##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512180_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2439245 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.788548505787652 31.0 31.0 34.0 27.0 34.0 2 30.973747614528264 31.0 31.0 34.0 27.0 34.0 3 31.060360480394547 31.0 31.0 34.0 27.0 34.0 4 34.683958765929624 37.0 35.0 37.0 32.0 37.0 5 34.28173020750273 37.0 35.0 37.0 30.0 37.0 6 34.21137688096112 37.0 35.0 37.0 30.0 37.0 7 34.02934965532368 37.0 35.0 37.0 30.0 37.0 8 33.931536192551384 37.0 35.0 37.0 30.0 37.0 9 35.40884372008552 39.0 35.0 39.0 30.0 39.0 10 35.221652601522194 38.0 35.0 39.0 29.0 39.0 11 35.21082753064985 39.0 35.0 39.0 29.0 39.0 12 35.02177764021244 38.0 35.0 39.0 28.0 39.0 13 34.988417727616536 38.0 35.0 39.0 28.0 39.0 14 35.980513642541034 39.0 36.0 41.0 27.0 41.0 15 35.8756738253025 39.0 36.0 41.0 27.0 41.0 16 35.77910419002601 39.0 36.0 41.0 27.0 41.0 17 35.71318748219223 39.0 36.0 41.0 27.0 41.0 18 35.649316079360624 39.0 36.0 41.0 27.0 41.0 19 35.66837320564355 39.0 36.0 41.0 27.0 41.0 20 35.5431565914863 39.0 35.0 41.0 27.0 41.0 21 35.43014621327501 39.0 35.0 40.0 27.0 41.0 22 35.514299711591086 39.0 36.0 41.0 27.0 41.0 23 35.39920713171494 39.0 35.0 41.0 27.0 41.0 24 35.25796342720801 39.0 35.0 41.0 27.0 41.0 25 34.78748957156825 39.0 34.0 40.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5546002741893474 1101 2 0.5249836098635825 1101 3 0.519259078828604 1101 4 0.5183594380517604 1101 5 0.5597964683077095 1101 6 0.5125251189354287 1101 7 0.5938361176866778 1101 8 0.30211924404344614 1101 9 0.4743489317028846 1101 10 0.6084286138897781 1101 11 0.5517126843335021 1101 12 0.5759890062476742 1101 13 0.5095497082893061 1101 14 0.48499810814155353 1101 15 0.48597421982344713 1101 16 0.5720236933605349 1101 17 0.5541648303946829 1101 18 0.5701816570676073 1101 19 0.5717393819315646 1101 20 0.38529270392547943 1101 21 0.42254764742216366 1101 22 0.3789857069929212 1101 23 0.3610882503514432 1101 24 0.2069515974810301 1101 25 0.34399279953698425 1102 1 0.44310522311527833 1102 2 0.4790546957458197 1102 3 0.4233750676292871 1102 4 0.3868737037329595 1102 5 0.6197408559231476 1102 6 0.628195421057633 1102 7 0.6595762954734141 1102 8 0.7467315181481524 1102 9 0.9192418610540187 1102 10 0.7318961757333398 1102 11 0.8065584055896338 1102 12 0.9978570637042026 1102 13 0.8418676113470269 1102 14 0.8792338220499332 1102 15 1.0107751342101494 1102 16 1.034392358327132 1102 17 1.014329938665945 1102 18 1.1089544386953492 1102 19 1.0294713763415118 1102 20 1.0672945576716302 1102 21 1.0044460563700923 1102 22 1.0450382362038297 1102 23 0.9904744490672712 1102 24 1.054983793113685 1102 25 1.062794299822734 1103 1 0.3680150098840045 1103 2 0.3849759641985244 1103 3 0.3713502817718073 1103 4 0.3951714985206465 1103 5 0.3397080511117778 1103 6 0.39280176332115957 1103 7 0.40637235889604995 1103 8 0.44883558229977893 1103 9 0.7048451050853686 1103 10 0.6196845846999892 1103 11 0.47172880835982056 1103 12 0.5795465114898803 1103 13 0.5454697618846609 1103 14 0.6233572045904836 1103 15 0.8262216441427199 1103 16 0.7085376107419279 1103 17 0.47426706277747854 1103 18 0.7982081292366274 1103 19 0.8470520215028046 1103 20 0.588900170939354 1103 21 0.7804830529073641 1103 22 0.8840814292054731 1103 23 0.876076781853854 1103 24 0.6373056447630319 1103 25 0.7167846406282692 1104 1 0.2154740565296649 1104 2 0.15298834247408166 1104 3 0.28141472829822334 1104 4 0.14727949613583746 1104 5 0.1962989150359178 