FastQCFastQC Report
Thu 26 May 2016
SRR1512180_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512180_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439245
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT90850.3724513117788496No Hit
TATCAACGCAGAGTACTTTTTTTTT53110.2177313062033539No Hit
GGTATCAACGCAGAGTACTTTTTTT51700.21195082904751267No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25890.10613939969129792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG604.3811608E-412.565075
GTATAAT3400.010.7973341
GTCCTAC9350.010.2520141
GGTATCA23950.010.13361551
ACACCGT1502.771958E-810.0520556
TAGGACT2750.09.9378284
GTGTAGG3300.09.8884671
ACTATCG3250.09.85854511
TAGGACG9100.09.8380364
TCCAACG1451.8167157E-79.74976118
GTAGGAC8800.09.7450693
TGTAGGA9850.09.628082
GGTCTAC2256.366463E-119.5176511
TCCTACA10950.09.4990242
TTAGACC1502.9781404E-79.4238024
GGACCGT900.00119910169.4238026
AGCGTAC1653.777368E-79.2704381
ACGTGGA5100.09.2390228
GGACGTG9950.09.1870236
CCTATAC1951.9463187E-99.1821673
Produced by FastQC (version 0.11.5)