4373.0 10 4012.0 11 3861.0 12 3750.0 13 3571.0 14 3560.0 15 3517.0 16 3699.0 17 3972.0 18 4204.0 19 4770.0 20 5846.0 21 7405.0 22 9842.0 23 12344.0 24 15140.0 25 18347.0 26 21427.0 27 24528.0 28 28516.0 29 35265.0 30 44985.0 31 58284.0 32 75084.0 33 97656.0 34 127566.0 35 168737.0 36 242648.0 37 416062.0 38 905756.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.80362872826284 20.304637054482846 14.298003493315555 24.593730723938766 2 14.138650100169231 21.063502286952684 41.80963999058496 22.988207622293125 3 18.554824607668788 24.995770264775217 30.646076026026215 25.803329101529776 4 12.28000723088232 17.889773720541292 38.901981418948964 30.928237629627425 5 12.664717675610923 38.26200481247685 36.3342416210971 12.73903589081512 6 30.822297757640516 38.32429679876264 18.253013867326548 12.600391576270296 7 27.199907007874963 32.09175609092214 22.4992957335726 18.209041167630303 8 24.082519755655746 38.620440314402735 20.76409080962303 16.53294912031849 9 25.497712633650327 16.053344717422664 21.001948780698672 37.44699386822834 10 14.530598669898225 30.10342891914442 34.635305817095905 20.730666593861443 11 33.44283073323546 22.99755647472149 25.278345534413084 18.281267257629967 12 22.868409938078333 25.069361452695844 32.51758390905304 19.54464470017279 13 28.49112417468455 22.522626498039845 26.503913901205795 22.482335426069813 14 20.09391046364103 23.337507223643165 28.232334702154247 28.336247610561554 15 22.08426459899835 32.30384266791428 23.924871629087175 21.68702110400019 16 21.00347934503429 29.16287645160583 28.948597444619423 20.88504675874045 17 20.61202717454928 29.546055301357775 28.895275892494816 20.94664163159813 18 20.38802701570152 28.42539264146034 31.237494358629426 19.94908598420871 19 22.68585911610323 27.481075812988205 28.423241578069742 21.409823492838818 20 22.477205967215312 28.330787218877735 29.31299585630731 19.879010957599643 21 21.894598721192814 27.601328529843315 28.198703653374334 22.305369095589533 22 21.916978053775757 28.990915480169054 28.66924877006746 20.42285769598773 23 21.30293218896761 28.58560549745616 28.84460180300479 21.26686051057144 24 22.165756808219047 27.586519285731125 29.63536579867122 20.612358107378604 25 21.82013370621039 28.196496831096624 29.27921927487548 20.70415018781751 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 379.0 1 379.0 2 604.5 3 830.0 4 830.0 5 830.0 6 2025.0 7 3220.0 8 3220.0 9 3220.0 10 4894.0 11 6568.0 12 6568.0 13 6568.0 14 10416.5 15 14265.0 16 14265.0 17 14265.0 18 24588.0 19 34911.0 20 34911.0 21 34911.0 22 54908.0 23 74905.0 24 74905.0 25 74905.0 26 106221.5 27 137538.0 28 137538.0 29 137538.0 30 176954.0 31 216370.0 32 216370.0 33 216370.0 34 238084.5 35 259799.0 36 259799.0 37 259799.0 38 273052.5 39 286306.0 40 286306.0 41 286306.0 42 298274.5 43 310243.0 44 310243.0 45 310243.0 46 308170.0 47 306097.0 48 306097.0 49 306097.0 50 288805.0 51 271513.0 52 271513.0 53 271513.0 54 236181.0 55 200849.0 56 200849.0 57 200849.0 58 169152.0 59 137455.0 60 137455.0 61 137455.0 62 110421.5 63 83388.0 64 83388.0 65 83388.0 66 63344.5 67 43301.0 68 43301.0 69 43301.0 70 31181.5 71 19062.0 72 19062.0 73 19062.0 74 13042.0 75 7022.0 76 7022.0 77 7022.0 78 4710.5 79 2399.0 80 2399.0 81 2399.0 82 1552.0 83 705.0 84 705.0 85 705.0 86 456.5 87 208.0 88 208.0 89 208.0 90 136.5 91 65.0 92 65.0 93 65.0 94 36.5 95 8.0 96 8.0 97 8.0 98 5.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4117631728846877 2 0.0 3 0.0 4 0.0 5 0.03201775123696487 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.006990956019440648 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2417409.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.65967260473366 #Duplication Level Percentage of deduplicated Percentage of total 1 85.31959036221404 61.99293502488461 2 10.49000429669741 15.244005556405666 3 2.2988708303640153 5.011056056844647 4 0.7833464725541597 2.2767079292743295 5 0.34196594149429077 1.2423566675472337 6 0.17903609347258506 0.7805222361689114 7 0.1165761208325408 0.5929267941255599 8 0.08379172912603851 0.4870623684225987 9 0.05569975845001445 0.36424135918267175 >10 0.2890988907126565 3.7117971004591266 >50 0.0209547150429536 1.0656427439317109 >100 0.01680104770167393 2.495070724711238 >500 0.00221714549554958 1.1638643401649242 >1k 0.0017623464195324113 2.2983181390755525 >5k 2.842494225052276E-4 1.2734929588012092 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8319 0.34412877589187435 No Hit GTCCTACAGTGGACATTTCTAAATT 5951 0.2461726584123746 No Hit CTGTAGGACGTGGAATATGGCAAGA 5806 0.24017450088090184 No Hit TATCAACGCAGAGTACTTTTTTTTT 5480 0.2266889880860045 No Hit GGTATCAACGCAGAGTACTTTTTTT 5274 0.21816746773094664 No Hit CTTTAGGACGTGAAATATGGCGAGG 4580 0.18945904478720813 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4455 0.18428821932904196 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2757 0.11404772630531283 No Hit TTGTAGAACAGTGTATATCAATGAG 2555 0.10569167236491632 No Hit GTCCTACAGTGTGCATTTCTCATTT 2420 0.10010718087009687 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 4.136660366532928E-5 0.0 11 0.0 0.0 0.0 4.136660366532928E-5 0.0 12 4.136660366532928E-5 0.0 0.0 4.136660366532928E-5 0.0 13 4.136660366532928E-5 8.273320733065856E-5 0.0 4.136660366532928E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATTCGA 25 0.0060432013 18.994457 16 TACCGTA 45 6.779232E-4 14.773466 7 TCCAACG 320 0.0 12.761902 18 TAGGACC 875 0.0 12.59478 4 GGACCGT 55 0.0030760083 12.087381 6 TAGGACT 410 0.0 11.817537 4 CCAACGA 210 0.0 11.758959 19 TGTAGGA 2955 0.0 11.316855 2 AGGACGT 3005 0.0 11.286633 5 GTAGGAC 2950 0.0 11.239424 3 GGACGTG 2930 0.0 11.117916 6 GTACCGT 60 0.005890036 11.080099 6 TTAGGAC 1825 0.0 11.03605 3 GACGTGG 1485 0.0 10.936202 7 AAGGCGA 70 0.0014955573 10.853975 17 TAGGACG 3225 0.0 10.840735 4 GGCGAGG 1355 0.0 10.724251 19 CTGTAGG 3045 0.0 10.554989 1 GCCGGTT 270 0.0 10.552476 11 GACGTGA 1585 0.0 10.545818 7 >>END_MODULE