1104 6 0.25300603325842275 1104 7 0.2805937483290961 1104 8 0.22241747481319152 1104 9 0.4798124559640584 1104 10 0.48203234042777865 1104 11 0.39619151738516933 1104 12 0.508333099798655 1104 13 0.6124295608796331 1104 14 0.6026995377250799 1104 15 0.8191573679595407 1104 16 0.7751666599427338 1104 17 0.8620740354941674 1104 18 0.835901539792232 1104 19 0.8800922225078267 1104 20 0.8775042080741784 1104 21 0.9150009815335451 1104 22 0.7990815711581689 1104 23 0.6977770913107477 1104 24 0.8896166347411736 1104 25 0.8270953750915453 1105 1 0.020554591632791386 1105 2 0.10417162956964177 1105 3 0.14020194005344422 1105 4 0.17643567224073564 1105 5 0.10648729345884078 1105 6 0.13881096708081486 1105 7 0.23719937147708237 1105 8 0.18378876105089148 1105 9 0.18002478424467938 1105 10 0.2788442095717727 1105 11 0.3481266424409455 1105 12 0.22857938405374512 1105 13 0.29817663714196385 1105 14 0.2782886125075308 1105 15 0.28178838361142766 1105 16 0.4834685302107786 1105 17 0.2531697638960253 1105 18 0.4740126732004555 1105 19 0.5935835159449567 1105 20 0.5739449337279723 1105 21 0.48894315017833634 1105 22 0.5506167963440731 1105 23 0.475238744299169 1105 24 0.5025762799550293 1105 25 0.5577115408662152 1106 1 -0.13667632376894545 1106 2 -0.13343685060166877 1106 3 -0.07501453393083679 1106 4 -0.040627593388322225 1106 5 -0.12410649526572826 1106 6 0.039215226757811195 1106 7 -0.08904911379071478 1106 8 -0.16820452035398858 1106 9 -0.12497172562051873 1106 10 -0.21843338252976707 1106 11 -0.2940908707061425 1106 12 -0.2683637510668788 1106 13 -0.17304105342316234 1106 14 -0.25260164929710527 1106 15 -0.42910069997302 1106 16 -0.49337516175588547 1106 17 -0.569771827208136 1106 18 -0.4559438491045569 1106 19 -0.47586811160867626 1106 20 -0.40075689778790746 1106 21 -0.3772589871952903 1106 22 -0.39811350014956304 1106 23 -0.35345591788387054 1106 24 -0.4731825246720902 1106 25 -0.463531358130858 1107 1 -0.07894411274809698 1107 2 -0.12661260343191927 1107 3 -0.025366235343515342 1107 4 -0.06762344996722902 1107 5 -0.15530025729633223 1107 6 -0.14131754541928387 1107 7 -0.18878550085801749 1107 8 -0.026184357879678544 1107 9 -0.04602063103958898 1107 10 0.017449622918668695 1107 11 0.1143509419590174 1107 12 0.01031753176239647 1107 13 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-0.08289622984523959 2314 22 0.0843275435091968 2314 23 -0.05050113817718227 2314 24 -0.06203360501267241 2314 25 -0.12176013277683495 2315 1 -0.39791604692566906 2315 2 -0.434774553448694 2315 3 -0.4773395817851558 2315 4 -0.559149085290997 2315 5 -0.5647393309759465 2315 6 -0.6165258348631966 2315 7 -0.7396678928120153 2315 8 -0.5650355083677283 2315 9 -0.5549728247304344 2315 10 -0.6620670862719749 2315 11 -0.5133005643259452 2315 12 -0.48598155611788485 2315 13 -0.5492278107377047 2315 14 -0.9616659293503176 2315 15 -0.9151380313076558 2315 16 -0.9103888867254781 2315 17 -0.9112467400242252 2315 18 -0.9190362676959367 2315 19 -0.9376574890423655 2315 20 -1.1193238438447892 2315 21 -1.1870712578967968 2315 22 -0.9471659151125493 2315 23 -1.0037380117279895 2315 24 -0.9542986906588737 2315 25 -1.024211164237279 2316 1 -0.7314296460783929 2316 2 -0.7804410689777015 2316 3 -0.7975950897047781 2316 4 -0.7262525716391366 2316 5 -0.8153537194247917 2316 6 -0.8424359974476516 2316 7 -0.8856184772708886 2316 8 -0.8395675081963532 2316 9 -1.1498067217733237 2316 10 -1.1359092264305204 2316 11 -1.0505827619640655 2316 12 -1.2524330339945067 