FastQCFastQC Report
Thu 26 May 2016
SRR1512178_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512178_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001138
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50840.2540554424532441No Hit
GTCCTACAGTGGACATTTCTAAATT48120.24046317645259846No Hit
CTGTAGGACGTGGAATATGGCAAGA40890.20433373410529407No Hit
GTCCTAAAGTGTGTATTTCTCATTT37440.18709354377359283No Hit
CTTTAGGACGTGAAATATGGCGAGG33670.16825426332416854No Hit
TATCAACGCAGAGTACTTTTTTTTT30600.15291299250726337No Hit
GGTATCAACGCAGAGTACTTTTTTT30560.152713106242548No Hit
GTCCTACAGTGTGCATTTCTCATTT20870.10429065861524793No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG500.001586354313.2013245
TCCAACG2300.012.71030318
TAGGACC7350.012.3161054
GTCCTAT2150.011.7954881
GTCCTAG2600.011.7047551
CCAACGA1701.8189894E-1211.649119
ACCTTTT6000.011.00165415
GGTATCA12000.010.9894651
CGGTAGG750.001534423210.8203961
GAACAGT5200.010.6988756
GTCCTAA13000.010.6903421
CTGTAGG22500.010.6851411
GTATCAA24800.010.6758691
TGTAGGA23300.010.6665652
GGCGAGG10100.010.45727919
AGGACGT23800.010.2615335
GTCTAGG1903.2014214E-1010.1441211
GTAGGAC24600.010.0811513
GACAGCG750.002816823510.0581527
TATGCCG750.002816823510.0581525
Produced by FastQC (version 0.11.5)