FastQCFastQC Report
Thu 26 May 2016
SRR1512179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512179_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385292
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68020.28516424823459774No Hit
GTCCTACAGTGGACATTTCTAAATT62540.262190121796409No Hit
CTGTAGGACGTGGAATATGGCAAGA57720.24198295219201674No Hit
GTCCTAAAGTGTGTATTTCTCATTT53430.2239977327723398No Hit
CTTTAGGACGTGAAATATGGCGAGG49500.2075217625347337No Hit
TATCAACGCAGAGTACTTTTTTTTT44270.1855957258063164No Hit
GGTATCAACGCAGAGTACTTTTTTT41650.1746117456479123No Hit
CTGAAGGACCTGGAATATGGCGAGA26880.11269060559461902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15350.011.5606881
GGCGAGG14750.011.52574719
TAGGACC7800.011.4495144
TCTAGAC2500.011.0207673
TAGACAG3750.010.8940915
TCCAACG2650.010.75118118
AGGACGT31850.010.738585
TGGACCG803.769846E-410.6882435
GGACGTG31050.010.6132576
TAGAACG909.512345E-510.556294
ACTATCG1451.4519173E-810.47931311
TTAGGAC19550.010.4482973
GACGTGG15500.010.3546597
TGTAGGA30050.010.2752572
TGTAGAA10200.010.24581052
GTAGAAC9000.010.2396013
TGGCGAG30950.010.09522618
TAGGACG34450.010.0660124
TAGGACA8500.010.0595234
CTTGCCG856.615665E-410.05551612
Produced by FastQC (version 0.11.5)