2316 13 -1.0965325618953585 2316 14 -1.3980737581371372 2316 15 -1.4981258594348503 2316 16 -1.4808717946592154 2316 17 -1.4547962700744677 2316 18 -1.3760389397809547 2316 19 -1.4568403100231464 2316 20 -1.4277896897651345 2316 21 -1.3708006057989337 2316 22 -1.3335445078555423 2316 23 -1.3561502396378273 2316 24 -1.0981549756993587 2316 25 -1.2129252807308148 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37626.0 3 1.0 4 646.0 5 6230.0 6 7891.0 7 6246.0 8 5242.0 9 4709.0 10 4518.0 11 4213.0 12 4125.0 13 3908.0 14 3942.0 15 3914.0 16 4011.0 17 4216.0 18 4550.0 19 5153.0 20 6341.0 21 8158.0 22 10519.0 23 13326.0 24 16152.0 25 19204.0 26 22461.0 27 25851.0 28 30130.0 29 37217.0 30 47306.0 31 61429.0 32 79006.0 33 101942.0 34 132334.0 35 174782.0 36 249558.0 37 427292.0 38 865096.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.512607453041326 19.255700264724613 13.988993614254541 24.242698667979518 2 15.542063220381715 20.563002076462183 39.39874838320874 24.49618631994736 3 18.843043646702156 24.667427831152672 29.932581597994464 26.556946924150708 4 13.213309856123514 17.039534774079684 37.02469411641717 32.722461253379635 5 13.764303556291718 37.424004507750155 34.90574200922137 13.905949926736758 6 32.34943599351438 36.659417155718266 17.50877833099996 13.482368519767387 7 28.424287023238747 31.836736367195588 21.48943628048843 18.249540329077234 8 26.01628782676607 35.84863349110073 20.039889392004493 18.095189290128708 9 26.44351018450381 15.693913485525234 19.99278465262817 37.86979167734278 10 15.634714840042719 28.947604689155863 32.997054416428035 22.420626054373383 11 34.64977892749601 22.98125034590621 23.345461403016095 19.023509323581685 12 23.49165417987943 24.7917285881492 30.595819608116447 21.120797623854923 13 28.821336110148838 21.527152869023 25.955859292526988 23.695651728301176 14 21.869348917390422 21.38313289562959 27.369206455276117 29.378311731703867 15 23.75308753323262 29.280986534767926 23.7702649795326 23.19566095246685 16 22.973830017074956 27.939218897650708 26.314125887313494 22.772825197960845 17 22.27726202165014 27.927124991544517 27.182755319781325 22.612857667024016 18 22.089048045604276 27.15331998220761 28.762998386795914 21.9946335853922 19 23.449100028902386 26.87700497490002 27.859932069144342 21.81396292705325 20 23.70959046754221 26.425881778993087 28.13218844355528 21.732339309909417 21 23.5922590801662 26.182937753280218 27.669545289628555 22.555257876925033 22 23.475296659417154 26.43338410040812 27.809588622709075 22.28173061746565 23 23.20304028500622 26.42998140818163 27.771995023050167 22.594983283761984 24 23.04098194318324 26.298055340894415 28.07323577582408 22.587726940098268 25 23.206328954079332 26.51135101981242 27.913357353358403 22.368962672749845 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 818.0 1 818.0 2 958.0 3 1098.0 4 1098.0 5 1098.0 6 2639.5 7 4181.0 8 4181.0 9 4181.0 10 5474.0 11 6767.0 12 6767.0 13 6767.0 14 9261.0 15 11755.0 16 11755.0 17 11755.0 18 20341.0 19 28927.0 20 28927.0 21 28927.0 22 43856.5 23 58786.0 24 58786.0 25 58786.0 26 82027.5 27 105269.0 28 105269.0 29 105269.0 30 131847.5 31 158426.0 32 158426.0 33 158426.0 34 180847.0 35 203268.0 36 203268.0 37 203268.0 38 225555.5 39 247843.0 40 247843.0 41 247843.0 42 270541.5 43 293240.0 44 293240.0 45 293240.0 46 305408.0 47 317576.0 48 317576.0 49 317576.0 50 314699.5 51 311823.0 52 311823.0 53 311823.0 54 286217.0 55 260611.0 56 260611.0 57 260611.0 58 225276.5 59 189942.0 60 189942.0 61 189942.0 62 156723.0 63 123504.0 64 123504.0 65 123504.0 66 95130.5 67 66757.0 68 66757.0 69 66757.0 70 48917.0 71 31077.0 72 31077.0 73 31077.0 74 21477.5 75 11878.0 76 11878.0 77 11878.0 78 7985.0 79 4092.0 80 4092.0 81 4092.0 82 2647.5 83 1203.0 84 1203.0 85 1203.0 86 757.5 87 312.0 88 312.0 89 312.0 90 193.5 91 75.0 92 75.0 93 75.0 94 45.0 95 15.0 96 15.0 97 15.0 98 8.