FastQCFastQC Report
Thu 26 May 2016
SRR1512180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512180_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439245
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT117600.48211639257229183No Hit
TATCAACGCAGAGTACTTTTTTTTT80470.3298971608018055No Hit
GGTATCAACGCAGAGTACTTTTTTT74820.30673425588655506No Hit
ACGCAGAGTACTTTTTTTTTTTTTT40670.1667319190979176No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28940.1186432687163446No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24710.10130183724882086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG500.001503258513.29614118
AAGGCGT1700.012.8492116
GCGTGCG1354.7548383E-911.2559929
GGACCGT1104.9907067E-711.2240156
TATTCCG600.005871150611.0848885
GGCGTGC2150.011.0433058
GGCGAGG4100.010.88797619
AGGCGTG2150.010.6015737
TAGGACG10100.010.44205954
GGTATCA28150.010.4299971
GTCCTAA6000.010.3269951
GCACCGT1201.5302521E-610.2886796
GTAGGAC9500.010.2014313
TTATACG750.002645414810.1347554
GACGCTC951.6495178E-49.9970988
TGGCGAG8850.09.98015418
TTAGCAC2400.09.8972223
AGGACGT10200.09.6876345
TGGACCG1701.6618287E-89.5013335
CGTGCGC1401.0632593E-69.49724310
Produced by FastQC (version 0.11.5)