FastQCFastQC Report
Thu 26 May 2016
SRR1512179_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512179_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385292
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT54570.2287770218488973No Hit
GTATCAACGCAGAGTACTTTTTTTT54360.22789662649268938No Hit
CTGTAGGACGTGGAATATGGCAAGA48730.20429364622863785No Hit
GTCCTAAAGTGTGTATTTCTCATTT46620.19544776907816735No Hit
CTTTAGGACGTGAAATATGGCGAGG42560.1784267921914801No Hit
GGTATCAACGCAGAGTACTTTTTTT33060.1385993832201676No Hit
TATCAACGCAGAGTACTTTTTTTTT32040.13432317720430037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAC400.005586710814.1292763
CGTTAGG851.6950289E-613.2566071
TAGGACC8700.011.9097354
GGCGAGG13800.011.87753119
GGTATCA14050.011.6654711
CGGTAGG801.875859E-411.5242091
CCAACGA1751.8189894E-1211.30413219
CCTATAC2500.011.3034213
TTCAACG853.2911391E-410.8463142
CGGAGGT700.00160159910.7651634
GGACGTG29950.010.6618256
AGGACCT17950.010.6527145
GTCCTAC26000.010.5589331
GGACCTG16900.010.4785186
AGGACGT31650.010.4165285
GAACAGT5800.010.393956
AATGTCC7700.010.2749768
TAGAACA11350.010.2079354
TGTAGGA27300.010.1686962
GACGTGA16950.010.1141737
Produced by FastQC (version 0.11.5)