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4070111858382409 2 0.0 3 0.0 4 0.0 5 0.0318951150868404 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.006928373328632425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2439245.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.6997291805535 #Duplication Level Percentage of deduplicated Percentage of total 1 76.51568614144804 48.74028485274471 2 14.396931805793207 18.341613141198486 3 4.592934163432765 8.777059870643374 4 1.8853488972645458 4.8038485666642705 5 0.9195120430460079 2.928633406014408 6 0.5030737653989658 1.9227397568253253 7 0.28800328106311135 1.284201170478172 8 0.1897627123549468 0.9670266708461908 9 0.13485019840718862 0.7730929006633641 >10 0.5299543046161869 5.615639667101367 >50 0.025972574190153093 1.121210355510685 >100 0.015591166785051128 1.9691522124920096 >500 0.0012216210215780694 0.5506571579625811 >1k 9.644376486093543E-4 1.0871844000045456 >5k 1.2859168648124726E-4 0.6360100413513124 >10k+ 6.429584324062363E-5 0.48164582949909424 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 11760 0.48211639257229183 No Hit TATCAACGCAGAGTACTTTTTTTTT 8047 0.3298971608018055 No Hit GGTATCAACGCAGAGTACTTTTTTT 7482 0.30673425588655506 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4067 0.1667319190979176 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2894 0.1186432687163446 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2471 0.10130183724882086 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.199258377079793E-5 2 0.0 0.0 0.0 0.0 8.199258377079793E-5 3 0.0 0.0 0.0 0.0 8.199258377079793E-5 4 0.0 0.0 0.0 0.0 8.199258377079793E-5 5 0.0 4.099629188539897E-5 0.0 0.0 8.199258377079793E-5 6 4.099629188539897E-5 4.099629188539897E-5 0.0 0.0 8.199258377079793E-5 7 4.099629188539897E-5 4.099629188539897E-5 0.0 0.0 8.199258377079793E-5 8 4.099629188539897E-5 4.099629188539897E-5 0.0 0.0 8.199258377079793E-5 9 4.099629188539897E-5 4.099629188539897E-5 0.0 4.099629188539897E-5 1.229888756561969E-4 10 4.099629188539897E-5 4.099629188539897E-5 0.0 4.099629188539897E-5 1.229888756561969E-4 11 8.199258377079793E-5 4.099629188539897E-5 0.0 4.099629188539897E-5 1.229888756561969E-4 12 8.199258377079793E-5 4.099629188539897E-5 0.0 4.099629188539897E-5 2.8697404319779274E-4 13 8.199258377079793E-5 4.099629188539897E-5 0.0 4.099629188539897E-5 3.2797033508319174E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 50 0.0015032585 13.296141 18 AAGGCGT 170 0.0 12.849211 6 GCGTGCG 135 4.7548383E-9 11.255992 9 GGACCGT 110 4.9907067E-7 11.224015 6 TATTCCG 60 0.0058711506 11.084888 5 GGCGTGC 215 0.0 11.043305 8 GGCGAGG 410 0.0 10.887976 19 AGGCGTG 215 0.0 10.601573 7 TAGGACG 1010 0.0 10.4420595 4 GGTATCA 2815 0.0 10.429997 1 GTCCTAA 600 0.0 10.326995 1 GCACCGT 120 1.5302521E-6 10.288679 6 GTAGGAC 950 0.0 10.201431 3 TTATACG 75 0.0026454148 10.134755 4 GACGCTC 95 1.6495178E-4 9.997098 8 TGGCGAG 885 0.0 9.980154 18 TTAGCAC 240 0.0 9.897222 3 AGGACGT 1020 0.0 9.687634 5 TGGACCG 170 1.6618287E-8 9.501333 5 CGTGCGC 140 1.0632593E-6 9.497243 10 >>END_MODULE